12270-1-AP

antibody from Proteintech Group

Targeting: MTHFD2

Provider product page for 12270-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [60]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 12270-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12270-1-AP, RRID:AB_2147525
Product name: MTHFD2 antibody
Antibody type: Polyclonal
Description: KD/KO validated MTHFD2 antibody (Cat. #12270-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MTHFD2 protein structure - 12270-1-AP shown in red.

Submitted references Txnrd2 loss in skeletal muscle causes muscle atrophy and drives leanness and obesity resistance.

Kiermayer C, Erdelen R, Schriever SC, Böhm R, Prehn C, Artati A, Kleinert M, Miller M, Dyar KA, Schmid RM, Pfluger PT, Adamski J, Brielmeier M
Redox biology 2026 Jun;93:104165

Integration of Bulk and Single-Cell Transcriptomics Reveals Prognostic and Immunological Roles of MTHFD2 in Clear Cell Renal Cell Carcinoma.

Zhou Y, Zheng X, Ye P, Yang H
International journal of molecular sciences 2026 Feb 20;27(4)

Mitochondrial protein import stress causes lysosomal damage and progressive tissue atrophy.

Brennan NA, Wang X, Rana A, Bhagwagar SZ, Horton J, Kane PM, Chen XJ
EMBO reports 2026 Apr 27;

Clinical, neuropathological, and biochemical characterization of ALS in a large CHCHD10 R15L family.

Kwan JY, Lantz CI, Korobeynikov VA, Snyder A, Huang X, Haselhuhn T, Dore KN, Madruga A, Danielian LE, Schindler AB, Chia R, Rasheed M, Crook J, Szabo M, Portley M, Sherer CM, King MC, Huang TH, Kosa P, Bielekova B, Ward ME, Grunseich C, Shneider NA, Traynor BJ, Narendra DP
medRxiv : the preprint server for health sciences 2025 Sep 26;

SIRT5-mediated desuccinylation of MTHFD2 enhances chemoresistance in breast cancer cells by reducing therapy-induced senescence.

Xianhong Z, Yue G, Yu Z, Yichen Z, Yufei C, Xinke P, Jinhui Z, Yiqi W, Yitian D, Shuailin H, Ting N
Communications biology 2025 Oct 20;8(1):1485

Mitochondrial one-carbon metabolism is required for TGF-β-induced glycine synthesis and fibrotic responses.

Meliton AY, Shin KWD, Cetin-Atalay R, Atalay MV, Tian Y, Houpy Szafran JC, Cho T, Sun KA, Woods PS, Shamaa OR, Chen B, Dulin NO, Husain AN, Muir A, Shah H, Mutlu GM, Hamanaka RB
Nature communications 2025 Oct 20;16(1):9250

Autoimmune disease risk gene ANKRD55 promotes TH17 effector function through metabolic modulation.

Xu J, Kong L, Creasey EA, Rath S, Deng L, Avila-Pacheco J, Li C, Oliver BA, Dao TT, Shih AR, Daly MJ, Shalek AK, Clish CB, Graham DB, Deguine J, Xavier RJ
The Journal of experimental medicine 2025 Nov 3;222(11)

Programmable initiation of mRNA translation by trans-RNA.

Jia L, Nguyen TT, Uematsu S, Gu Y, Shi S, Hashem Y, Qian SB
Nature biotechnology 2025 Nov 21;

MTHFD2 promotes esophageal squamous cell carcinoma progression via m6A modification‑mediated upregulation and modulation of the PEBP1‑RAF1 interaction.

Zhou H, Gong H, Zeng X, Zeng C, Liu D, Liu J, Zhang Y
International journal of molecular medicine 2025 May;55(5)

MTHFD2 promotes osteoclastogenesis and bone loss in rheumatoid arthritis by enhancing CKMT1-mediated oxidative phosphorylation.

Li Y, Cai M, Qin Y, Dai X, Liang L, Li Z, Wen X, Jin H, Yang C, Chen Z
BMC medicine 2025 Feb 27;23(1):124

MTHFD2: A metabolic checkpoint altering trophoblast invasion and migration by remodeling folate-nucleotide metabolism in recurrent spontaneous abortion.

