22115-1-AP

antibody from Proteintech Group

Targeting: INSIG1 CL-6, MGC1405

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

22115-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#22115-1-AP, RRID:AB_11183051

Product name

INSIG1 antibody

Antibody type

Polyclonal

Description

KD/KO validated INSIG1 antibody (Cat. #22115-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, WB,ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Tim-4 reprograms cholesterol metabolism to suppress antiviral innate immunity by disturbing the Insig1-SCAP interaction in macrophages.

Wang Y, Wang Y, Ding L, Ren X, Wang B, Wang L, Zhao S, Yue X, Wu Z, Li C, Liang X, Ma C, Gao L
Cell reports 2022 Nov 29;41(9):111738

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Renal tubule ectopic lipid deposition in diabetic kidney disease rat model and in vitro mechanism of leptin intervention.

Liu S, Da J, Yu J, Dong R, Yuan J, Yu F, Zha Y
Journal of physiology and biochemistry 2022 May;78(2):389-399

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Hypomorphic ASGR1 modulates lipid homeostasis via INSIG1-mediated SREBP signaling suppression.

Xu Y, Tao J, Yu X, Wu Y, Chen Y, You K, Zhang J, Getachew A, Pan T, Zhuang Y, Yuan F, Yang F, Lin X, Li YX
JCI insight 2021 Oct 8;6(19)

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The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Xu D, Wang Z, Xia Y, Shao F, Xia W, Wei Y, Li X, Qian X, Lee JH, Du L, Zheng Y, Lv G, Leu JS, Wang H, Xing D, Liang T, Hung MC, Lu Z
Nature 2020 Apr;580(7804):530-535

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Extracellular matrix mechanical cues regulate lipid metabolism through Lipin-1 and SREBP.

Romani P, Brian I, Santinon G, Pocaterra A, Audano M, Pedretti S, Mathieu S, Forcato M, Bicciato S, Manneville JB, Mitro N, Dupont S
Nature cell biology 2019 Mar;21(3):338-347

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(-)-Epicatechin regulates blood lipids and attenuates hepatic steatosis in rats fed high-fat diet.

Cheng H, Xu N, Zhao W, Su J, Liang M, Xie Z, Wu X, Li Q
Molecular nutrition & food research 2017 Nov;61(11)

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A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.

Cai HL, Tan QY, Jiang P, Dang RL, Xue Y, Tang MM, Xu P, Deng Y, Li HD, Yao JK
Translational psychiatry 2015 Oct 20;5(10):e661

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Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: involvement of the INSIG/SREBP pathway.

Dang R, Jiang P, Cai H, Li H, Guo R, Wu Y, Zhang L, Zhu W, He X, Liu Y, Xu P
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 2015 Aug;25(8):1239-47

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP(INSIG1 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The INSIG1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human INSIG1. This antibody recognizes human, mouse antigen. The INSIG1 antibody has been validated for the following applications: ELISA, WB, IF, IHC analysis.

Appendix

22115-1-AP.pdf

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Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 22115-1-AP(INSIG1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue