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- Antibody Data
- Antigen structure
- References [176]
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- Western blot [1]
- Immunocytochemistry [1]
- Other assay [47]
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- Product number
- MA3-922 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospholamban Monoclonal Antibody (2D12)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA3-922 detects phospholamban (PLB) in rabbit, human, pig, sheep, chicken, canine, guinea porcine, rat, and mouse. MA3-922 has been successfully used in Western blot, immunofluorescence, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 25 kDa protein under non-reducing conditions representing the pentameric form of PLB and a 5 kDa protein under reducing conditions representing the monomeric form of PLB in canine cardiac extracts. Immunofluorescence staining of PLB in rat papillary muscle tissue with MA3-922 results in diffuse staining of the network sarcoplasmic reticulum (SR). MA3-922 can also be used to increase the calcium uptake in ventricular SR vesicles analogous to the phosphorylation of PLB following adrenergic stimulation. This antibody is recommended for immunofluorescent staining of tissues. The MA3-922 immunogen is a synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine PLB. This antibody recognizes an epitope between amino acid residues 9-17 of canine PLB.
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Guinea Pig, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2D12
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Physiological calcium combined with electrical pacing accelerates maturation of human engineered heart tissue.
Interleukin-6-Mediated-Ca(2+) Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.
Cohesin-protein Shugoshin-1 controls cardiac automaticity via HCN4 pacemaker channel.
Breast Cancer Promotes Cardiac Dysfunction Through Deregulation of Cardiomyocyte Ca(2+)-Handling Protein Expression That is Not Reversed by Exercise Training.
Phospholamban antisense oligonucleotides improve cardiac function in murine cardiomyopathy.
Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells.
Cold-Inducible RNA-Binding Protein Prevents an Excessive Heart Rate Response to Stress by Targeting Phosphodiesterase.
The phospholamban p.(Arg14del) pathogenic variant leads to cardiomyopathy with heart failure and is unreponsive to standard heart failure therapy.
Long-term effects of empagliflozin on excitation-contraction-coupling in human induced pluripotent stem cell cardiomyocytes.
Phosphorylation of cardiac myosin-binding protein-C contributes to calcium homeostasis.
Altered calcium handling produces reentry-promoting action potential alternans in atrial fibrillation-remodeled hearts.
Nanoscale reorganization of sarcoplasmic reticulum in pressure-overload cardiac hypertrophy visualized by dSTORM.
Differential localizations of protein phosphatase 1 isoforms determine their physiological function in the heart.
Characterization of biventricular alterations in myocardial (reverse) remodelling in aortic banding-induced chronic pressure overload.
Saxagliptin but Not Sitagliptin Inhibits CaMKII and PKC via DPP9 Inhibition in Cardiomyocytes.
Regulation of BECN1-mediated autophagy by HSPB6: Insights from a human HSPB6(S10F) mutant.
Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure.
The DWORF micropeptide enhances contractility and prevents heart failure in a mouse model of dilated cardiomyopathy.
Empagliflozin Ammeliorates High Glucose Induced-Cardiac Dysfuntion in Human iPSC-Derived Cardiomyocytes.
Effects of hypoestrogenism and/or hyperaldosteronism on myocardial remodeling in female mice.
Three-dimensional imaging reveals endo(sarco)plasmic reticulum-containing invaginations within the nucleoplasm of muscle.
Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice.
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity.
High-fat diet induces protein kinase A and G-protein receptor kinase phosphorylation of β(2) -adrenergic receptor and impairs cardiac adrenergic reserve in animal hearts.
Up-regulation of Intracellular Calcium Handling Underlies the Recovery of Endotoxemic Cardiomyopathy in Mice.
Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice.
Loss of type 9 adenylyl cyclase triggers reduced phosphorylation of Hsp20 and diastolic dysfunction.
Heart failure modulates electropharmacological characteristics of sinoatrial nodes.
Increased passive stiffness promotes diastolic dysfunction despite improved Ca2+ handling during left ventricular concentric hypertrophy.
Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure.
TRPC4α and TRPC4β Similarly Affect Neonatal Cardiomyocyte Survival during Chronic GPCR Stimulation.
Genetically Encoded Biosensors Reveal PKA Hyperphosphorylation on the Myofilaments in Rabbit Heart Failure.
Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers.
Sex-specific cardiovascular responses to control or high fat diet feeding in C57bl/6 mice chronically exposed to bisphenol A.
Sex Hormones Promote Opposite Effects on ACE and ACE2 Activity, Hypertrophy and Cardiac Contractility in Spontaneously Hypertensive Rats.
Atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins.
Loss of Rad-GTPase produces a novel adaptive cardiac phenotype resistant to systolic decline with aging.
Interplay between the E2F pathway and β-adrenergic signaling in the pathological hypertrophic response of myocardium.
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype.
Chronic lead exposure increases blood pressure and myocardial contractility in rats.
Chronic exposure to low doses of HgCl2 avoids calcium handling impairment in the right ventricle after myocardial infarction in rats.
Regional ion channel gene expression heterogeneity and ventricular fibrillation dynamics in human hearts.
The beneficial effects of ranolazine on cardiac function after myocardial infarction are greater in diabetic than in nondiabetic rats.
Integrin-linked kinase mediates force transduction in cardiomyocytes by modulating SERCA2a/PLN function.
Ryanodine receptor-mediated calcium leak drives progressive development of an atrial fibrillation substrate in a transgenic mouse model.
Obesity does not lead to imbalance between myocardial phospholamban phosphorylation and dephosphorylation.
Progesterone modulates SERCA2a expression and function in rabbit cardiomyocytes.
Measuring Ca2+-dependent Ca2+-uptake activity in the mouse heart.
Co-expression of SERCA isoforms, phospholamban and sarcolipin in human skeletal muscle fibers.
Carvedilol prevents ovariectomy-induced myocardial contractile dysfunction in female rat.
Aldosterone blockade reduces mortality without changing cardiac remodeling in spontaneously hypertensive rats.
Cardiac structure/function, protein expression, and DNA methylation are changed in adult female mice exposed to diethylstilbestrol in utero.
Identifying cellular mechanisms of zinc-induced relaxation in isolated cardiomyocytes.
SERCA Cys674 sulphonylation and inhibition of L-type Ca2+ influx contribute to cardiac dysfunction in endotoxemic mice, independent of cGMP synthesis.
Lifelong exposure to bisphenol a alters cardiac structure/function, protein expression, and DNA methylation in adult mice.
Inhibition of thyroid hormone receptor α1 impairs post-ischemic cardiac performance after myocardial infarction in mice.
Right ventricular pacing with mechanical dyssynchrony causes apoptosis interruptus and calcium mishandling.
Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?
Targeted sarcoplasmic reticulum Ca2+ ATPase 2a gene delivery to restore electrical stability in the failing heart.
Estradiol promotes sudden cardiac death in transgenic long QT type 2 rabbits while progesterone is protective.
Cardiac response to doxorubicin and dexrazoxane in intact and ovariectomized young female rats at rest and after swim training.
Functional scaffold-free 3-D cardiac microtissues: a novel model for the investigation of heart cells.
Targeted deletion of microRNA-22 promotes stress-induced cardiac dilation and contractile dysfunction.
Sildenafil prevents and reverses transverse-tubule remodeling and Ca(2+) handling dysfunction in right ventricle failure induced by pulmonary artery hypertension.
Fosinopril attenuates the doxorubicin-induced cardiomyopathy by restoring the function of sarcoplasmic reticulum.
Decrease in the density of t-tubular L-type Ca2+ channel currents in failing ventricular myocytes.
Prevention of ventricular arrhythmia and calcium dysregulation in a catecholaminergic polymorphic ventricular tachycardia mouse model carrying calsequestrin-2 mutation.
Angiotensin receptor blockade improves the net balance of cardiac Ca(2+) handling-related proteins in sympathetic hyperactivity-induced heart failure.
Baroreflex sensitivity impairment is associated with cardiac diastolic dysfunction in rats.
Silencing calcineurin A subunit reduces SERCA2 expression in cardiac myocytes.
cAMP- and Ca²(+) /calmodulin-dependent protein kinases mediate inotropic, lusitropic and arrhythmogenic effects of urocortin 2 in mouse ventricular myocytes.
Slowed relaxation and preserved maximal force in soleus muscles of mice with targeted disruption of the Serca2 gene in skeletal muscle.
Hyperglycemia can delay left ventricular dysfunction but not autonomic damage after myocardial infarction in rodents.
Myocardial contractility is preserved early but reduced late after ovariectomy in young female rats.
cGMP signals modulate cAMP levels in a compartment-specific manner to regulate catecholamine-dependent signaling in cardiac myocytes.
Cerebral ischemia elicits aberration in myocardium contractile function and intracellular calcium handling.
Caspase-dependent protein phosphatase 2A activation contributes to endotoxin-induced cardiomyocyte contractile dysfunction.
NADPH oxidase inhibition ameliorates cardiac dysfunction in rabbits with heart failure.
Superior calcium homeostasis of extraocular muscles.
Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy.
Role of CaMKIIdelta phosphorylation of the cardiac ryanodine receptor in the force frequency relationship and heart failure.
Succinate modulates Ca(2+) transient and cardiomyocyte viability through PKA-dependent pathway.
Thyroid hormone can favorably remodel the diabetic myocardium after acute myocardial infarction.
More severe cellular phenotype in human idiopathic dilated cardiomyopathy compared to ischemic heart disease.
Ryanodine receptor phosphorylation by calcium/calmodulin-dependent protein kinase II promotes life-threatening ventricular arrhythmias in mice with heart failure.
Effects of thapsigargin and phenylephrine on calcineurin and protein kinase C signaling functions in cardiac myocytes.
Electrophysiological consequences of dyssynchronous heart failure and its restoration by resynchronization therapy.
Increased Ca2+ sensitivity of the ryanodine receptor mutant RyR2R4496C underlies catecholaminergic polymorphic ventricular tachycardia.
Induced overexpression of Na+/Ca2+ exchanger transgene: altered myocyte contractility, [Ca2+]i transients, SR Ca2+ contents, and action potential duration.
Exercise training during diabetes attenuates cardiac ryanodine receptor dysregulation.
Nonionic detergent phase extraction for the proteomic analysis of heart membrane proteins using label-free LC-MS.
Impaired cardiac contractility in mice lacking both the AE3 Cl-/HCO3- exchanger and the NKCC1 Na+-K+-2Cl- cotransporter: effects on Ca2+ handling and protein phosphatases.
Enhanced calcium cycling and contractile function in transgenic hearts expressing constitutively active G alpha o* protein.
Unexpected structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin: a complex arrhythmogenic cascade in a knock in mouse model.
Serotonin increases L-type Ca2+ current and SR Ca2+ content through 5-HT4 receptors in failing rat ventricular cardiomyocytes.
Cellular and molecular determinants of altered Ca2+ handling in the failing rabbit heart: primary defects in SR Ca2+ uptake and release mechanisms.
Cellular trafficking of phospholamban and formation of functional sarcoplasmic reticulum during myocyte differentiation.
Myosin regulatory light chain E22K mutation results in decreased cardiac intracellular calcium and force transients.
Thyroid hormone attenuates cardiac remodeling and improves hemodynamics early after acute myocardial infarction in rats.
UCP1-independent thermogenesis in white adipose tissue of cold-acclimated Ucp1-/- mice.
Functional properties of human embryonic stem cell-derived cardiomyocytes: intracellular Ca2+ handling and the role of sarcoplasmic reticulum in the contraction.
Identification of a novel phosphorylation site in protein phosphatase inhibitor-1 as a negative regulator of cardiac function.
Increased cardiac adenylyl cyclase expression is associated with increased survival after myocardial infarction.
Differential activation of stress-response signaling in load-induced cardiac hypertrophy and failure.
Chronic ventricular myocyte-specific overexpression of angiotensin II type 2 receptor results in intrinsic myocyte contractile dysfunction.
Genetic manipulation of calcium-handling proteins in cardiac myocytes. I. Experimental studies.
Protein kinase C and preconditioning: role of the sarcoplasmic reticulum.
Impaired Ca2+ handling and contraction in cardiomyocytes from mice with a dominant negative thyroid hormone receptor alpha1.
Phosphorylation-status of phospholamban and calsequestrin modifies their affinity towards commonly used antibodies.
Adenylyl cyclase type VI gene transfer reduces phospholamban expression in cardiac myocytes via activating transcription factor 3.
Doxycycline inducible expression of SERCA2a improves calcium handling and reverts cardiac dysfunction in pressure overload-induced cardiac hypertrophy.
The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm.
Alterations in myofilament function contribute to left ventricular dysfunction in pigs early after myocardial infarction.
Improved expression and characterization of Ca2+-ATPase and phospholamban in High-Five cells.
Accelerated onset of heart failure in mice during pressure overload with chronically decreased SERCA2 calcium pump activity.
Frequency- and afterload-dependent cardiac modulation in vivo by troponin I with constitutively active protein kinase A phosphorylation sites.
Insights into cardioprotection obtained from study of cellular Ca2+ handling in myocardium of true hibernating mammals.
Sarcoplasmic reticulum calcium defect in Ras-induced hypertrophic cardiomyopathy heart.
Mechanical unloading improves intracellular Ca2+ regulation in rats with doxorubicin-induced cardiomyopathy.
Autologous stem cell transplantation for myocardial repair.
S100A1 gene transfer: a strategy to strengthen engineered cardiac grafts.
Ca2+/calmodulin-dependent protein kinase II phosphorylation regulates the cardiac ryanodine receptor.
Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation.
Mechanism of enhanced cardiac function in mice with hypertrophy induced by overexpressed Akt.
Targeted inhibition of Ca2+/calmodulin-dependent protein kinase II in cardiac longitudinal sarcoplasmic reticulum results in decreased phospholamban phosphorylation at threonine 17.
Effect of tachycardia on myocardial sarcoplasmic reticulum and Ca2+ dynamics: a mechanism for preconditioning?
Effect of tachycardia on myocardial sarcoplasmic reticulum and Ca2+ dynamics: a mechanism for preconditioning?
Gender differences in sarcoplasmic reticulum calcium loading after isoproterenol.
Regional alterations in protein expression in the dyssynchronous failing heart.
Altered dose response to beta-agonists in SERCA1a-expressing hearts ex vivo and in vivo.
Defective intracellular Ca(2+) signaling contributes to cardiomyopathy in Type 1 diabetic rats.
Abnormal Ca2+ release, but normal ryanodine receptors, in canine and human heart failure.
Type 1 phosphatase, a negative regulator of cardiac function.
Type 1 phosphatase, a negative regulator of cardiac function.
Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart.
Ca(2+) signaling in cardiac myocytes overexpressing the alpha(1) subunit of L-type Ca(2+) channel.
Ca(2+) signaling in cardiac myocytes overexpressing the alpha(1) subunit of L-type Ca(2+) channel.
Targeting phospholamban by gene transfer in human heart failure.
Targeting phospholamban by gene transfer in human heart failure.
Interaction between increased SERCA2a activity and beta -adrenoceptor stimulation in adult rabbit myocytes.
Cardiac remodeling and atrial fibrillation in transgenic mice overexpressing junctin.
Developmental changes of Ca(2+) handling in mouse ventricular cells from early embryo to adulthood.
Developmental changes of Ca(2+) handling in mouse ventricular cells from early embryo to adulthood.
Overexpression of the sarcoplasmic reticulum Ca(2+)-ATPase improves myocardial contractility in diabetic cardiomyopathy.
Overexpression of the sarcoplasmic reticulum Ca(2+)-ATPase improves myocardial contractility in diabetic cardiomyopathy.
Altered SR protein expression associated with contractile dysfunction in diabetic rat hearts.
mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope.
mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope.
S100A1: a regulator of myocardial contractility.
Impaired sarcoplasmic reticulum function leads to contractile dysfunction and cardiac hypertrophy.
Interactions between phospholamban and beta-adrenergic drive may lead to cardiomyopathy and early mortality.
Interactions between phospholamban and beta-adrenergic drive may lead to cardiomyopathy and early mortality.
Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes.
Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers.
Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers.
Sprint training normalizes Ca(2+) transients and SR function in postinfarction rat myocytes.
Sprint training normalizes Ca(2+) transients and SR function in postinfarction rat myocytes.
Cardiac-specific overexpression of a superinhibitory pentameric phospholamban mutant enhances inhibition of cardiac function in vivo.
Mechanisms of altered excitation-contraction coupling in canine tachycardia-induced heart failure, I: experimental studies.
Mechanisms of altered excitation-contraction coupling in canine tachycardia-induced heart failure, I: experimental studies.
Nitric oxide synthase in cardiac sarcoplasmic reticulum.
Nitric oxide synthase in cardiac sarcoplasmic reticulum.
In vivo phosphorylation of cardiac troponin I by protein kinase Cbeta2 decreases cardiomyocyte calcium responsiveness and contractility in transgenic mouse hearts.
Regulation of Ca2+ handling by phosphorylation status in mouse fast- and slow-twitch skeletal muscle fibers.
Purified, reconstituted cardiac Ca2+-ATPase is regulated by phospholamban but not by direct phosphorylation with Ca2+/calmodulin-dependent protein kinase.
Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching.
Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching.
Ca(2+)-transporting ATPase, phospholamban, and calsequestrin levels in nonfailing and failing human myocardium.
Ca(2+)-transporting ATPase, phospholamban, and calsequestrin levels in nonfailing and failing human myocardium.
Residues 2-25 of phospholamban are insufficient to inhibit Ca2+ transport ATPase of cardiac sarcoplasmic reticulum.
The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle.
The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle.
Comparative studies of cardiac and skeletal sarcoplasmic reticulum ATPases. Effect of a phospholamban antibody on enzyme activation by Ca2+.
Phospholamban expressed in slow-twitch and chronically stimulated fast-twitch muscles minimally affects calcium affinity of sarcoplasmic reticulum Ca(2+)-ATPase.
Phospholamban mediates the beta-adrenergic-enhanced Ca2+ uptake in mammalian ventricular myocytes.
Phospholamban mediates the beta-adrenergic-enhanced Ca2+ uptake in mammalian ventricular myocytes.
Shen S, Sewanan LR, Shao S, Halder SS, Stankey P, Li X, Campbell SG
Stem cell reports 2022 Sep 13;17(9):2037-2049
Stem cell reports 2022 Sep 13;17(9):2037-2049
Interleukin-6-Mediated-Ca(2+) Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.
Liao J, Zhang S, Yang S, Lu Y, Lu K, Wu Y, Wu Q, Zhao N, Dong Q, Chen L, Du Y
Frontiers in immunology 2021;12:758157
Frontiers in immunology 2021;12:758157
Cohesin-protein Shugoshin-1 controls cardiac automaticity via HCN4 pacemaker channel.
Liu D, Song AT, Qi X, van Vliet PP, Xiao J, Xiong F, Andelfinger G, Nattel S
Nature communications 2021 May 5;12(1):2551
Nature communications 2021 May 5;12(1):2551
Breast Cancer Promotes Cardiac Dysfunction Through Deregulation of Cardiomyocyte Ca(2+)-Handling Protein Expression That is Not Reversed by Exercise Training.
da Costa TSR, Urias U, Negrao MV, Jordão CP, Passos CS, Gomes-Santos IL, Salemi VMC, Camargo AA, Brum PC, Oliveira EM, Hajjar LA, Chammas R, Filho RK, Negrao CE
Journal of the American Heart Association 2021 Feb;10(5):e018076
Journal of the American Heart Association 2021 Feb;10(5):e018076
Phospholamban antisense oligonucleotides improve cardiac function in murine cardiomyopathy.
Grote Beverborg N, Später D, Knöll R, Hidalgo A, Yeh ST, Elbeck Z, Silljé HHW, Eijgenraam TR, Siga H, Zurek M, Palmér M, Pehrsson S, Albery T, Bomer N, Hoes MF, Boogerd CJ, Frisk M, van Rooij E, Damle S, Louch WE, Wang QD, Fritsche-Danielson R, Chien KR, Hansson KM, Mullick AE, de Boer RA, van der Meer P
Nature communications 2021 Aug 30;12(1):5180
Nature communications 2021 Aug 30;12(1):5180
Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells.
