Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AF7416 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal RBBP4/RbAp48 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse and rat RBBP4 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human RBBP7 is observed. Detects recombinant human RBBP4 in direct ELISAs. In direct ELISAs, approximately 75% cross-reactivity with recombinant human RBBP7 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat RBBP4 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, Neuro-2A mouse neuroblastoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RBBP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7416) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RBBP4 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.