Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 600-401-865 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-865, RRID:AB_2211023
- Product name
- Anti-Human UBC12 (RABBIT) Antibody - 600-401-865
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression.
Alphavirus production is inhibited in neurofibromin 1-deficient cells through activated RAS signalling.
Liu X, Reitsma JM, Mamrosh JL, Zhang Y, Straube R, Deshaies RJ
Molecular cell 2018 Mar 1;69(5):773-786.e6
Molecular cell 2018 Mar 1;69(5):773-786.e6
Alphavirus production is inhibited in neurofibromin 1-deficient cells through activated RAS signalling.
Kolokoltsova OA, Domina AM, Kolokoltsov AA, Davey RA, Weaver SC, Watowich SJ
Virology 2008 Jul 20;377(1):133-42
Virology 2008 Jul 20;377(1):133-42
No comments: Submit comment
Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot analysis is shown using Rockland's Affinity Purified anti-UBC12 antibody to detect Human UBC12 in various preparations. This western blot shows reactivity with purified human UBC12 protein (lane 1), NIH-3T3 cells over expressing UBC12 by infection (lane 2) and endogenous UBC12 in NIH 3T3 cells (lane 3). Peptide competition (lane 4) blocks specific reactivity of the antibody with purified UBC12 protein. Comparison to a molecular weight marker (not shown) indicates a single band of ~21.0 kDa corresponding to the expected molecular weight for the protein. The blot was blocked with 5% non-fat dry milk in TBS supplemented with 0.1% Tween-20 at 4° C overnight. After washes the blot was incubated with a 1:1,000 dilution of the antibody at room temperature for 2 h in TBS-Tween. Washes consisted of 3 changes of TBS-Tween buffer for 15 min each. Detection occurred using HRP anti-Rabbit IgG diluted 1:2,000 and signal processing by chemiluminescence reagent with a 10-sec exposure time. Other detection systems will yield similar results. Personal communication Martine Roussel.
- Validation comment
- Western Blot