Liang W, Han P, Kim EH, Mak J, Zhang R, Torrente AG, Goldhaber JI, Marbán E, Cho HC
Stem cells (Dayton, Ohio) 2020 Mar;38(3):352-368
Stem cells (Dayton, Ohio) 2020 Mar;38(3):352-368
Cold-Inducible RNA-Binding Protein Prevents an Excessive Heart Rate Response to Stress by Targeting Phosphodiesterase.
Xie D, Geng L, Xiong K, Zhao T, Wang S, Xue J, Wang C, Wang G, Feng Z, Zhou H, Li Y, Li L, Liu Y, Xue Z, Yang J, Ma H, Liang D, Chen YH
Circulation research 2020 Jun 5;126(12):1706-1720
Circulation research 2020 Jun 5;126(12):1706-1720
The phospholamban p.(Arg14del) pathogenic variant leads to cardiomyopathy with heart failure and is unreponsive to standard heart failure therapy.
Eijgenraam TR, Boukens BJ, Boogerd CJ, Schouten EM, van de Kolk CWA, Stege NM, Te Rijdt WP, Hoorntje ET, van der Zwaag PA, van Rooij E, van Tintelen JP, van den Berg MP, van der Meer P, van der Velden J, Silljé HHW, de Boer RA
Scientific reports 2020 Jun 17;10(1):9819
Scientific reports 2020 Jun 17;10(1):9819
Long-term effects of empagliflozin on excitation-contraction-coupling in human induced pluripotent stem cell cardiomyocytes.
Pabel S, Reetz F, Dybkova N, Shomroni O, Salinas G, Mustroph J, Hammer KP, Hasenfuss G, Hamdani N, Maier LS, Streckfuss-Bömeke K, Sossalla S
Journal of molecular medicine (Berlin, Germany) 2020 Dec;98(12):1689-1700
Journal of molecular medicine (Berlin, Germany) 2020 Dec;98(12):1689-1700
Phosphorylation of cardiac myosin-binding protein-C contributes to calcium homeostasis.
Kumar M, Haghighi K, Kranias EG, Sadayappan S
The Journal of biological chemistry 2020 Aug 7;295(32):11275-11291
The Journal of biological chemistry 2020 Aug 7;295(32):11275-11291
Altered calcium handling produces reentry-promoting action potential alternans in atrial fibrillation-remodeled hearts.
Liu T, Xiong F, Qi XY, Xiao J, Villeneuve L, Abu-Taha I, Dobrev D, Huang C, Nattel S
JCI insight 2020 Apr 7;5(8)
JCI insight 2020 Apr 7;5(8)
Nanoscale reorganization of sarcoplasmic reticulum in pressure-overload cardiac hypertrophy visualized by dSTORM.
Hadipour-Lakmehsari S, Driouchi A, Lee SH, Kuzmanov U, Callaghan NI, Heximer SP, Simmons CA, Yip CM, Gramolini AO
Scientific reports 2019 May 27;9(1):7867
Scientific reports 2019 May 27;9(1):7867
Differential localizations of protein phosphatase 1 isoforms determine their physiological function in the heart.
Liu R, Miller C, D'Annibale C, Vo K, Jacobs A
Acta biochimica et biophysica Sinica 2019 Mar 1;51(3):323-330
Acta biochimica et biophysica Sinica 2019 Mar 1;51(3):323-330
Characterization of biventricular alterations in myocardial (reverse) remodelling in aortic banding-induced chronic pressure overload.
Miranda-Silva D, Gonçalves-Rodrigues P, Almeida-Coelho J, Hamdani N, Lima T, Conceição G, Sousa-Mendes C, Cláudia-Moura, González A, Díez J, Linke WA, Leite-Moreira A, Falcão-Pires I
Scientific reports 2019 Feb 27;9(1):2956
Scientific reports 2019 Feb 27;9(1):2956
Saxagliptin but Not Sitagliptin Inhibits CaMKII and PKC via DPP9 Inhibition in Cardiomyocytes.
Koyani CN, Trummer C, Shrestha N, Scheruebel S, Bourgeois B, Plastira I, Kickmaier S, Sourij H, Rainer PP, Madl T, Sattler W, Pelzmann B, Malle E, von Lewinski D
Frontiers in physiology 2018;9:1622
Frontiers in physiology 2018;9:1622
Regulation of BECN1-mediated autophagy by HSPB6: Insights from a human HSPB6(S10F) mutant.
Liu GS, Zhu H, Cai WF, Wang X, Jiang M, Essandoh K, Vafiadaki E, Haghighi K, Lam CK, Gardner G, Adly G, Nicolaou P, Sanoudou D, Liang Q, Rubinstein J, Fan GC, Kranias EG
Autophagy 2018;14(1):80-97
Autophagy 2018;14(1):80-97
Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure.
Kushnir A, Santulli G, Reiken SR, Coromilas E, Godfrey SJ, Brunjes DL, Colombo PC, Yuzefpolskaya M, Sokol SI, Kitsis RN, Marks AR
Circulation 2018 Sep 11;138(11):1144-1154
Circulation 2018 Sep 11;138(11):1144-1154
The DWORF micropeptide enhances contractility and prevents heart failure in a mouse model of dilated cardiomyopathy.
Makarewich CA, Munir AZ, Schiattarella GG, Bezprozvannaya S, Raguimova ON, Cho EE, Vidal AH, Robia SL, Bassel-Duby R, Olson EN
eLife 2018 Oct 9;7
eLife 2018 Oct 9;7
Empagliflozin Ammeliorates High Glucose Induced-Cardiac Dysfuntion in Human iPSC-Derived Cardiomyocytes.
Ng KM, Lau YM, Dhandhania V, Cai ZJ, Lee YK, Lai WH, Tse HF, Siu CW
Scientific reports 2018 Oct 5;8(1):14872
Scientific reports 2018 Oct 5;8(1):14872
Effects of hypoestrogenism and/or hyperaldosteronism on myocardial remodeling in female mice.
Rouet-Benzineb P, Merval R, Polidano E
Physiological reports 2018 Nov;6(21):e13912
Physiological reports 2018 Nov;6(21):e13912
Three-dimensional imaging reveals endo(sarco)plasmic reticulum-containing invaginations within the nucleoplasm of muscle.
Lee SH, Hadipour-Lakmehsari S, Miyake T, Gramolini AO
American journal of physiology. Cell physiology 2018 Mar 1;314(3):C257-C267
American journal of physiology. Cell physiology 2018 Mar 1;314(3):C257-C267
Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice.
Fajardo VA, Gamu D, Mitchell A, Bloemberg D, Bombardier E, Chambers PJ, Bellissimo C, Quadrilatero J, Tupling AR
PloS one 2017;12(3):e0173708
PloS one 2017;12(3):e0173708
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity.
Jacobsen BB, Leopoldo APL, Cordeiro JP, Campos DHS, Nascimento AFD, Sugizaki MM, Cicogna AC, Padovani CR, Leopoldo AS
Arquivos brasileiros de cardiologia 2017 Nov;109(5):432-439
Arquivos brasileiros de cardiologia 2017 Nov;109(5):432-439
High-fat diet induces protein kinase A and G-protein receptor kinase phosphorylation of β(2) -adrenergic receptor and impairs cardiac adrenergic reserve in animal hearts.
Fu Q, Hu Y, Wang Q, Liu Y, Li N, Xu B, Kim S, Chiamvimonvat N, Xiang YK
The Journal of physiology 2017 Mar 15;595(6):1973-1986
The Journal of physiology 2017 Mar 15;595(6):1973-1986
Up-regulation of Intracellular Calcium Handling Underlies the Recovery of Endotoxemic Cardiomyopathy in Mice.
Morse JC, Huang J, Khona N, Miller EJ, Siwik DA, Colucci WS, Hobai IA
Anesthesiology 2017 Jun;126(6):1125-1138
Anesthesiology 2017 Jun;126(6):1125-1138
Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice.
Ravel-Chapuis A, Bélanger G, Côté J, Michel RN, Jasmin BJ
Human molecular genetics 2017 Jun 15;26(12):2192-2206
Human molecular genetics 2017 Jun 15;26(12):2192-2206
Loss of type 9 adenylyl cyclase triggers reduced phosphorylation of Hsp20 and diastolic dysfunction.
Li Y, Baldwin TA, Wang Y, Subramaniam J, Carbajal AG, Brand CS, Cunha SR, Dessauer CW
Scientific reports 2017 Jul 17;7(1):5522
Scientific reports 2017 Jul 17;7(1):5522
Heart failure modulates electropharmacological characteristics of sinoatrial nodes.
Chang SL, Chuang HL, Chen YC, Kao YH, Lin YK, Yeh YH, Chen SA, Chen YJ
Experimental and therapeutic medicine 2017 Feb;13(2):771-779
Experimental and therapeutic medicine 2017 Feb;13(2):771-779
Increased passive stiffness promotes diastolic dysfunction despite improved Ca2+ handling during left ventricular concentric hypertrophy.
Røe ÅT, Aronsen JM, Skårdal K, Hamdani N, Linke WA, Danielsen HE, Sejersted OM, Sjaastad I, Louch WE
Cardiovascular research 2017 Aug 1;113(10):1161-1172
Cardiovascular research 2017 Aug 1;113(10):1161-1172
Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure.
Sadredini M, Danielsen TK, Aronsen JM, Manotheepan R, Hougen K, Sjaastad I, Stokke MK
PloS one 2016;11(4):e0153887
PloS one 2016;11(4):e0153887
TRPC4α and TRPC4β Similarly Affect Neonatal Cardiomyocyte Survival during Chronic GPCR Stimulation.
Kirschmer N, Bandleon S, von Ehrlich-Treuenstätt V, Hartmann S, Schaaf A, Lamprecht AK, Miranda-Laferte E, Langsenlehner T, Ritter O, Eder P
PloS one 2016;11(12):e0168446
PloS one 2016;11(12):e0168446
Genetically Encoded Biosensors Reveal PKA Hyperphosphorylation on the Myofilaments in Rabbit Heart Failure.
