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- Antigen structure
- References [41]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 12270-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12270-1-AP, RRID:AB_2147525
- Product name
- MTHFD2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated MTHFD2 antibody (Cat. #12270-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references DELE1 promotes translation-associated homeostasis, growth, and survival in mitochondrial myopathy.
SNHG3/hsa-miR-455-5p Axis-mediated High Expression of MTHFD2 Correlates with Tumor Immune Infiltration and Endometrial Carcinoma Progression.
Overexpression of MTHFD2 represents an inflamed tumor microenvironment and precisely predicts the molecular subtype and immunotherapy response of bladder cancer.
Comparative multi-omic analyses of cardiac mitochondrial stress in three mouse models of frataxin deficiency.
Mitochondrial One-Carbon Metabolism is Required for TGF-β-Induced Glycine Synthesis and Collagen Protein Production.
MTHFD2 reprograms macrophage polarization by inhibiting PTEN.
Mitochondrial folate pathway regulates myofibroblast differentiation and silica-induced pulmonary fibrosis.
A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.
A mouse model of human mitofusin-2-related lipodystrophy exhibits adipose-specific mitochondrial stress and reduced leptin secretion.
MTHFD2 suppresses glioblastoma progression via the inhibition of ERK1/2 phosphorylation.
Cyclin D1 extensively reprograms metabolism to support biosynthetic pathways in hepatocytes.
PRMT1 orchestrates with SAMTOR to govern mTORC1 methionine sensing via Arg-methylation of NPRL2.
SLC25A51 promotes tumor growth through sustaining mitochondria acetylation homeostasis and proline biogenesis.
ATF4/MYC Regulates MTHFD2 to Promote NSCLC Progression by Mediating Redox Homeostasis.
Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.
An LKB1-mitochondria axis controls T(H)17 effector function.
Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.
Adaptation of the heart to Frataxin depletion: Evidence that integrated stress response can predominate over mTORC1 activation.
Hepatic mTORC1 signaling activates ATF4 as part of its metabolic response to feeding and insulin.
The mTORC1-mediated activation of ATF4 promotes protein and glutathione synthesis downstream of growth signals.
ATF3 coordinates serine and nucleotide metabolism to drive cell cycle progression in acute myeloid leukemia.
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.
The Prognostic Significance of Immune-Related Metabolic Enzyme MTHFD2 in Head and Neck Squamous Cell Carcinoma.
Loss of CHCHD2 and CHCHD10 activates OMA1 peptidase to disrupt mitochondrial cristae phenocopying patient mutations.
mTOR Overcomes Multiple Metabolic Restrictions to Enable HIV-1 Reverse Transcription and Intracellular Transport.
Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.
The effect of MTHFD2 on the proliferation and migration of colorectal cancer cell lines.
De novo synthesis of serine and glycine fuels purine nucleotide biosynthesis in human lung cancer tissues.
Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation.
Two separate functions of NME3 critical for cell survival underlie a neurodegenerative disorder.
mTOR-S6K1 pathway mediates cytoophidium assembly.
Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.
Histidine-Dependent Protein Methylation Is Required for Compartmentalization of CTP Synthase.
Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.
Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells.
mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.
mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.
Oxidative stress inhibits distant metastasis by human melanoma cells.
Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus.
Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.
Lin HP, Petersen JD, Gilsrud AJ, Madruga A, D'Silva TM, Huang X, Shammas MK, Randolph NP, Li Y, Jones DR, Pacold ME, Narendra DP
bioRxiv : the preprint server for biology 2024 Feb 29;
bioRxiv : the preprint server for biology 2024 Feb 29;
SNHG3/hsa-miR-455-5p Axis-mediated High Expression of MTHFD2 Correlates with Tumor Immune Infiltration and Endometrial Carcinoma Progression.
Wu S, Cai W, Li Y, Tan W, Yuan Y, Zhou Z, Shi J, Liu X, Gao H
International journal of medical sciences 2023;20(8):1097-1113
International journal of medical sciences 2023;20(8):1097-1113
Overexpression of MTHFD2 represents an inflamed tumor microenvironment and precisely predicts the molecular subtype and immunotherapy response of bladder cancer.
Shi X, Peng X, Chen Y, Shi Z, Yue C, Zuo L, Zhang L, Gao S
Frontiers in immunology 2023;14:1326509
Frontiers in immunology 2023;14:1326509
Comparative multi-omic analyses of cardiac mitochondrial stress in three mouse models of frataxin deficiency.
Sayles NM, Napierala JS, Anrather J, Diedhiou N, Li J, Napierala M, Puccio H, Manfredi G
Disease models & mechanisms 2023 Oct 1;16(10)
Disease models & mechanisms 2023 Oct 1;16(10)
Mitochondrial One-Carbon Metabolism is Required for TGF-β-Induced Glycine Synthesis and Collagen Protein Production.
