710228

antibody from Invitrogen Antibodies

Targeting: F11R CD321, JAM-1, JAM-A, JAM1, JAMA, JCAM, PAM-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

710228

Provider

Invitrogen Antibodies

Product name

JAM-A (CD321) Recombinant Polyclonal Antibody (24HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

24HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of JAM1 in whole cell extracts of serum-starved MCF-7 cells treated with IGF1 (150 ng/mL, 15 min) using a JAM1 Recombinant Rabbit Polyclonal Antibody (Product # 710228) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~33 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of JAM1/CD321 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with JAM1 (24HCLC) Recombinant Rabbit Polyclonal Antibody (Product # 710228) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cell junctional localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.