Barbagallo F, Xu B, Reddy GR, West T, Wang Q, Fu Q, Li M, Shi Q, Ginsburg KS, Ferrier W, Isidori AM, Naro F, Patel HH, Bossuyt J, Bers D, Xiang YK
Circulation research 2016 Sep 30;119(8):931-43
Circulation research 2016 Sep 30;119(8):931-43
Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers.
Fajardo VA, Smith IC, Bombardier E, Chambers PJ, Quadrilatero J, Tupling AR
Brain and behavior 2016 Jun;6(6):e00470
Brain and behavior 2016 Jun;6(6):e00470
Sex-specific cardiovascular responses to control or high fat diet feeding in C57bl/6 mice chronically exposed to bisphenol A.
Patel BB, Raad M, Sebag IA, Chalifour LE
Toxicology reports 2015;2:1310-1318
Toxicology reports 2015;2:1310-1318
Sex Hormones Promote Opposite Effects on ACE and ACE2 Activity, Hypertrophy and Cardiac Contractility in Spontaneously Hypertensive Rats.
Dalpiaz PL, Lamas AZ, Caliman IF, Ribeiro RF Jr, Abreu GR, Moyses MR, Andrade TU, Gouvea SA, Alves MF, Carmona AK, Bissoli NS
PloS one 2015;10(5):e0127515
PloS one 2015;10(5):e0127515
Atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins.
Lugenbiel P, Wenz F, Govorov K, Schweizer PA, Katus HA, Thomas D
PloS one 2015;10(3):e0116395
PloS one 2015;10(3):e0116395
Loss of Rad-GTPase produces a novel adaptive cardiac phenotype resistant to systolic decline with aging.
Manning JR, Withers CN, Levitan B, Smith JD, Andres DA, Satin J
American journal of physiology. Heart and circulatory physiology 2015 Oct;309(8):H1336-45
American journal of physiology. Heart and circulatory physiology 2015 Oct;309(8):H1336-45
Interplay between the E2F pathway and β-adrenergic signaling in the pathological hypertrophic response of myocardium.
Major JL, Salih M, Tuana BS
Journal of molecular and cellular cardiology 2015 Jul;84:179-90
Journal of molecular and cellular cardiology 2015 Jul;84:179-90
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype.
Fajardo VA, Bombardier E, McMillan E, Tran K, Wadsworth BJ, Gamu D, Hopf A, Vigna C, Smith IC, Bellissimo C, Michel RN, Tarnopolsky MA, Quadrilatero J, Tupling AR
Disease models & mechanisms 2015 Aug 1;8(8):999-1009
Disease models & mechanisms 2015 Aug 1;8(8):999-1009
Chronic lead exposure increases blood pressure and myocardial contractility in rats.
Fioresi M, Simões MR, Furieri LB, Broseghini-Filho GB, Vescovi MV, Stefanon I, Vassallo DV
PloS one 2014;9(5):e96900
PloS one 2014;9(5):e96900
Chronic exposure to low doses of HgCl2 avoids calcium handling impairment in the right ventricle after myocardial infarction in rats.
Faria Tde O, Costa GP, Almenara CC, Angeli JK, Vassallo DV, Stefanon I, Vassallo PF
PloS one 2014;9(4):e95639
PloS one 2014;9(4):e95639
Regional ion channel gene expression heterogeneity and ventricular fibrillation dynamics in human hearts.
Sivagangabalan G, Nazzari H, Bignolais O, Maguy A, Naud P, Farid T, Massé S, Gaborit N, Varro A, Nair K, Backx P, Vigmond E, Nattel S, Demolombe S, Nanthakumar K
PloS one 2014;9(1):e82179
PloS one 2014;9(1):e82179
The beneficial effects of ranolazine on cardiac function after myocardial infarction are greater in diabetic than in nondiabetic rats.
Mourouzis I, Mantzouratou P, Galanopoulos G, Kostakou E, Dhalla AK, Belardinelli L, Pantos C
Journal of cardiovascular pharmacology and therapeutics 2014 Sep;19(5):457-69
Journal of cardiovascular pharmacology and therapeutics 2014 Sep;19(5):457-69
Integrin-linked kinase mediates force transduction in cardiomyocytes by modulating SERCA2a/PLN function.
Traister A, Li M, Aafaqi S, Lu M, Arab S, Radisic M, Gross G, Guido F, Sherret J, Verma S, Slorach C, Mertens L, Hui W, Roy A, Delgado-Olguín P, Hannigan G, Maynes JT, Coles JG
Nature communications 2014 Sep 11;5:4533
Nature communications 2014 Sep 11;5:4533
Ryanodine receptor-mediated calcium leak drives progressive development of an atrial fibrillation substrate in a transgenic mouse model.
Li N, Chiang DY, Wang S, Wang Q, Sun L, Voigt N, Respress JL, Ather S, Skapura DG, Jordan VK, Horrigan FT, Schmitz W, Müller FU, Valderrabano M, Nattel S, Dobrev D, Wehrens XHT
Circulation 2014 Mar 25;129(12):1276-1285
Circulation 2014 Mar 25;129(12):1276-1285
Obesity does not lead to imbalance between myocardial phospholamban phosphorylation and dephosphorylation.
Freire PP, Alves CA, Deus AF, Leopoldo AP, Leopoldo AS, Silva DC, Tomasi LC, Campos DH, Cicogna AC
Arquivos brasileiros de cardiologia 2014 Jul;103(1):41-50
Arquivos brasileiros de cardiologia 2014 Jul;103(1):41-50
Progesterone modulates SERCA2a expression and function in rabbit cardiomyocytes.
Moshal KS, Zhang Z, Roder K, Kim TY, Cooper L, Patedakis Litvinov B, Lu Y, Reddy V, Terentyev D, Choi BR, Koren G
American journal of physiology. Cell physiology 2014 Dec 1;307(11):C1050-7
American journal of physiology. Cell physiology 2014 Dec 1;307(11):C1050-7
Measuring Ca2+-dependent Ca2+-uptake activity in the mouse heart.
Holemans T, Vandecaetsbeek I, Wuytack F, Vangheluwe P
Cold Spring Harbor protocols 2014 Aug 1;2014(8):876-86
Cold Spring Harbor protocols 2014 Aug 1;2014(8):876-86
Co-expression of SERCA isoforms, phospholamban and sarcolipin in human skeletal muscle fibers.
Fajardo VA, Bombardier E, Vigna C, Devji T, Bloemberg D, Gamu D, Gramolini AO, Quadrilatero J, Tupling AR
PloS one 2013;8(12):e84304
PloS one 2013;8(12):e84304
Carvedilol prevents ovariectomy-induced myocardial contractile dysfunction in female rat.
Ribeiro RF Jr, Potratz FF, Pavan BM, Forechi L, Lima FL, Fiorim J, Fernandes AA, Vassallo DV, Stefanon I
PloS one 2013;8(1):e53226
PloS one 2013;8(1):e53226
Aldosterone blockade reduces mortality without changing cardiac remodeling in spontaneously hypertensive rats.
Cezar MD, Damatto RL, Martinez PF, Lima AR, Campos DH, Rosa CM, Guizoni DM, Bonomo C, Cicogna AC, Gimenes R, Pagan LU, Okoshi MP, Okoshi K
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2013;32(5):1275-87
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2013;32(5):1275-87
Cardiac structure/function, protein expression, and DNA methylation are changed in adult female mice exposed to diethylstilbestrol in utero.
Haddad R, Kasneci A, Sebag IA, Chalifour LE
Canadian journal of physiology and pharmacology 2013 Sep;91(9):741-9
Canadian journal of physiology and pharmacology 2013 Sep;91(9):741-9
Identifying cellular mechanisms of zinc-induced relaxation in isolated cardiomyocytes.
Yi T, Vick JS, Vecchio MJ, Begin KJ, Bell SP, Delay RJ, Palmer BM
American journal of physiology. Heart and circulatory physiology 2013 Sep 1;305(5):H706-15
American journal of physiology. Heart and circulatory physiology 2013 Sep 1;305(5):H706-15
SERCA Cys674 sulphonylation and inhibition of L-type Ca2+ influx contribute to cardiac dysfunction in endotoxemic mice, independent of cGMP synthesis.
Hobai IA, Buys ES, Morse JC, Edgecomb J, Weiss EH, Armoundas AA, Hou X, Khandelwal AR, Siwik DA, Brouckaert P, Cohen RA, Colucci WS
American journal of physiology. Heart and circulatory physiology 2013 Oct 15;305(8):H1189-200
American journal of physiology. Heart and circulatory physiology 2013 Oct 15;305(8):H1189-200
Lifelong exposure to bisphenol a alters cardiac structure/function, protein expression, and DNA methylation in adult mice.
Patel BB, Raad M, Sebag IA, Chalifour LE
Toxicological sciences : an official journal of the Society of Toxicology 2013 May;133(1):174-85
Toxicological sciences : an official journal of the Society of Toxicology 2013 May;133(1):174-85
Inhibition of thyroid hormone receptor α1 impairs post-ischemic cardiac performance after myocardial infarction in mice.
Mourouzis I, Kostakou E, Galanopoulos G, Mantzouratou P, Pantos C
Molecular and cellular biochemistry 2013 Jul;379(1-2):97-105
Molecular and cellular biochemistry 2013 Jul;379(1-2):97-105
Right ventricular pacing with mechanical dyssynchrony causes apoptosis interruptus and calcium mishandling.
Klug D, Boule S, Wissocque L, Montaigne D, Marechal X, Hassoun SM, Neviere R
The Canadian journal of cardiology 2013 Apr;29(4):510-8
The Canadian journal of cardiology 2013 Apr;29(4):510-8
Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?
Middlekauff HR, Vigna C, Verity MA, Fonarow GC, Horwich TB, Hamilton MA, Shieh P, Tupling AR
Journal of cardiac failure 2012 Sep;18(9):724-33
Journal of cardiac failure 2012 Sep;18(9):724-33
Targeted sarcoplasmic reticulum Ca2+ ATPase 2a gene delivery to restore electrical stability in the failing heart.
