Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A010-12AP - Provider product page
- Provider
- Badrilla Ltd
- Product name
- Phospholamban Phospho Serine-16 (AP) IgG
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Phospholamban (PLB/PLN) is a small transmembrane protein which plays an important role in controlling the activity of the sarcoplasmic reticulum ATPase (SERCA2a) of cardiac muscle during calcium sequestration. Phospholamban is phosphorylated on separate amino acid residues by cAMP-dependent, and cGMP-dependent (Ser-16) and Ca2+/CaM-dependent (Thr-17) protein kinases in response to β-adrenergic stimulation. Akt has also been shown to phosphorylate Thr-17. The result is an increased calcium pump activity which reduces the time course of the calcium transient, increases the calcium load in the sarcoplasmic reticulum, and consequently, produces a larger calcium transient at the next action potential. However, alteration in this homeostatic interaction has been shown to result in heart failure
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Hamster, Rabbit, Sheep
- Host
- Rabbit
- Antigen sequence
R9SAIRRAS(PO3H2)TIE19Y conjugated t
o KLH- Isotype
- IgG
- Vial size
- 50 µl
- Storage
- Lyophilised: 4 °C Reconstituted:-80 °C
- Handling
- Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles
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Supportive validation
- Submitted by
- Badrilla Ltd (provider)
- Main image
- Experimental details
- Western blot
- Sample type
- Canine sarcoplasmic reticulum +/- PKA treatment and rat cardiac myocytes +/- isoprenaline treatment (not boiled)
- Validation comment
- Ab stains for PLB monomer and multimer in PKA treated samples, but not unphosphorylated
- Primary Ab dilution
- 3.513888889
- Secondary Ab dilution
- 1.430555556
- Protocol
- Protocol