LS-C185494

antibody from LSBio

Targeting: FASTK FAST

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C185494

Provider

LSBio

Product name

FASTK / FAST Antibody (aa346-549) LS-C185494

Antibody type

Polyclonal

Description

Protein A purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. FASTK / FAST antibody diluted at 1:1000.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

FAST antibody detects FASTK protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug A375 whole cell lysate/extract. 7.5 % SDS-PAGE. FAST antibody dilution:1:1000

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence of methanol-fixed HeLa using FAST antibody at 1:200 dilution.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence of methanol-fixed HeLa using FAST antibody at 1:200 dilution.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded SCC15 xenograft using FAST antibody at 1:100 dilution.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded SCC15 xenograft using FAST antibody at 1:100 dilution.