53-3219-41
antibody from Invitrogen Antibodies
Targeting: F11R
CD321, JAM-1, JAM-A, JAM1, JAMA, JCAM, PAM-1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- 53-3219-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD321 (F11R) Monoclonal Antibody (CSTEM27), Alexa Fluor 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This monoclonal antibody CSTEM27 reacts with human CD321 (F11R, JAM-1, JAM-A). Applications Reported: This CSTEM27 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis. This CSTEM27 antibody has been tested by immunocytochemistry of adherent cells. This can be used at less than or equal to 5 µg/mL. This CSTEM27 antibody has also been tested by flow cytometric analysis of human iPSCs. This may be used at 0.25 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- CSTEM27
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized human iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2a K Isotype Control Alexa Fluor® 488 (Product # 53-4724-80) (left) or 5 µg/mL Anti-CD321 (F11R) Alexa Fluor® 488 (right). Nuclei are stained with DAPI. An iPSC colony (positive staining) and murine derived feeder cells (negative staining) are in both fields of view above.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of a mixture of human iPSC and the C2C12 murine cell line with Anti-Human TRA-1-60 PerCp-eFluor 710 (Product # 46-8863-82) and Mouse IgG2a K Isotype Control Alexa Fluor® 488 (Product # 53-4724-80) (left) or Anti-Human CD321 (F11R) Alexa Fluor® 488 (right).
- Conjugate
- Green dye