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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21449 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FASTK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, A375, NIH-3T3, PC-12.
- Concentration
- 6.56 mg/mL
Submitted references Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression.
Liu W, Lin YT, Yan XL, Ding YL, Wu YL, Chen WN, Lin X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Mar;29(3):1113-23
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Mar;29(3):1113-23
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FASTK Polyclonal Antibody (Product # PA5-21449). Sample (30 µg of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. FASTK Polyclonal Antibody (Product # PA5-21449) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FASTK Polyclonal Antibody detects FASTK protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract. E. 30 µg A375 whole cell lysate/extract.7.5 % SDS-PAGE. FASTK Polyclonal Antibody (Product # PA5-21449) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FASTK Polyclonal Antibody detects FASTK protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.7.5 % SDS-PAGE. FASTK Polyclonal Antibody (Product # PA5-21449) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FAST in methanol-fixed HeLa cells using a FAST polyclonal antibody (Product # PA5-21449) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using FAST antibody (Product # PA5-21449) at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FASTK Polyclonal Antibody detects FAST protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FAST protein stained by FASTK Polyclonal Antibody (Product # PA5-21449) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using FAST (Product # PA5-21449) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