Meliton AY, Cetin-Atalay R, Tian Y, Szafran JCH, Shin KWD, Cho T, Sun KA, Woods PS, Shamaa OR, Chen B, Muir A, Mutlu GM, Hamanaka RB
bioRxiv : the preprint server for biology 2023 Nov 7;
bioRxiv : the preprint server for biology 2023 Nov 7;
MTHFD2 reprograms macrophage polarization by inhibiting PTEN.
Shang M, Ni L, Shan X, Cui Y, Hu P, Ji Z, Shen L, Zhang Y, Zhou J, Bing Chen, Wang T, Yu Q
Cell reports 2023 May 30;42(5):112481
Cell reports 2023 May 30;42(5):112481
Mitochondrial folate pathway regulates myofibroblast differentiation and silica-induced pulmonary fibrosis.
Qu Y, Zhai R, Wang D, Wang Z, Hou G, Wu C, Tang M, Xiao X, Jiao J, Ba Y, Zhou F, Qiu J, Yao W
Journal of translational medicine 2023 Jun 6;21(1):365
Journal of translational medicine 2023 Jun 6;21(1):365
A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.
Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
EMBO molecular medicine 2023 Jul 10;15(7):e16951
EMBO molecular medicine 2023 Jul 10;15(7):e16951
A mouse model of human mitofusin-2-related lipodystrophy exhibits adipose-specific mitochondrial stress and reduced leptin secretion.
Mann JP, Duan X, Patel S, Tábara LC, Scurria F, Alvarez-Guaita A, Haider A, Luijten I, Page M, Protasoni M, Lim K, Virtue S, O'Rahilly S, Armstrong M, Prudent J, Semple RK, Savage DB
eLife 2023 Feb 1;12
eLife 2023 Feb 1;12
MTHFD2 suppresses glioblastoma progression via the inhibition of ERK1/2 phosphorylation.
Huang M, Xue J, Chen Z, Zhou X, Chen M, Sun J, Xu Z, Wang S, Xu H, Du Z, Liu M
Biochemistry and cell biology = Biochimie et biologie cellulaire 2023 Feb 1;101(1):112-124
Biochemistry and cell biology = Biochimie et biologie cellulaire 2023 Feb 1;101(1):112-124
Cyclin D1 extensively reprograms metabolism to support biosynthetic pathways in hepatocytes.
Wu H, Kren BT, Lane AN, Cassel TA, Higashi RM, Fan TWM, Scaria GS, Shekels LL, Klein MA, Albrecht JH
The Journal of biological chemistry 2023 Dec;299(12):105407
The Journal of biological chemistry 2023 Dec;299(12):105407
PRMT1 orchestrates with SAMTOR to govern mTORC1 methionine sensing via Arg-methylation of NPRL2.
Jiang C, Liu J, He S, Xu W, Huang R, Pan W, Li X, Dai X, Guo J, Zhang T, Inuzuka H, Wang P, Asara JM, Xiao J, Wei W
Cell metabolism 2023 Dec 5;35(12):2183-2199.e7
Cell metabolism 2023 Dec 5;35(12):2183-2199.e7
SLC25A51 promotes tumor growth through sustaining mitochondria acetylation homeostasis and proline biogenesis.
Li Y, Bie J, Zhao L, Song C, Zhang T, Li M, Yang C, Luo J
Cell death and differentiation 2023 Aug;30(8):1916-1930
Cell death and differentiation 2023 Aug;30(8):1916-1930
ATF4/MYC Regulates MTHFD2 to Promote NSCLC Progression by Mediating Redox Homeostasis.
Gao Y, Feng L, Zhang L, Geng J, Zhang E
Disease markers 2022;2022:7527996
Disease markers 2022;2022:7527996
Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.
Höllerhage M, Stepath M, Kohl M, Pfeiffer K, Chua OWH, Duan L, Hopfner F, Eisenacher M, Marcus K, Höglinger GU
Frontiers in neurology 2022;13:787059
Frontiers in neurology 2022;13:787059
An LKB1-mitochondria axis controls T(H)17 effector function.
Baixauli F, Piletic K, Puleston DJ, Villa M, Field CS, Flachsmann LJ, Quintana A, Rana N, Edwards-Hicks J, Matsushita M, Stanczak MA, Grzes KM, Kabat AM, Fabri M, Caputa G, Kelly B, Corrado M, Musa Y, Duda KJ, Mittler G, O'Sullivan D, Sesaki H, Jenuwein T, Buescher JM, Pearce EJ, Sanin DE, Pearce EL
Nature 2022 Oct;610(7932):555-561
Nature 2022 Oct;610(7932):555-561
Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.