Xu Q, Zhou Y, Wu M, Wu S, Yu J, Xu Y, Wei Z, Jin L
Cellular signalling 2025 Aug;132:111808

Hit to lead optimization of isopentenyl chalcones as novel MTHFD2 inhibitors for cancer treatment: design, synthesis, in-vitro, in-vivo and in-silico studies.

Hu Y, He X, Li S, Zhang T, Liao J, Xu N, Yuan Y, Wang Q, Chen Z, Huang J, Ma L
European journal of medicinal chemistry 2025 Aug 5;292:117703

DKC1-mediated pseudouridylation of rRNA targets hnRNP A1 to sustain IRES-dependent translation and ATF4-driven metabolic adaptation.

Gupta A, Bansal M, Ding J, Karki S, Pandit M, Sudarshan S, Ding HF
Science advances 2025 Aug 29;11(35):eadv9401

Atf3 controls transitioning in female mitochondrial cardiomyopathy as identified by spatial and single-cell transcriptomics.

Qaqorh T, Takahashi Y, Sameshima K, Otani K, Yazawa I, Nishida Y, Tonai K, Fujihara Y, Honda M, Oki S, Ohkawa Y, Thorburn DR, Frazier AE, Takeda A, Ikeda Y, Sakaguchi H, Watanabe T, Fukushima N, Tsukamoto Y, Makita N, Yamaguchi O, Murayama K, Ohtake A, Okazaki Y, Kimura T, Kato H, Inoue H, Matsuoka K, Takashima S, Shintani Y
Science advances 2025 Apr 4;11(14):eadq1575

Asparagine Dependency Is a Targetable Metabolic Vulnerability in TP53-Altered Castration-Resistant Prostate Cancer.

Yoo YA, Quan S, Yang W, Guo Q, Rodríguez Y, Chalmers ZR, Dufficy MF, Lackie B, Sagar V, Unno K, Truica MI, Chandel NS, Abdulkadir SA
Cancer research 2024 Sep 16;84(18):3004-3022

DELE1 maintains muscle proteostasis to promote growth and survival in mitochondrial myopathy.

Lin HP, Petersen JD, Gilsrud AJ, Madruga A, D'Silva TM, Huang X, Shammas MK, Randolph NP, Johnson KR, Li Y, Jones DR, Pacold ME, Narendra DP
The EMBO journal 2024 Nov;43(22):5548-5585

DELE1 promotes translation-associated homeostasis, growth, and survival in mitochondrial myopathy.

Lin HP, Petersen JD, Gilsrud AJ, Madruga A, D'Silva TM, Huang X, Shammas MK, Randolph NP, Li Y, Jones DR, Pacold ME, Narendra DP
bioRxiv : the preprint server for biology 2024 Feb 29;

DKC1-mediated pseudouridylation of rRNA targets hnRNP A1 to sustain IRES-dependent translation and ATF4-driven metabolic adaptation.

Gupta A, Bansal M, Ding J, Pandit M, Sudarshan S, Ding HF
bioRxiv : the preprint server for biology 2024 Dec 17;

Opa1 processing is dispensable in mouse development but is protective in mitochondrial cardiomyopathy.

Ahola S, Pazurek LA, Mayer F, Lampe P, Hermans S, Becker L, Amarie OV, Fuchs H, Gailus-Durner V, de Angelis MH, Riedel D, Nolte H, Langer T
Science advances 2024 Aug 2;10(31):eadp0443

Induction of MTHFD2 in Macrophages Inhibits Reactive Oxygen Species-mediated NF-κB Activation and Protects against Inflammatory Responses.

Cui Y, Li Z, Ni L, Yu S, Shan X, Hu P, Ji Z, Jing W, Zhou Y, Wang B, Dong H, Zhou J, Xie K, Yu Q
Journal of immunology (Baltimore, Md. : 1950) 2024 Apr 15;212(8):1345-1356

SNHG3/hsa-miR-455-5p Axis-mediated High Expression of MTHFD2 Correlates with Tumor Immune Infiltration and Endometrial Carcinoma Progression.

Wu S, Cai W, Li Y, Tan W, Yuan Y, Zhou Z, Shi J, Liu X, Gao H
International journal of medical sciences 2023;20(8):1097-1113

Overexpression of MTHFD2 represents an inflamed tumor microenvironment and precisely predicts the molecular subtype and immunotherapy response of bladder cancer.

