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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [5]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21449 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FASTK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, A375, NIH-3T3, PC-12. Predicted reactivity: Mouse (89%), Dog (95%), Pig (90%), Chimpanzee (99%), Bovine (88%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 6.56 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression.
Liu W, Lin YT, Yan XL, Ding YL, Wu YL, Chen WN, Lin X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Mar;29(3):1113-23
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Mar;29(3):1113-23
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FAST in methanol-fixed HeLa cells using a FAST polyclonal antibody (Product # PA5-21449) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using FAST antibody (Product # PA5-21449) at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FASTK Polyclonal Antibody detects FAST protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FAST protein stained by FASTK Polyclonal Antibody (Product # PA5-21449) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using FAST antibody (Product # PA5-21449) at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FASTK Polyclonal Antibody detects FAST protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FAST protein stained by FASTK Polyclonal Antibody (Product # PA5-21449) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using FAST (Product # PA5-21449) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
