H00007372-M05

antibody from Abnova Corporation

Targeting: UMPS

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007372-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007372-M05, RRID:AB_607274

Product name

UMPS monoclonal antibody (M05), clone 2F5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant UMPS.

Antigen sequence

DYTRAAVRMAEEHSEFVVGFISGSRVSMKPEFLHL
TPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVG
RGIISAADRLEAAEMYRKAAWEAYLSRLG


Isotype

IgG

Antibody clone number

2F5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Predictive and prognostic markers for the outcome of chemotherapy in advanced colorectal cancer, a retrospective analysis of the phase III randomised CAIRO study.

Koopman M, Venderbosch S, van Tinteren H, Ligtenberg MJ, Nagtegaal I, Van Krieken JH, Punt CJ
European journal of cancer (Oxford, England : 1990) 2009 Jul;45(11):1999-2006

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of UMPS expression in transfected 293T cell line by UMPS monoclonal antibody (M05), clone 2F5.Lane 1: UMPS transfected lysate(52.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged UMPS is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to UMPS on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol