600-401-254

antibody from Rockland Immunochemicals, Inc.

Targeting: ALB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-254

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-254, RRID:AB_2226071

Product name

Anti-MOUSE SERUM ALBUMIN (RABBIT) Antibody - 600-401-254

Antibody type

Polyclonal

Vial size

1 µl

References

Submitted references

Immune Monitoring of Trans-endothelial Transport by Kidney-Resident Macrophages.

Stamatiades EG, Tremblay ME, Bohm M, Crozet L, Bisht K, Kao D, Coelho C, Fan X, Yewdell WT, Davidson A, Heeger PS, Diebold S, Nimmerjahn F, Geissmann F
Cell 2016 Aug 11;166(4):991-1003

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of rabbit anti-mouse serum albumin. Lane 1: molecular weight. Lane 2: mouse serum albumin. Load: 0.1 µg. Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:20,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) 30 min at 4°C. Predicted/Observed size: 69 kDa, 72 kDa for MSA. Other band(s): none.

Validation comment

Western Blot