Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB110-40588 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-40588, RRID:AB_669966
- Product name
- Rabbit Polyclonal FOX2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references FOX-2 dependent splicing of ataxin-2 transcript is affected by ataxin-1 overexpression.
Genome-wide determination of a broad ESRP-regulated posttranscriptional network by high-throughput sequencing.
Autoregulation of Fox protein expression to produce dominant negative splicing factors.
MCAF1 and synergistic activation of the transcription of Epstein-Barr virus lytic genes by Rta and Zta.
Developmental control of CaV1.2 L-type calcium channel splicing by Fox proteins.
An RNA code for the FOX2 splicing regulator revealed by mapping RNA-protein interactions in stem cells.
Defining the regulatory network of the tissue-specific splicing factors Fox-1 and Fox-2.
Welzel F, Kaehler C, Isau M, Hallen L, Lehrach H, Krobitsch S
PloS one 2012;7(5):e37985
PloS one 2012;7(5):e37985
Genome-wide determination of a broad ESRP-regulated posttranscriptional network by high-throughput sequencing.
Dittmar KA, Jiang P, Park JW, Amirikian K, Wan J, Shen S, Xing Y, Carstens RP
Molecular and cellular biology 2012 Apr;32(8):1468-82
Molecular and cellular biology 2012 Apr;32(8):1468-82
Autoregulation of Fox protein expression to produce dominant negative splicing factors.
Damianov A, Black DL
RNA (New York, N.Y.) 2010 Feb;16(2):405-16
RNA (New York, N.Y.) 2010 Feb;16(2):405-16
MCAF1 and synergistic activation of the transcription of Epstein-Barr virus lytic genes by Rta and Zta.
Chang LK, Chuang JY, Nakao M, Liu ST
Nucleic acids research 2010 Aug;38(14):4687-700
Nucleic acids research 2010 Aug;38(14):4687-700
Developmental control of CaV1.2 L-type calcium channel splicing by Fox proteins.
Tang ZZ, Zheng S, Nikolic J, Black DL
Molecular and cellular biology 2009 Sep;29(17):4757-65
Molecular and cellular biology 2009 Sep;29(17):4757-65
An RNA code for the FOX2 splicing regulator revealed by mapping RNA-protein interactions in stem cells.
Yeo GW, Coufal NG, Liang TY, Peng GE, Fu XD, Gage FH
Nature structural & molecular biology 2009 Feb;16(2):130-7
Nature structural & molecular biology 2009 Feb;16(2):130-7
Defining the regulatory network of the tissue-specific splicing factors Fox-1 and Fox-2.
Zhang C, Zhang Z, Castle J, Sun S, Johnson J, Krainer AR, Zhang MQ
Genes & development 2008 Sep 15;22(18):2550-63
Genes & development 2008 Sep 15;22(18):2550-63
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOX2 Antibody [NB110-40588] - Detection of Human RBM9 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody NB110-40588 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOX2 Antibody [NB110-40588] - Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOX2 Antibody [NB110-40588] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB110-40588 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOX2 Antibody [NB110-40588] - Detection of mouse and rat RBM9 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody NB110-40588 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: FOX2 Antibody [NB110-40588] - Detection of human RBM9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody NB110-40588 (lot NB110-40588-2) used for IP at 6 ug/mg lysate. RBM9 was also immunoprecipitated by a previous lot (lot NB110-40588-1) of this antibody. For blotting immunoprecipitated RBM9, NB110-40588 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FOX2 Antibody [NB110-40588] - Sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 RBM9 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB