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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- Ab114897 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant MEKK2 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human MEKK2 Antibody, Recombinant, could be used for WB, IF, ICC and so on.Application:WB: 1/500-1/1000IF/ICC: 1/50-1/200 Protein FunctionComponent of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.Post-translationalAutophosphorylated.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 11-27- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,6 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant MEKK2 Antibody (Ab114897) - Western Blot All lanes: Recombinant MEKK2 Antibody (Ab114897) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 70 kDa Observed band size: 75 kDa Exposure time: 6.3 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant MEKK2 Antibody (Ab114897) - IF/ICC Immunocytochemistry analysis of MEKK2 (green) in HeLa using Recombinant MEKK2 Antibody (Ab114897), and cells were counterstained with DAPI (blue).