Cutler MJ, Wan X, Plummer BN, Liu H, Deschenes I, Laurita KR, Hajjar RJ, Rosenbaum DS
Circulation 2012 Oct 23;126(17):2095-104
Circulation 2012 Oct 23;126(17):2095-104
Estradiol promotes sudden cardiac death in transgenic long QT type 2 rabbits while progesterone is protective.
Odening KE, Choi BR, Liu GX, Hartmann K, Ziv O, Chaves L, Schofield L, Centracchio J, Zehender M, Peng X, Brunner M, Koren G
Heart rhythm 2012 May;9(5):823-32
Heart rhythm 2012 May;9(5):823-32
Cardiac response to doxorubicin and dexrazoxane in intact and ovariectomized young female rats at rest and after swim training.
Calvé A, Haddad R, Barama SN, Meilleur M, Sebag IA, Chalifour LE
American journal of physiology. Heart and circulatory physiology 2012 May 15;302(10):H2048-57
American journal of physiology. Heart and circulatory physiology 2012 May 15;302(10):H2048-57
Functional scaffold-free 3-D cardiac microtissues: a novel model for the investigation of heart cells.
Desroches BR, Zhang P, Choi BR, King ME, Maldonado AE, Li W, Rago A, Liu G, Nath N, Hartmann KM, Yang B, Koren G, Morgan JR, Mende U
American journal of physiology. Heart and circulatory physiology 2012 May 15;302(10):H2031-42
American journal of physiology. Heart and circulatory physiology 2012 May 15;302(10):H2031-42
Targeted deletion of microRNA-22 promotes stress-induced cardiac dilation and contractile dysfunction.
Gurha P, Abreu-Goodger C, Wang T, Ramirez MO, Drumond AL, van Dongen S, Chen Y, Bartonicek N, Enright AJ, Lee B, Kelm RJ Jr, Reddy AK, Taffet GE, Bradley A, Wehrens XH, Entman ML, Rodriguez A
Circulation 2012 Jun 5;125(22):2751-61
Circulation 2012 Jun 5;125(22):2751-61
Sildenafil prevents and reverses transverse-tubule remodeling and Ca(2+) handling dysfunction in right ventricle failure induced by pulmonary artery hypertension.
Xie YP, Chen B, Sanders P, Guo A, Li Y, Zimmerman K, Wang LC, Weiss RM, Grumbach IM, Anderson ME, Song LS
Hypertension (Dallas, Tex. : 1979) 2012 Feb;59(2):355-62
Hypertension (Dallas, Tex. : 1979) 2012 Feb;59(2):355-62
Fosinopril attenuates the doxorubicin-induced cardiomyopathy by restoring the function of sarcoplasmic reticulum.
Zhang YC, Tang Y, Zhang M, Chen J, Zhou Q, Sun YG, Chen MT, Xu WP
Cell biochemistry and biophysics 2012 Dec;64(3):205-11
Cell biochemistry and biophysics 2012 Dec;64(3):205-11
Decrease in the density of t-tubular L-type Ca2+ channel currents in failing ventricular myocytes.
Horiuchi-Hirose M, Kashihara T, Nakada T, Kurebayashi N, Shimojo H, Shibazaki T, Sheng X, Yano S, Hirose M, Hongo M, Sakurai T, Moriizumi T, Ueda H, Yamada M
American journal of physiology. Heart and circulatory physiology 2011 Mar;300(3):H978-88
American journal of physiology. Heart and circulatory physiology 2011 Mar;300(3):H978-88
Prevention of ventricular arrhythmia and calcium dysregulation in a catecholaminergic polymorphic ventricular tachycardia mouse model carrying calsequestrin-2 mutation.
Alcalai R, Wakimoto H, Arad M, Planer D, Konno T, Wang L, Seidman JG, Seidman CE, Berul CI
Journal of cardiovascular electrophysiology 2011 Mar;22(3):316-24
Journal of cardiovascular electrophysiology 2011 Mar;22(3):316-24
Angiotensin receptor blockade improves the net balance of cardiac Ca(2+) handling-related proteins in sympathetic hyperactivity-induced heart failure.
Ferreira JC, Moreira JB, Campos JC, Pereira MG, Mattos KC, Coelho MA, Brum PC
Life sciences 2011 Mar 28;88(13-14):578-85
Life sciences 2011 Mar 28;88(13-14):578-85
Baroreflex sensitivity impairment is associated with cardiac diastolic dysfunction in rats.
Mostarda C, Moraes-Silva IC, Moreira ED, Medeiros A, Piratello AC, Consolim-Colombo FM, Caldini EG, Brum PC, Krieger EM, Irigoyen MC
Journal of cardiac failure 2011 Jun;17(6):519-25
Journal of cardiac failure 2011 Jun;17(6):519-25
Silencing calcineurin A subunit reduces SERCA2 expression in cardiac myocytes.
Prasad AM, Inesi G
American journal of physiology. Heart and circulatory physiology 2011 Jan;300(1):H173-80
American journal of physiology. Heart and circulatory physiology 2011 Jan;300(1):H173-80
cAMP- and Ca²(+) /calmodulin-dependent protein kinases mediate inotropic, lusitropic and arrhythmogenic effects of urocortin 2 in mouse ventricular myocytes.
Yang LZ, Kockskämper J, Khan S, Suarez J, Walther S, Doleschal B, Unterer G, Khafaga M, Mächler H, Heinzel FR, Dillmann WH, Pieske B, Spiess J
British journal of pharmacology 2011 Jan;162(2):544-56
British journal of pharmacology 2011 Jan;162(2):544-56
Slowed relaxation and preserved maximal force in soleus muscles of mice with targeted disruption of the Serca2 gene in skeletal muscle.
Sjåland C, Lunde PK, Swift F, Munkvik M, Ericsson M, Lunde M, Boye S, Christensen G, Ellingsen Ø, Sejersted OM, Andersson KB
The Journal of physiology 2011 Dec 15;589(Pt 24):6139-55
The Journal of physiology 2011 Dec 15;589(Pt 24):6139-55
Hyperglycemia can delay left ventricular dysfunction but not autonomic damage after myocardial infarction in rodents.
Rodrigues B, Rosa KT, Medeiros A, Schaan BD, Brum PC, De Angelis K, Irigoyen MC
Cardiovascular diabetology 2011 Apr 6;10:26
Cardiovascular diabetology 2011 Apr 6;10:26
Myocardial contractility is preserved early but reduced late after ovariectomy in young female rats.
Paigel AS, Ribeiro RF Jr, Fernandes AA, Targueta GP, Vassallo DV, Stefanon I
Reproductive biology and endocrinology : RB&E 2011 Apr 23;9:54
Reproductive biology and endocrinology : RB&E 2011 Apr 23;9:54
cGMP signals modulate cAMP levels in a compartment-specific manner to regulate catecholamine-dependent signaling in cardiac myocytes.
Stangherlin A, Gesellchen F, Zoccarato A, Terrin A, Fields LA, Berrera M, Surdo NC, Craig MA, Smith G, Hamilton G, Zaccolo M
Circulation research 2011 Apr 15;108(8):929-39
Circulation research 2011 Apr 15;108(8):929-39
Cerebral ischemia elicits aberration in myocardium contractile function and intracellular calcium handling.
Sun L, Ai J, Wang N, Zhang R, Li J, Zhang T, Wu W, Hang P, Lu Y, Yang B
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2010;26(3):421-30
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2010;26(3):421-30
Caspase-dependent protein phosphatase 2A activation contributes to endotoxin-induced cardiomyocyte contractile dysfunction.
Neviere R, Hassoun SM, Decoster B, Bouazza Y, Montaigne D, Maréchal X, Marciniak C, Marchetti P, Lancel S
Critical care medicine 2010 Oct;38(10):2031-6
Critical care medicine 2010 Oct;38(10):2031-6
NADPH oxidase inhibition ameliorates cardiac dysfunction in rabbits with heart failure.
Liu Y, Huang H, Xia W, Tang Y, Li H, Huang C
Molecular and cellular biochemistry 2010 Oct;343(1-2):143-53
Molecular and cellular biochemistry 2010 Oct;343(1-2):143-53
Superior calcium homeostasis of extraocular muscles.
Zeiger U, Mitchell CH, Khurana TS
Experimental eye research 2010 Nov;91(5):613-22
Experimental eye research 2010 Nov;91(5):613-22
Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy.
Di Blasi C, Blasevich F, Bellafiore E, Mottarelli E, Gibertini S, Zanotti S, Saredi S, Mantegazza R, Morandi L, Mora M
Neuromuscular disorders : NMD 2010 May;20(5):326-9
Neuromuscular disorders : NMD 2010 May;20(5):326-9
Role of CaMKIIdelta phosphorylation of the cardiac ryanodine receptor in the force frequency relationship and heart failure.
Kushnir A, Shan J, Betzenhauser MJ, Reiken S, Marks AR
Proceedings of the National Academy of Sciences of the United States of America 2010 Jun 1;107(22):10274-9
Proceedings of the National Academy of Sciences of the United States of America 2010 Jun 1;107(22):10274-9
Succinate modulates Ca(2+) transient and cardiomyocyte viability through PKA-dependent pathway.
Aguiar CJ, Andrade VL, Gomes ER, Alves MN, Ladeira MS, Pinheiro AC, Gomes DA, Almeida AP, Goes AM, Resende RR, Guatimosim S, Leite MF
Cell calcium 2010 Jan;47(1):37-46
Cell calcium 2010 Jan;47(1):37-46
Thyroid hormone can favorably remodel the diabetic myocardium after acute myocardial infarction.
Kalofoutis C, Mourouzis I, Galanopoulos G, Dimopoulos A, Perimenis P, Spanou D, Cokkinos DV, Singh J, Pantos C
Molecular and cellular biochemistry 2010 Dec;345(1-2):161-9
Molecular and cellular biochemistry 2010 Dec;345(1-2):161-9
More severe cellular phenotype in human idiopathic dilated cardiomyopathy compared to ischemic heart disease.
