Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-24328 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- N2DL4 Monoclonal Antibody (709116)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, approximately 20% cross-reactivity with recombinant human (rh) ULBP-2 is observed and no cross-reactivity with rhULBP-1 or rhULBP-3 is observed. Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 709116
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HT-29 human colon adenocarcinoma cell line and Caco-2 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of mouse Anti-human ULBP-4/RAET1E Monoclonal Antibody (Product # MA5-24328) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for ULBP-4/RAET1E at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HT-29 human colon adenocarcinoma cell line and Caco-2 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of mouse Anti-human ULBP-4/RAET1E Monoclonal Antibody (Product # MA5-24328) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for ULBP-4/RAET1E at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ULBP-4/RAET1E was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using mouse Anti-human ULBP-4/RAET1E Monoclonal Antibody (Product # MA5-24328) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-mouse HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 human hepatocellular carcinoma cell line was stained with mouse Anti-human ULBP-4/RAET1E Monoclonal Antibody (Product # MA5-24328, filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-mouse IgG Secondary Antibody.