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LearnH00023543-M01
antibody from Novus Biologicals
Targeting: RBFOX2
FOX-2, HNRBP2, HRNBP2, RBM9
Western blot ELISA Immunocytochemistry
Immunoprecipitation Immunohistochemistry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00023543-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00023543-M01, RRID:AB_2300945
- Product name
- Mouse Monoclonal FOX2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RBM9 - RNA binding motif protein 9
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references FOX-2 dependent splicing of ataxin-2 transcript is affected by ataxin-1 overexpression.
Welzel F, Kaehler C, Isau M, Hallen L, Lehrach H, Krobitsch S
PloS one 2012;7(5):e37985
PloS one 2012;7(5):e37985
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOX2 Antibody (4G3) [H00023543-M01] - Analysis of RBM9 over-expressed 293 cell line, cotransfected with RBM9 Validated Chimera RNAi ( Cat # H00023543-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RBM9 monoclonal antibody (M01), clone 4G3 (Cat # H00023543-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOX2 Antibody (4G3) [H00023543-M01] - Analysis of RBM9 expression in transfected 293T cell line by RBM9 monoclonal antibody (M01), clone 4G3.Lane 1: RBM9 transfected lysate(40.4 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOX2 Antibody (4G3) [H00023543-M01] - RBM9 monoclonal antibody (M01), clone 4G3 Analysis of RBM9 expression in NIH/3T3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: FOX2 Antibody (4G3) [H00023543-M01] - Detection limit for recombinant GST tagged RBM9 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: FOX2 Antibody (4G3) [H00023543-M01] - Analysis of monoclonal antibody to RBM9 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FOX2 Antibody (4G3) [H00023543-M01] - Analysis of monoclonal antibody to RBM9 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
