Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB7128 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat MEKK2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects recombinant human MEKK2 in direct ELISA and human, mouse, rat MEKK2 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MEKK1, 3, or 4 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y2U5
- Isotype
- IgG
- Antibody clone number
- 722728
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat MEKK2 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Saos-2 human osteosarcoma cell line, BJAB human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat MEKK2 Monoclonal Antibody (Catalog # MAB7128) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MEKK2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MEKK2 in 293T Human Cell Line. MEKK2 was detected in immersion fixed 293T human embryonic kidney cell line using Mouse Anti-Human/Mouse/Rat MEKK2 Monoclonal Antibody (Catalog # MAB7128) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This application has not been tested in mouse or rat samples.