Hamdani N, Borbély A, Veenstra SP, Kooij V, Vrydag W, Zaremba R, Dos Remedios C, Niessen HW, Michel MC, Paulus WJ, Stienen GJ, van der Velden J
Journal of muscle research and cell motility 2010 Dec;31(4):289-301
Journal of muscle research and cell motility 2010 Dec;31(4):289-301
Ryanodine receptor phosphorylation by calcium/calmodulin-dependent protein kinase II promotes life-threatening ventricular arrhythmias in mice with heart failure.
van Oort RJ, McCauley MD, Dixit SS, Pereira L, Yang Y, Respress JL, Wang Q, De Almeida AC, Skapura DG, Anderson ME, Bers DM, Wehrens XH
Circulation 2010 Dec 21;122(25):2669-79
Circulation 2010 Dec 21;122(25):2669-79
Effects of thapsigargin and phenylephrine on calcineurin and protein kinase C signaling functions in cardiac myocytes.
Prasad AM, Inesi G
American journal of physiology. Cell physiology 2009 May;296(5):C992-C1002
American journal of physiology. Cell physiology 2009 May;296(5):C992-C1002
Electrophysiological consequences of dyssynchronous heart failure and its restoration by resynchronization therapy.
Aiba T, Hesketh GG, Barth AS, Liu T, Daya S, Chakir K, Dimaano VL, Abraham TP, O'Rourke B, Akar FG, Kass DA, Tomaselli GF
Circulation 2009 Mar 10;119(9):1220-30
Circulation 2009 Mar 10;119(9):1220-30
Increased Ca2+ sensitivity of the ryanodine receptor mutant RyR2R4496C underlies catecholaminergic polymorphic ventricular tachycardia.
Fernández-Velasco M, Rueda A, Rizzi N, Benitah JP, Colombi B, Napolitano C, Priori SG, Richard S, Gómez AM
Circulation research 2009 Jan 30;104(2):201-9, 12p following 209
Circulation research 2009 Jan 30;104(2):201-9, 12p following 209
Induced overexpression of Na+/Ca2+ exchanger transgene: altered myocyte contractility, [Ca2+]i transients, SR Ca2+ contents, and action potential duration.
Wang J, Chan TO, Zhang XQ, Gao E, Song J, Koch WJ, Feldman AM, Cheung JY
American journal of physiology. Heart and circulatory physiology 2009 Aug;297(2):H590-601
American journal of physiology. Heart and circulatory physiology 2009 Aug;297(2):H590-601
Exercise training during diabetes attenuates cardiac ryanodine receptor dysregulation.
Shao CH, Wehrens XH, Wyatt TA, Parbhu S, Rozanski GJ, Patel KP, Bidasee KR
Journal of applied physiology (Bethesda, Md. : 1985) 2009 Apr;106(4):1280-92
Journal of applied physiology (Bethesda, Md. : 1985) 2009 Apr;106(4):1280-92
Nonionic detergent phase extraction for the proteomic analysis of heart membrane proteins using label-free LC-MS.
Donoghue PM, Hughes C, Vissers JP, Langridge JI, Dunn MJ
Proteomics 2008 Sep;8(18):3895-905
Proteomics 2008 Sep;8(18):3895-905
Impaired cardiac contractility in mice lacking both the AE3 Cl-/HCO3- exchanger and the NKCC1 Na+-K+-2Cl- cotransporter: effects on Ca2+ handling and protein phosphatases.
Prasad V, Bodi I, Meyer JW, Wang Y, Ashraf M, Engle SJ, Doetschman T, Sisco K, Nieman ML, Miller ML, Lorenz JN, Shull GE
The Journal of biological chemistry 2008 Nov 14;283(46):31303-14
The Journal of biological chemistry 2008 Nov 14;283(46):31303-14
Enhanced calcium cycling and contractile function in transgenic hearts expressing constitutively active G alpha o* protein.
Zhu M, Gach AA, Liu G, Xu X, Lim CC, Zhang JX, Mao L, Chuprun K, Koch WJ, Liao R, Koren G, Blaxall BC, Mende U
American journal of physiology. Heart and circulatory physiology 2008 Mar;294(3):H1335-47
American journal of physiology. Heart and circulatory physiology 2008 Mar;294(3):H1335-47
Unexpected structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin: a complex arrhythmogenic cascade in a knock in mouse model.
Rizzi N, Liu N, Napolitano C, Nori A, Turcato F, Colombi B, Bicciato S, Arcelli D, Spedito A, Scelsi M, Villani L, Esposito G, Boncompagni S, Protasi F, Volpe P, Priori SG
Circulation research 2008 Aug 1;103(3):298-306
Circulation research 2008 Aug 1;103(3):298-306
Serotonin increases L-type Ca2+ current and SR Ca2+ content through 5-HT4 receptors in failing rat ventricular cardiomyocytes.
Birkeland JA, Swift F, Tovsrud N, Enger U, Lunde PK, Qvigstad E, Levy FO, Sejersted OM, Sjaastad I
American journal of physiology. Heart and circulatory physiology 2007 Oct;293(4):H2367-76
American journal of physiology. Heart and circulatory physiology 2007 Oct;293(4):H2367-76
Cellular and molecular determinants of altered Ca2+ handling in the failing rabbit heart: primary defects in SR Ca2+ uptake and release mechanisms.
Armoundas AA, Rose J, Aggarwal R, Stuyvers BD, O'rourke B, Kass DA, Marbán E, Shorofsky SR, Tomaselli GF, William Balke C
American journal of physiology. Heart and circulatory physiology 2007 Mar;292(3):H1607-18
American journal of physiology. Heart and circulatory physiology 2007 Mar;292(3):H1607-18
Cellular trafficking of phospholamban and formation of functional sarcoplasmic reticulum during myocyte differentiation.
Stenoien DL, Knyushko TV, Londono MP, Opresko LK, Mayer MU, Brady ST, Squier TC, Bigelow DJ
American journal of physiology. Cell physiology 2007 Jun;292(6):C2084-94
American journal of physiology. Cell physiology 2007 Jun;292(6):C2084-94
Myosin regulatory light chain E22K mutation results in decreased cardiac intracellular calcium and force transients.
Szczesna-Cordary D, Jones M, Moore JR, Watt J, Kerrick WG, Xu Y, Wang Y, Wagg C, Lopaschuk GD
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Dec;21(14):3974-85
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Dec;21(14):3974-85
Thyroid hormone attenuates cardiac remodeling and improves hemodynamics early after acute myocardial infarction in rats.
Pantos C, Mourouzis I, Markakis K, Dimopoulos A, Xinaris C, Kokkinos AD, Panagiotou M, Cokkinos DV
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2007 Aug;32(2):333-9
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2007 Aug;32(2):333-9
UCP1-independent thermogenesis in white adipose tissue of cold-acclimated Ucp1-/- mice.
Ukropec J, Anunciado RP, Ravussin Y, Hulver MW, Kozak LP
The Journal of biological chemistry 2006 Oct 20;281(42):31894-908
The Journal of biological chemistry 2006 Oct 20;281(42):31894-908
Functional properties of human embryonic stem cell-derived cardiomyocytes: intracellular Ca2+ handling and the role of sarcoplasmic reticulum in the contraction.
Dolnikov K, Shilkrut M, Zeevi-Levin N, Gerecht-Nir S, Amit M, Danon A, Itskovitz-Eldor J, Binah O
Stem cells (Dayton, Ohio) 2006 Feb;24(2):236-45
Stem cells (Dayton, Ohio) 2006 Feb;24(2):236-45
Identification of a novel phosphorylation site in protein phosphatase inhibitor-1 as a negative regulator of cardiac function.
Rodriguez P, Mitton B, Waggoner JR, Kranias EG
The Journal of biological chemistry 2006 Dec 15;281(50):38599-608
The Journal of biological chemistry 2006 Dec 15;281(50):38599-608
Increased cardiac adenylyl cyclase expression is associated with increased survival after myocardial infarction.
Takahashi T, Tang T, Lai NC, Roth DM, Rebolledo B, Saito M, Lew WY, Clopton P, Hammond HK
Circulation 2006 Aug 1;114(5):388-96
Circulation 2006 Aug 1;114(5):388-96
Differential activation of stress-response signaling in load-induced cardiac hypertrophy and failure.
Rothermel BA, Berenji K, Tannous P, Kutschke W, Dey A, Nolan B, Yoo KD, Demetroulis E, Gimbel M, Cabuay B, Karimi M, Hill JA
Physiological genomics 2005 Sep 21;23(1):18-27
Physiological genomics 2005 Sep 21;23(1):18-27
Chronic ventricular myocyte-specific overexpression of angiotensin II type 2 receptor results in intrinsic myocyte contractile dysfunction.
Nakayama M, Yan X, Price RL, Borg TK, Ito K, Sanbe A, Robbins J, Lorell BH
American journal of physiology. Heart and circulatory physiology 2005 Jan;288(1):H317-27
American journal of physiology. Heart and circulatory physiology 2005 Jan;288(1):H317-27
Genetic manipulation of calcium-handling proteins in cardiac myocytes. I. Experimental studies.
Coutu P, Metzger JM
American journal of physiology. Heart and circulatory physiology 2005 Feb;288(2):H601-12
American journal of physiology. Heart and circulatory physiology 2005 Feb;288(2):H601-12
Protein kinase C and preconditioning: role of the sarcoplasmic reticulum.
Yamamura K, Steenbergen C, Murphy E
American journal of physiology. Heart and circulatory physiology 2005 Dec;289(6):H2484-90
American journal of physiology. Heart and circulatory physiology 2005 Dec;289(6):H2484-90
Impaired Ca2+ handling and contraction in cardiomyocytes from mice with a dominant negative thyroid hormone receptor alpha1.
Tavi P, Sjögren M, Lunde PK, Zhang SJ, Abbate F, Vennström B, Westerblad H
Journal of molecular and cellular cardiology 2005 Apr;38(4):655-63
Journal of molecular and cellular cardiology 2005 Apr;38(4):655-63
Phosphorylation-status of phospholamban and calsequestrin modifies their affinity towards commonly used antibodies.