Shi X, Peng X, Chen Y, Shi Z, Yue C, Zuo L, Zhang L, Gao S
Frontiers in immunology 2023;14:1326509

Comparative multi-omic analyses of cardiac mitochondrial stress in three mouse models of frataxin deficiency.

Sayles NM, Napierala JS, Anrather J, Diedhiou N, Li J, Napierala M, Puccio H, Manfredi G
Disease models & mechanisms 2023 Oct 1;16(10)

Mitochondrial One-Carbon Metabolism is Required for TGF-β-Induced Glycine Synthesis and Collagen Protein Production.

Meliton AY, Cetin-Atalay R, Tian Y, Szafran JCH, Shin KWD, Cho T, Sun KA, Woods PS, Shamaa OR, Chen B, Muir A, Mutlu GM, Hamanaka RB
bioRxiv : the preprint server for biology 2023 Nov 7;

MTHFD2 reprograms macrophage polarization by inhibiting PTEN.

Shang M, Ni L, Shan X, Cui Y, Hu P, Ji Z, Shen L, Zhang Y, Zhou J, Bing Chen, Wang T, Yu Q
Cell reports 2023 May 30;42(5):112481

Mitochondrial folate pathway regulates myofibroblast differentiation and silica-induced pulmonary fibrosis.

Qu Y, Zhai R, Wang D, Wang Z, Hou G, Wu C, Tang M, Xiao X, Jiao J, Ba Y, Zhou F, Qiu J, Yao W
Journal of translational medicine 2023 Jun 6;21(1):365

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
EMBO molecular medicine 2023 Jul 10;15(7):e16951

A mouse model of human mitofusin-2-related lipodystrophy exhibits adipose-specific mitochondrial stress and reduced leptin secretion.

Mann JP, Duan X, Patel S, Tábara LC, Scurria F, Alvarez-Guaita A, Haider A, Luijten I, Page M, Protasoni M, Lim K, Virtue S, O'Rahilly S, Armstrong M, Prudent J, Semple RK, Savage DB
eLife 2023 Feb 1;12

MTHFD2 suppresses glioblastoma progression via the inhibition of ERK1/2 phosphorylation.

Huang M, Xue J, Chen Z, Zhou X, Chen M, Sun J, Xu Z, Wang S, Xu H, Du Z, Liu M
Biochemistry and cell biology = Biochimie et biologie cellulaire 2023 Feb 1;101(1):112-124

Cyclin D1 extensively reprograms metabolism to support biosynthetic pathways in hepatocytes.

Wu H, Kren BT, Lane AN, Cassel TA, Higashi RM, Fan TWM, Scaria GS, Shekels LL, Klein MA, Albrecht JH
The Journal of biological chemistry 2023 Dec;299(12):105407

PRMT1 orchestrates with SAMTOR to govern mTORC1 methionine sensing via Arg-methylation of NPRL2.

Jiang C, Liu J, He S, Xu W, Huang R, Pan W, Li X, Dai X, Guo J, Zhang T, Inuzuka H, Wang P, Asara JM, Xiao J, Wei W
Cell metabolism 2023 Dec 5;35(12):2183-2199.e7

SLC25A51 promotes tumor growth through sustaining mitochondria acetylation homeostasis and proline biogenesis.

Li Y, Bie J, Zhao L, Song C, Zhang T, Li M, Yang C, Luo J
Cell death and differentiation 2023 Aug;30(8):1916-1930

ATF4/MYC Regulates MTHFD2 to Promote NSCLC Progression by Mediating Redox Homeostasis.

Gao Y, Feng L, Zhang L, Geng J, Zhang E
Disease markers 2022;2022:7527996

Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.

Höllerhage M, Stepath M, Kohl M, Pfeiffer K, Chua OWH, Duan L, Hopfner F, Eisenacher M, Marcus K, Höglinger GU
Frontiers in neurology 2022;13:787059

An LKB1-mitochondria axis controls T(H)17 effector function.

