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- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF1103 - Provider product page
- Provider
- R&D Systems
- Product name
- Human JAM-A Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human JAM-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse JAM-A is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y624- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references miR-143 and miR-145 disrupt the cervical epithelial barrier through dysregulation of cell adhesion, apoptosis and proliferation.
JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals.
Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C ε-mediated zona occludens-1 phosphorylation at threonine 770/772.
Mesenchymal stem cells transmigrate between and directly through tumor necrosis factor-α-activated endothelial cells via both leukocyte-like and novel mechanisms.
Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium.
The F11 receptor (F11R/JAM-A) in atherothrombosis: overexpression of F11R in atherosclerotic plaques.
Junctional adhesion molecule 1 is a functional receptor for feline calicivirus.
Anton L, DeVine A, Sierra LJ, Brown AG, Elovitz MA
Scientific reports 2017 Jun 8;7(1):3020
Scientific reports 2017 Jun 8;7(1):3020
JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals.
Williams DW, Anastos K, Morgello S, Berman JW
Journal of leukocyte biology 2015 Feb;97(2):401-12
Journal of leukocyte biology 2015 Feb;97(2):401-12
Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C ε-mediated zona occludens-1 phosphorylation at threonine 770/772.
Chattopadhyay R, Dyukova E, Singh NK, Ohba M, Mobley JA, Rao GN
The Journal of biological chemistry 2014 Feb 7;289(6):3148-63
The Journal of biological chemistry 2014 Feb 7;289(6):3148-63
Mesenchymal stem cells transmigrate between and directly through tumor necrosis factor-α-activated endothelial cells via both leukocyte-like and novel mechanisms.
Teo GS, Ankrum JA, Martinelli R, Boetto SE, Simms K, Sciuto TE, Dvorak AM, Karp JM, Carman CV
Stem cells (Dayton, Ohio) 2012 Nov;30(11):2472-86
Stem cells (Dayton, Ohio) 2012 Nov;30(11):2472-86
Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
Haarmann A, Deiss A, Prochaska J, Foerch C, Weksler B, Romero I, Couraud PO, Stoll G, Rieckmann P, Buttmann M
PloS one 2010 Oct 21;5(10):e13568
PloS one 2010 Oct 21;5(10):e13568
Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium.
Economopoulou M, Hammer J, Wang F, Fariss R, Maminishkis A, Miller SS
Investigative ophthalmology & visual science 2009 Mar;50(3):1454-63
Investigative ophthalmology & visual science 2009 Mar;50(3):1454-63
The F11 receptor (F11R/JAM-A) in atherothrombosis: overexpression of F11R in atherosclerotic plaques.
Babinska A, Azari BM, Salifu MO, Liu R, Jiang XC, Sobocka MB, Boo D, Al Khoury G, Deitch JS, Marmur JD, Ehrlich YH, Kornecki E
Thrombosis and haemostasis 2007 Feb;97(2):272-81
Thrombosis and haemostasis 2007 Feb;97(2):272-81
Junctional adhesion molecule 1 is a functional receptor for feline calicivirus.
Makino A, Shimojima M, Miyazawa T, Kato K, Tohya Y, Akashi H
Journal of virology 2006 May;80(9):4482-90
Journal of virology 2006 May;80(9):4482-90
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- JAM-A in MCF-7 Human Cell Line. JAM-A was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human JAM-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1103) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to intercellular junctions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
