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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AP13520PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP13520PU-N, RRID:AB_1748940
- Product name
- anti Aurora kinase C (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of Human Aurora-C.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (Lane 1), Flag-tagged Aurora A (Lane 2), Flag-tagged Aurora B (Lane 3) or Flag-tagged Aurora C (Lane 4). In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Aurora kinase C Antibody (C-term) Cat.-No AP13520PU in HepG2 cell line lysates (35ug/lane). This demonstrates the Aurora-C antibody detected the Aurora-C protein (arrow).