Baixauli F, Piletic K, Puleston DJ, Villa M, Field CS, Flachsmann LJ, Quintana A, Rana N, Edwards-Hicks J, Matsushita M, Stanczak MA, Grzes KM, Kabat AM, Fabri M, Caputa G, Kelly B, Corrado M, Musa Y, Duda KJ, Mittler G, O'Sullivan D, Sesaki H, Jenuwein T, Buescher JM, Pearce EJ, Sanin DE, Pearce EL
Nature 2022 Oct;610(7932):555-561

Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.

Fiore A, Zeitler L, Russier M, Groß A, Hiller MK, Parker JL, Stier L, Köcher T, Newstead S, Murray PJ
Molecular cell 2022 Mar 3;82(5):920-932.e7

Adaptation of the heart to Frataxin depletion: Evidence that integrated stress response can predominate over mTORC1 activation.

Vásquez-Trincado C, Patel M, Sivaramakrishnan A, Bekeová C, Anderson-Pullinger L, Wang N, Tang HY, Seifert EL
Human molecular genetics 2021 Sep 22;33(8):637-54

Hepatic mTORC1 signaling activates ATF4 as part of its metabolic response to feeding and insulin.

Byles V, Cormerais Y, Kalafut K, Barrera V, Hughes Hallett JE, Sui SH, Asara JM, Adams CM, Hoxhaj G, Ben-Sahra I, Manning BD
Molecular metabolism 2021 Nov;53:101309

The mTORC1-mediated activation of ATF4 promotes protein and glutathione synthesis downstream of growth signals.

Torrence ME, MacArthur MR, Hosios AM, Valvezan AJ, Asara JM, Mitchell JR, Manning BD
eLife 2021 Mar 1;10

ATF3 coordinates serine and nucleotide metabolism to drive cell cycle progression in acute myeloid leukemia.

Di Marcantonio D, Martinez E, Kanefsky JS, Huhn JM, Gabbasov R, Gupta A, Krais JJ, Peri S, Tan Y, Skorski T, Dorrance A, Garzon R, Goldman AR, Tang HY, Johnson N, Sykes SM
Molecular cell 2021 Jul 1;81(13):2752-2764.e6

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Cui L, Zhao X, Jin Z, Wang H, Yang SF, Hu S
Journal of pineal research 2021 Dec;71(4):e12767

Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.

Shao C, Lu W, Du Y, Yan W, Bao Q, Tian Y, Wang G, Ye H, Hao H
Redox biology 2020 Sep;36:101685

The Prognostic Significance of Immune-Related Metabolic Enzyme MTHFD2 in Head and Neck Squamous Cell Carcinoma.

Cui L, Chen H, Zhao X
Diagnostics (Basel, Switzerland) 2020 Sep 11;10(9)

Loss of CHCHD2 and CHCHD10 activates OMA1 peptidase to disrupt mitochondrial cristae phenocopying patient mutations.

Liu YT, Huang X, Nguyen D, Shammas MK, Wu BP, Dombi E, Springer DA, Poulton J, Sekine S, Narendra DP
Human molecular genetics 2020 Jun 3;29(9):1547-1567

mTOR Overcomes Multiple Metabolic Restrictions to Enable HIV-1 Reverse Transcription and Intracellular Transport.

Taylor HE, Calantone N, Lichon D, Hudson H, Clerc I, Campbell EM, D'Aquila RT
Cell reports 2020 Jun 23;31(12):107810

Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.

Balsa E, Perry EA, Bennett CF, Jedrychowski M, Gygi SP, Doench JG, Puigserver P
Nature communications 2020 Jun 1;11(1):2714

The effect of MTHFD2 on the proliferation and migration of colorectal cancer cell lines.

Wei Y, Liu P, Li Q, Du J, Chen Y, Wang Y, Shi H, Wang Y, Zhang H, Xue W, Gao Y, Li D, Feng Y, Yan J, Han J, Zhang J
OncoTargets and therapy 2019;12:6361-6370

De novo synthesis of serine and glycine fuels purine nucleotide biosynthesis in human lung cancer tissues.

Fan TWM, Bruntz RC, Yang Y, Song H, Chernyavskaya Y, Deng P, Zhang Y, Shah PP, Beverly LJ, Qi Z, Mahan AL, Higashi RM, Dang CV, Lane AN
The Journal of biological chemistry 2019 Sep 6;294(36):13464-13477

Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation.

Wang LW, Shen H, Nobre L, Ersing I, Paulo JA, Trudeau S, Wang Z, Smith NA, Ma Y, Reinstadler B, Nomburg J, Sommermann T, Cahir-McFarland E, Gygi SP, Mootha VK, Weekes MP, Gewurz BE
Cell metabolism 2019 Sep 3;30(3):539-555.e11

Two separate functions of NME3 critical for cell survival underlie a neurodegenerative disorder.

Chen CW, Wang HL, Huang CW, Huang CY, Lim WK, Tu IC, Koorapati A, Hsieh ST, Kan HW, Tzeng SR, Liao JC, Chong WM, Naroditzky I, Kidron D, Eran A, Nijim Y, Sela E, Feldman HB, Kalfon L, Raveh-Barak H, Falik-Zaccai TC, Elpeleg O, Mandel H, Chang ZF
Proceedings of the National Academy of Sciences of the United States of America 2019 Jan 8;116(2):566-574

mTOR-S6K1 pathway mediates cytoophidium assembly.

Sun Z, Liu JL
Journal of genetics and genomics = Yi chuan xue bao 2019 Feb;46(2):65-74

Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.

Koufaris C, Nilsson R
Cancer & metabolism 2018;6:12

Histidine-Dependent Protein Methylation Is Required for Compartmentalization of CTP Synthase.

Lin WC, Chakraborty A, Huang SC, Wang PY, Hsieh YJ, Chien KY, Lee YH, Chang CC, Tang HY, Lin YT, Tung CS, Luo JD, Chen TW, Lin TY, Cheng ML, Chen YT, Yeh CT, Liu JL, Sung LY, Shiao MS, Yu JS, Chang YS, Pai LM
Cell reports 2018 Sep 4;24(10):2733-2745.e7

Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.

Dogan SA, Cerutti R, Benincá C, Brea-Calvo G, Jacobs HT, Zeviani M, Szibor M, Viscomi C
Cell metabolism 2018 Nov 6;28(5):764-775.e5

Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells.

Hitzel J, Lee E, Zhang Y, Bibli SI, Li X, Zukunft S, Pflüger B, Hu J, Schürmann C, Vasconez AE, Oo JA, Kratzer A, Kumar S, Rezende F, Josipovic I, Thomas D, Giral H, Schreiber Y, Geisslinger G, Fork C, Yang X, Sigala F, Romanoski CE, Kroll J, Jo H, Landmesser U, Lusis AJ, Namgaladze D, Fleming I, Leisegang MS, Zhu J, Brandes RP
Nature communications 2018 Jun 12;9(1):2292

mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.

Khan NA, Nikkanen J, Yatsuga S, Jackson C, Wang L, Pradhan S, Kivelä R, Pessia A, Velagapudi V, Suomalainen A
Cell metabolism 2017 Aug 1;26(2):419-428.e5

mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.

Ben-Sahra I, Hoxhaj G, Ricoult SJH, Asara JM, Manning BD
Science (New York, N.Y.) 2016 Feb 12;351(6274):728-733

Oxidative stress inhibits distant metastasis by human melanoma cells.

Piskounova E, Agathocleous M, Murphy MM, Hu Z, Huddlestun SE, Zhao Z, Leitch AM, Johnson TM, DeBerardinis RJ, Morrison SJ
Nature 2015 Nov 12;527(7577):186-91

Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus.

Sugiyama K, Ebinuma H, Nakamoto N, Sakasegawa N, Murakami Y, Chu PS, Usui S, Ishibashi Y, Wakayama Y, Taniki N, Murata H, Saito Y, Fukasawa M, Saito K, Yamagishi Y, Wakita T, Takaku H, Hibi T, Saito H, Kanai T
PloS one 2014;9(4):e94460

Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.

Mitsuishi Y, Taguchi K, Kawatani Y, Shibata T, Nukiwa T, Aburatani H, Yamamoto M, Motohashi H
Cancer cell 2012 Jul 10;22(1):66-79

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 12270-1-AP(MTHFD2 antibody) at dilution of 1:300
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The MTHFD2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MTHFD2. This antibody recognizes human, mouse, rat antigen. The MTHFD2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 12270-1-AP.pdf

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