M02702-1

antibody from Boster Biological Technology

Targeting: RBBP4 lin-53, NURF55, RbAp48

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M02702-1

Provider

Boster Biological Technology

Product name

Anti-RbAp48 RBBP4 Antibody Picoband™ (monoclonal, 9F3)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for RbAp48 detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

9F3

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquoted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of RbAp48 using anti-RbAp48 antibody (M02702-1). RbAp48 was detected in paraffin-embedded section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-RbAp48 Antibody (M02702-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Additional image