Fiore A, Zeitler L, Russier M, Groß A, Hiller MK, Parker JL, Stier L, Köcher T, Newstead S, Murray PJ
Molecular cell 2022 Mar 3;82(5):920-932.e7
Molecular cell 2022 Mar 3;82(5):920-932.e7
Adaptation of the heart to Frataxin depletion: Evidence that integrated stress response can predominate over mTORC1 activation.
Vásquez-Trincado C, Patel M, Sivaramakrishnan A, Bekeová C, Anderson-Pullinger L, Wang N, Tang HY, Seifert EL
Human molecular genetics 2021 Sep 22;33(8):637-54
Human molecular genetics 2021 Sep 22;33(8):637-54
Hepatic mTORC1 signaling activates ATF4 as part of its metabolic response to feeding and insulin.
Byles V, Cormerais Y, Kalafut K, Barrera V, Hughes Hallett JE, Sui SH, Asara JM, Adams CM, Hoxhaj G, Ben-Sahra I, Manning BD
Molecular metabolism 2021 Nov;53:101309
Molecular metabolism 2021 Nov;53:101309
The mTORC1-mediated activation of ATF4 promotes protein and glutathione synthesis downstream of growth signals.
Torrence ME, MacArthur MR, Hosios AM, Valvezan AJ, Asara JM, Mitchell JR, Manning BD
eLife 2021 Mar 1;10
eLife 2021 Mar 1;10
ATF3 coordinates serine and nucleotide metabolism to drive cell cycle progression in acute myeloid leukemia.
Di Marcantonio D, Martinez E, Kanefsky JS, Huhn JM, Gabbasov R, Gupta A, Krais JJ, Peri S, Tan Y, Skorski T, Dorrance A, Garzon R, Goldman AR, Tang HY, Johnson N, Sykes SM
Molecular cell 2021 Jul 1;81(13):2752-2764.e6
Molecular cell 2021 Jul 1;81(13):2752-2764.e6
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
Cui L, Zhao X, Jin Z, Wang H, Yang SF, Hu S
Journal of pineal research 2021 Dec;71(4):e12767
Journal of pineal research 2021 Dec;71(4):e12767
Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.
Shao C, Lu W, Du Y, Yan W, Bao Q, Tian Y, Wang G, Ye H, Hao H
Redox biology 2020 Sep;36:101685
Redox biology 2020 Sep;36:101685
The Prognostic Significance of Immune-Related Metabolic Enzyme MTHFD2 in Head and Neck Squamous Cell Carcinoma.
Cui L, Chen H, Zhao X
Diagnostics (Basel, Switzerland) 2020 Sep 11;10(9)
Diagnostics (Basel, Switzerland) 2020 Sep 11;10(9)
Loss of CHCHD2 and CHCHD10 activates OMA1 peptidase to disrupt mitochondrial cristae phenocopying patient mutations.
Liu YT, Huang X, Nguyen D, Shammas MK, Wu BP, Dombi E, Springer DA, Poulton J, Sekine S, Narendra DP
Human molecular genetics 2020 Jun 3;29(9):1547-1567
Human molecular genetics 2020 Jun 3;29(9):1547-1567
mTOR Overcomes Multiple Metabolic Restrictions to Enable HIV-1 Reverse Transcription and Intracellular Transport.
Taylor HE, Calantone N, Lichon D, Hudson H, Clerc I, Campbell EM, D'Aquila RT
Cell reports 2020 Jun 23;31(12):107810
Cell reports 2020 Jun 23;31(12):107810
Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.
Balsa E, Perry EA, Bennett CF, Jedrychowski M, Gygi SP, Doench JG, Puigserver P
Nature communications 2020 Jun 1;11(1):2714
Nature communications 2020 Jun 1;11(1):2714
The effect of MTHFD2 on the proliferation and migration of colorectal cancer cell lines.
Wei Y, Liu P, Li Q, Du J, Chen Y, Wang Y, Shi H, Wang Y, Zhang H, Xue W, Gao Y, Li D, Feng Y, Yan J, Han J, Zhang J
OncoTargets and therapy 2019;12:6361-6370
OncoTargets and therapy 2019;12:6361-6370
De novo synthesis of serine and glycine fuels purine nucleotide biosynthesis in human lung cancer tissues.
Fan TWM, Bruntz RC, Yang Y, Song H, Chernyavskaya Y, Deng P, Zhang Y, Shah PP, Beverly LJ, Qi Z, Mahan AL, Higashi RM, Dang CV, Lane AN
The Journal of biological chemistry 2019 Sep 6;294(36):13464-13477
The Journal of biological chemistry 2019 Sep 6;294(36):13464-13477
Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation.