Huke S, Periasamy M
Journal of molecular and cellular cardiology 2004 Sep;37(3):795-9
Journal of molecular and cellular cardiology 2004 Sep;37(3):795-9
Adenylyl cyclase type VI gene transfer reduces phospholamban expression in cardiac myocytes via activating transcription factor 3.
Gao MH, Tang T, Guo T, Sun SQ, Feramisco JR, Hammond HK
The Journal of biological chemistry 2004 Sep 10;279(37):38797-802
The Journal of biological chemistry 2004 Sep 10;279(37):38797-802
Doxycycline inducible expression of SERCA2a improves calcium handling and reverts cardiac dysfunction in pressure overload-induced cardiac hypertrophy.
Suarez J, Gloss B, Belke DD, Hu Y, Scott B, Dieterle T, Kim YK, Valencik ML, McDonald JA, Dillmann WH
American journal of physiology. Heart and circulatory physiology 2004 Nov;287(5):H2164-72
American journal of physiology. Heart and circulatory physiology 2004 Nov;287(5):H2164-72
The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm.
Radzyukevich TL, Moseley AE, Shelly DA, Redden GA, Behbehani MM, Lingrel JB, Paul RJ, Heiny JA
American journal of physiology. Cell physiology 2004 Nov;287(5):C1300-10
American journal of physiology. Cell physiology 2004 Nov;287(5):C1300-10
Alterations in myofilament function contribute to left ventricular dysfunction in pigs early after myocardial infarction.
van der Velden J, Merkus D, Klarenbeek BR, James AT, Boontje NM, Dekkers DH, Stienen GJ, Lamers JM, Duncker DJ
Circulation research 2004 Nov 26;95(11):e85-95
Circulation research 2004 Nov 26;95(11):e85-95
Improved expression and characterization of Ca2+-ATPase and phospholamban in High-Five cells.
Waggoner JR, Huffman J, Griffith BN, Jones LR, Mahaney JE
Protein expression and purification 2004 Mar;34(1):56-67
Protein expression and purification 2004 Mar;34(1):56-67
Accelerated onset of heart failure in mice during pressure overload with chronically decreased SERCA2 calcium pump activity.
Schultz Jel J, Glascock BJ, Witt SA, Nieman ML, Nattamai KJ, Liu LH, Lorenz JN, Shull GE, Kimball TR, Periasamy M
American journal of physiology. Heart and circulatory physiology 2004 Mar;286(3):H1146-53
American journal of physiology. Heart and circulatory physiology 2004 Mar;286(3):H1146-53
Frequency- and afterload-dependent cardiac modulation in vivo by troponin I with constitutively active protein kinase A phosphorylation sites.
Takimoto E, Soergel DG, Janssen PM, Stull LB, Kass DA, Murphy AM
Circulation research 2004 Mar 5;94(4):496-504
Circulation research 2004 Mar 5;94(4):496-504
Insights into cardioprotection obtained from study of cellular Ca2+ handling in myocardium of true hibernating mammals.
Yatani A, Kim SJ, Kudej RK, Wang Q, Depre C, Irie K, Kranias EG, Vatner SF, Vatner DE
American journal of physiology. Heart and circulatory physiology 2004 Jun;286(6):H2219-28
American journal of physiology. Heart and circulatory physiology 2004 Jun;286(6):H2219-28
Sarcoplasmic reticulum calcium defect in Ras-induced hypertrophic cardiomyopathy heart.
Zheng M, Dilly K, Dos Santos Cruz J, Li M, Gu Y, Ursitti JA, Chen J, Ross J Jr, Chien KR, Lederer JW, Wang Y
American journal of physiology. Heart and circulatory physiology 2004 Jan;286(1):H424-33
American journal of physiology. Heart and circulatory physiology 2004 Jan;286(1):H424-33
Mechanical unloading improves intracellular Ca2+ regulation in rats with doxorubicin-induced cardiomyopathy.
Takaseya T, Ishimatsu M, Tayama E, Nishi A, Akasu T, Aoyagi S
Journal of the American College of Cardiology 2004 Dec 7;44(11):2239-46
Journal of the American College of Cardiology 2004 Dec 7;44(11):2239-46
Autologous stem cell transplantation for myocardial repair.
Liu J, Hu Q, Wang Z, Xu C, Wang X, Gong G, Mansoor A, Lee J, Hou M, Zeng L, Zhang JR, Jerosch-Herold M, Guo T, Bache RJ, Zhang J
American journal of physiology. Heart and circulatory physiology 2004 Aug;287(2):H501-11
American journal of physiology. Heart and circulatory physiology 2004 Aug;287(2):H501-11
S100A1 gene transfer: a strategy to strengthen engineered cardiac grafts.
Remppis A, Pleger ST, Most P, Lindenkamp J, Ehlermann P, Schweda C, Löffler E, Weichenhan D, Zimmermann W, Eschenhagen T, Koch WJ, Katus HA
The journal of gene medicine 2004 Apr;6(4):387-94
The journal of gene medicine 2004 Apr;6(4):387-94
Ca2+/calmodulin-dependent protein kinase II phosphorylation regulates the cardiac ryanodine receptor.
Wehrens XH, Lehnart SE, Reiken SR, Marks AR
Circulation research 2004 Apr 2;94(6):e61-70
Circulation research 2004 Apr 2;94(6):e61-70
Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation.
Xu KY, Kuppusamy SP, Wang JQ, Li H, Cui H, Dawson TM, Huang PL, Burnett AL, Kuppusamy P, Becker LC
The Journal of biological chemistry 2003 Oct 24;278(43):41798-803
The Journal of biological chemistry 2003 Oct 24;278(43):41798-803
Mechanism of enhanced cardiac function in mice with hypertrophy induced by overexpressed Akt.
Kim YK, Kim SJ, Yatani A, Huang Y, Castelli G, Vatner DE, Liu J, Zhang Q, Diaz G, Zieba R, Thaisz J, Drusco A, Croce C, Sadoshima J, Condorelli G, Vatner SF
The Journal of biological chemistry 2003 Nov 28;278(48):47622-8
The Journal of biological chemistry 2003 Nov 28;278(48):47622-8
Targeted inhibition of Ca2+/calmodulin-dependent protein kinase II in cardiac longitudinal sarcoplasmic reticulum results in decreased phospholamban phosphorylation at threonine 17.
Ji Y, Li B, Reed TD, Lorenz JN, Kaetzel MA, Dedman JR
The Journal of biological chemistry 2003 Jul 4;278(27):25063-71
The Journal of biological chemistry 2003 Jul 4;278(27):25063-71
Effect of tachycardia on myocardial sarcoplasmic reticulum and Ca2+ dynamics: a mechanism for preconditioning?
Domenech RJ, Sánchez G, Donoso P, Parra V, Macho P
Journal of molecular and cellular cardiology 2003 Dec;35(12):1429-37
Journal of molecular and cellular cardiology 2003 Dec;35(12):1429-37
Effect of tachycardia on myocardial sarcoplasmic reticulum and Ca2+ dynamics: a mechanism for preconditioning?
Domenech RJ, Sánchez G, Donoso P, Parra V, Macho P
Journal of molecular and cellular cardiology 2003 Dec;35(12):1429-37
Journal of molecular and cellular cardiology 2003 Dec;35(12):1429-37
Gender differences in sarcoplasmic reticulum calcium loading after isoproterenol.
Chen J, Petranka J, Yamamura K, London RE, Steenbergen C, Murphy E
American journal of physiology. Heart and circulatory physiology 2003 Dec;285(6):H2657-62
American journal of physiology. Heart and circulatory physiology 2003 Dec;285(6):H2657-62
Regional alterations in protein expression in the dyssynchronous failing heart.
Spragg DD, Leclercq C, Loghmani M, Faris OP, Tunin RS, DiSilvestre D, McVeigh ER, Tomaselli GF, Kass DA
Circulation 2003 Aug 26;108(8):929-32
Circulation 2003 Aug 26;108(8):929-32
Altered dose response to beta-agonists in SERCA1a-expressing hearts ex vivo and in vivo.
Huke S, Prasad V, Nieman ML, Nattamai KJ, Grupp IL, Lorenz JN, Periasamy M
American journal of physiology. Heart and circulatory physiology 2002 Sep;283(3):H958-65
American journal of physiology. Heart and circulatory physiology 2002 Sep;283(3):H958-65
Defective intracellular Ca(2+) signaling contributes to cardiomyopathy in Type 1 diabetic rats.
Choi KM, Zhong Y, Hoit BD, Grupp IL, Hahn H, Dilly KW, Guatimosim S, Lederer WJ, Matlib MA
American journal of physiology. Heart and circulatory physiology 2002 Oct;283(4):H1398-408
American journal of physiology. Heart and circulatory physiology 2002 Oct;283(4):H1398-408
Abnormal Ca2+ release, but normal ryanodine receptors, in canine and human heart failure.
Jiang MT, Lokuta AJ, Farrell EF, Wolff MR, Haworth RA, Valdivia HH
Circulation research 2002 Nov 29;91(11):1015-22
Circulation research 2002 Nov 29;91(11):1015-22
Type 1 phosphatase, a negative regulator of cardiac function.
Carr AN, Schmidt AG, Suzuki Y, del Monte F, Sato Y, Lanner C, Breeden K, Jing SL, Allen PB, Greengard P, Yatani A, Hoit BD, Grupp IL, Hajjar RJ, DePaoli-Roach AA, Kranias EG
Molecular and cellular biology 2002 Jun;22(12):4124-35
Molecular and cellular biology 2002 Jun;22(12):4124-35
Type 1 phosphatase, a negative regulator of cardiac function.
Carr AN, Schmidt AG, Suzuki Y, del Monte F, Sato Y, Lanner C, Breeden K, Jing SL, Allen PB, Greengard P, Yatani A, Hoit BD, Grupp IL, Hajjar RJ, DePaoli-Roach AA, Kranias EG
Molecular and cellular biology 2002 Jun;22(12):4124-35
Molecular and cellular biology 2002 Jun;22(12):4124-35
Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart.
