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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-29902 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP2J2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Huh7, HepG2.
- Concentration
- 0.34 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYP2J2 using 30 µg of A) A431 and B) H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CYP2J2 polyclonal antibody (Product # PA5-29902) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CYP2J2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CYP2J2 Polyclonal Antibody (Product # PA5-29902) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using CYP2J2 (Product # PA5-29902) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