Wang LW, Shen H, Nobre L, Ersing I, Paulo JA, Trudeau S, Wang Z, Smith NA, Ma Y, Reinstadler B, Nomburg J, Sommermann T, Cahir-McFarland E, Gygi SP, Mootha VK, Weekes MP, Gewurz BE
Cell metabolism 2019 Sep 3;30(3):539-555.e11
Cell metabolism 2019 Sep 3;30(3):539-555.e11
Two separate functions of NME3 critical for cell survival underlie a neurodegenerative disorder.
Chen CW, Wang HL, Huang CW, Huang CY, Lim WK, Tu IC, Koorapati A, Hsieh ST, Kan HW, Tzeng SR, Liao JC, Chong WM, Naroditzky I, Kidron D, Eran A, Nijim Y, Sela E, Feldman HB, Kalfon L, Raveh-Barak H, Falik-Zaccai TC, Elpeleg O, Mandel H, Chang ZF
Proceedings of the National Academy of Sciences of the United States of America 2019 Jan 8;116(2):566-574
Proceedings of the National Academy of Sciences of the United States of America 2019 Jan 8;116(2):566-574
mTOR-S6K1 pathway mediates cytoophidium assembly.
Sun Z, Liu JL
Journal of genetics and genomics = Yi chuan xue bao 2019 Feb;46(2):65-74
Journal of genetics and genomics = Yi chuan xue bao 2019 Feb;46(2):65-74
Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.
Koufaris C, Nilsson R
Cancer & metabolism 2018;6:12
Cancer & metabolism 2018;6:12
Histidine-Dependent Protein Methylation Is Required for Compartmentalization of CTP Synthase.
Lin WC, Chakraborty A, Huang SC, Wang PY, Hsieh YJ, Chien KY, Lee YH, Chang CC, Tang HY, Lin YT, Tung CS, Luo JD, Chen TW, Lin TY, Cheng ML, Chen YT, Yeh CT, Liu JL, Sung LY, Shiao MS, Yu JS, Chang YS, Pai LM
Cell reports 2018 Sep 4;24(10):2733-2745.e7
Cell reports 2018 Sep 4;24(10):2733-2745.e7
Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.
Dogan SA, Cerutti R, Benincá C, Brea-Calvo G, Jacobs HT, Zeviani M, Szibor M, Viscomi C
Cell metabolism 2018 Nov 6;28(5):764-775.e5
Cell metabolism 2018 Nov 6;28(5):764-775.e5
Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells.
Hitzel J, Lee E, Zhang Y, Bibli SI, Li X, Zukunft S, Pflüger B, Hu J, Schürmann C, Vasconez AE, Oo JA, Kratzer A, Kumar S, Rezende F, Josipovic I, Thomas D, Giral H, Schreiber Y, Geisslinger G, Fork C, Yang X, Sigala F, Romanoski CE, Kroll J, Jo H, Landmesser U, Lusis AJ, Namgaladze D, Fleming I, Leisegang MS, Zhu J, Brandes RP
Nature communications 2018 Jun 12;9(1):2292
Nature communications 2018 Jun 12;9(1):2292
mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.
Khan NA, Nikkanen J, Yatsuga S, Jackson C, Wang L, Pradhan S, Kivelä R, Pessia A, Velagapudi V, Suomalainen A
Cell metabolism 2017 Aug 1;26(2):419-428.e5
Cell metabolism 2017 Aug 1;26(2):419-428.e5
mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.
Ben-Sahra I, Hoxhaj G, Ricoult SJH, Asara JM, Manning BD
Science (New York, N.Y.) 2016 Feb 12;351(6274):728-733
Science (New York, N.Y.) 2016 Feb 12;351(6274):728-733
Oxidative stress inhibits distant metastasis by human melanoma cells.
Piskounova E, Agathocleous M, Murphy MM, Hu Z, Huddlestun SE, Zhao Z, Leitch AM, Johnson TM, DeBerardinis RJ, Morrison SJ
Nature 2015 Nov 12;527(7577):186-91
Nature 2015 Nov 12;527(7577):186-91
Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus.
Sugiyama K, Ebinuma H, Nakamoto N, Sakasegawa N, Murakami Y, Chu PS, Usui S, Ishibashi Y, Wakayama Y, Taniki N, Murata H, Saito Y, Fukasawa M, Saito K, Yamagishi Y, Wakita T, Takaku H, Hibi T, Saito H, Kanai T
PloS one 2014;9(4):e94460
PloS one 2014;9(4):e94460
Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.
Mitsuishi Y, Taguchi K, Kawatani Y, Shibata T, Nukiwa T, Aburatani H, Yamamoto M, Motohashi H
Cancer cell 2012 Jul 10;22(1):66-79
Cancer cell 2012 Jul 10;22(1):66-79
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12270-1-AP(MTHFD2 antibody) at dilution of 1:300
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The MTHFD2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MTHFD2. This antibody recognizes human, mouse, rat antigen. The MTHFD2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
