TA811840S

antibody from Invitrogen Antibodies

Targeting: PCSK9 FH3, HCHOLA3, NARC-1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA811840S

Provider

Invitrogen Antibodies

Product name

PCSK9 Monoclonal Antibody (OTI8E10), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI8E10

Vial size

30 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCSK9 (RC220000, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PCSK9. Positive lysates LY403563 (100 µg) and LC403563 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Equivalent amounts of cell lysates (10 µg per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and PCSK9-Knockout Hela cells (KO, Cat# LC810408) were separated by SDS-PAGE and immunoblotted with anti-PCSK9 monoclonal antibody TA811840. Then the blotted membrane was stripped and reprobed with anti-beta-actin antibody (TA811000) as a loading control. (1:500)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (35 µg) from 2 different cell lines by using anti-PCSK9 monoclonal antibody. (1:500)