Toma C, Pittenger MF, Cahill KS, Byrne BJ, Kessler PD
Circulation 2002 Jan 1;105(1):93-8
Circulation 2002 Jan 1;105(1):93-8
Ca(2+) signaling in cardiac myocytes overexpressing the alpha(1) subunit of L-type Ca(2+) channel.
Song LS, Guia A, Muth JN, Rubio M, Wang SQ, Xiao RP, Josephson IR, Lakatta EG, Schwartz A, Cheng H
Circulation research 2002 Feb 8;90(2):174-81
Circulation research 2002 Feb 8;90(2):174-81
Ca(2+) signaling in cardiac myocytes overexpressing the alpha(1) subunit of L-type Ca(2+) channel.
Song LS, Guia A, Muth JN, Rubio M, Wang SQ, Xiao RP, Josephson IR, Lakatta EG, Schwartz A, Cheng H
Circulation research 2002 Feb 8;90(2):174-81
Circulation research 2002 Feb 8;90(2):174-81
Targeting phospholamban by gene transfer in human heart failure.
del Monte F, Harding SE, Dec GW, Gwathmey JK, Hajjar RJ
Circulation 2002 Feb 26;105(8):904-7
Circulation 2002 Feb 26;105(8):904-7
Targeting phospholamban by gene transfer in human heart failure.
del Monte F, Harding SE, Dec GW, Gwathmey JK, Hajjar RJ
Circulation 2002 Feb 26;105(8):904-7
Circulation 2002 Feb 26;105(8):904-7
Interaction between increased SERCA2a activity and beta -adrenoceptor stimulation in adult rabbit myocytes.
Chaudhri B, Del Monte F, Hajjar RJ, Harding SE
American journal of physiology. Heart and circulatory physiology 2002 Dec;283(6):H2450-7
American journal of physiology. Heart and circulatory physiology 2002 Dec;283(6):H2450-7
Cardiac remodeling and atrial fibrillation in transgenic mice overexpressing junctin.
Hong CS, Cho MC, Kwak YG, Song CH, Lee YH, Lim JS, Kwon YK, Chae SW, Kim DH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2002 Aug;16(10):1310-2
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2002 Aug;16(10):1310-2
Developmental changes of Ca(2+) handling in mouse ventricular cells from early embryo to adulthood.
Liu W, Yasui K, Opthof T, Ishiki R, Lee JK, Kamiya K, Yokota M, Kodama I
Life sciences 2002 Aug 2;71(11):1279-92
Life sciences 2002 Aug 2;71(11):1279-92
Developmental changes of Ca(2+) handling in mouse ventricular cells from early embryo to adulthood.
Liu W, Yasui K, Opthof T, Ishiki R, Lee JK, Kamiya K, Yokota M, Kodama I
Life sciences 2002 Aug 2;71(11):1279-92
Life sciences 2002 Aug 2;71(11):1279-92
Overexpression of the sarcoplasmic reticulum Ca(2+)-ATPase improves myocardial contractility in diabetic cardiomyopathy.
Trost SU, Belke DD, Bluhm WF, Meyer M, Swanson E, Dillmann WH
Diabetes 2002 Apr;51(4):1166-71
Diabetes 2002 Apr;51(4):1166-71
Overexpression of the sarcoplasmic reticulum Ca(2+)-ATPase improves myocardial contractility in diabetic cardiomyopathy.
Trost SU, Belke DD, Bluhm WF, Meyer M, Swanson E, Dillmann WH
Diabetes 2002 Apr;51(4):1166-71
Diabetes 2002 Apr;51(4):1166-71
Altered SR protein expression associated with contractile dysfunction in diabetic rat hearts.
Zhong Y, Ahmed S, Grupp IL, Matlib MA
American journal of physiology. Heart and circulatory physiology 2001 Sep;281(3):H1137-47
American journal of physiology. Heart and circulatory physiology 2001 Sep;281(3):H1137-47
mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope.
Kapiloff MS, Jackson N, Airhart N
Journal of cell science 2001 Sep;114(Pt 17):3167-76
Journal of cell science 2001 Sep;114(Pt 17):3167-76
mAKAP and the ryanodine receptor are part of a multi-component signaling complex on the cardiomyocyte nuclear envelope.
Kapiloff MS, Jackson N, Airhart N
Journal of cell science 2001 Sep;114(Pt 17):3167-76
Journal of cell science 2001 Sep;114(Pt 17):3167-76
S100A1: a regulator of myocardial contractility.
Most P, Bernotat J, Ehlermann P, Pleger ST, Reppel M, Börries M, Niroomand F, Pieske B, Janssen PM, Eschenhagen T, Karczewski P, Smith GL, Koch WJ, Katus HA, Remppis A
Proceedings of the National Academy of Sciences of the United States of America 2001 Nov 20;98(24):13889-94
Proceedings of the National Academy of Sciences of the United States of America 2001 Nov 20;98(24):13889-94
Impaired sarcoplasmic reticulum function leads to contractile dysfunction and cardiac hypertrophy.
Meyer M, Trost SU, Bluhm WF, Knot HJ, Swanson E, Dillmann WH
American journal of physiology. Heart and circulatory physiology 2001 May;280(5):H2046-52
American journal of physiology. Heart and circulatory physiology 2001 May;280(5):H2046-52
Interactions between phospholamban and beta-adrenergic drive may lead to cardiomyopathy and early mortality.
Dash R, Kadambi V, Schmidt AG, Tepe NM, Biniakiewicz D, Gerst MJ, Canning AM, Abraham WT, Hoit BD, Liggett SB, Lorenz JN, Dorn GW 2nd, Kranias EG
Circulation 2001 Feb 13;103(6):889-96
Circulation 2001 Feb 13;103(6):889-96
Interactions between phospholamban and beta-adrenergic drive may lead to cardiomyopathy and early mortality.
Dash R, Kadambi V, Schmidt AG, Tepe NM, Biniakiewicz D, Gerst MJ, Canning AM, Abraham WT, Hoit BD, Liggett SB, Lorenz JN, Dorn GW 2nd, Kranias EG
Circulation 2001 Feb 13;103(6):889-96
Circulation 2001 Feb 13;103(6):889-96
Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes.
Cavagna M, O'Donnell JM, Sumbilla C, Inesi G, Klein MG
The Journal of physiology 2000 Oct 1;528 Pt 1(Pt 1):53-63
The Journal of physiology 2000 Oct 1;528 Pt 1(Pt 1):53-63
Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers.
Froemming GR, Murray BE, Harmon S, Pette D, Ohlendieck K
Biochimica et biophysica acta 2000 Jun 1;1466(1-2):151-68
Biochimica et biophysica acta 2000 Jun 1;1466(1-2):151-68
Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers.
Froemming GR, Murray BE, Harmon S, Pette D, Ohlendieck K
Biochimica et biophysica acta 2000 Jun 1;1466(1-2):151-68
Biochimica et biophysica acta 2000 Jun 1;1466(1-2):151-68
Sprint training normalizes Ca(2+) transients and SR function in postinfarction rat myocytes.
Zhang LQ, Zhang XQ, Ng YC, Rothblum LI, Musch TI, Moore RL, Cheung JY
Journal of applied physiology (Bethesda, Md. : 1985) 2000 Jul;89(1):38-46
Journal of applied physiology (Bethesda, Md. : 1985) 2000 Jul;89(1):38-46
Sprint training normalizes Ca(2+) transients and SR function in postinfarction rat myocytes.
Zhang LQ, Zhang XQ, Ng YC, Rothblum LI, Musch TI, Moore RL, Cheung JY
Journal of applied physiology (Bethesda, Md. : 1985) 2000 Jul;89(1):38-46
Journal of applied physiology (Bethesda, Md. : 1985) 2000 Jul;89(1):38-46
Cardiac-specific overexpression of a superinhibitory pentameric phospholamban mutant enhances inhibition of cardiac function in vivo.
Zhai J, Schmidt AG, Hoit BD, Kimura Y, MacLennan DH, Kranias EG
The Journal of biological chemistry 2000 Apr 7;275(14):10538-44
The Journal of biological chemistry 2000 Apr 7;275(14):10538-44
Mechanisms of altered excitation-contraction coupling in canine tachycardia-induced heart failure, I: experimental studies.
O'Rourke B, Kass DA, Tomaselli GF, Kääb S, Tunin R, Marbán E
Circulation research 1999 Mar 19;84(5):562-70
Circulation research 1999 Mar 19;84(5):562-70
Mechanisms of altered excitation-contraction coupling in canine tachycardia-induced heart failure, I: experimental studies.
O'Rourke B, Kass DA, Tomaselli GF, Kääb S, Tunin R, Marbán E
Circulation research 1999 Mar 19;84(5):562-70
Circulation research 1999 Mar 19;84(5):562-70
Nitric oxide synthase in cardiac sarcoplasmic reticulum.
Xu KY, Huso DL, Dawson TM, Bredt DS, Becker LC
Proceedings of the National Academy of Sciences of the United States of America 1999 Jan 19;96(2):657-62
Proceedings of the National Academy of Sciences of the United States of America 1999 Jan 19;96(2):657-62
Nitric oxide synthase in cardiac sarcoplasmic reticulum.
Xu KY, Huso DL, Dawson TM, Bredt DS, Becker LC
Proceedings of the National Academy of Sciences of the United States of America 1999 Jan 19;96(2):657-62
Proceedings of the National Academy of Sciences of the United States of America 1999 Jan 19;96(2):657-62
In vivo phosphorylation of cardiac troponin I by protein kinase Cbeta2 decreases cardiomyocyte calcium responsiveness and contractility in transgenic mouse hearts.
Takeishi Y, Chu G, Kirkpatrick DM, Li Z, Wakasaki H, Kranias EG, King GL, Walsh RA
The Journal of clinical investigation 1998 Jul 1;102(1):72-8
The Journal of clinical investigation 1998 Jul 1;102(1):72-8
Regulation of Ca2+ handling by phosphorylation status in mouse fast- and slow-twitch skeletal muscle fibers.
Liu Y, Kranias EG, Schneider MF
The American journal of physiology 1997 Dec;273(6):C1915-24
The American journal of physiology 1997 Dec;273(6):C1915-24
Purified, reconstituted cardiac Ca2+-ATPase is regulated by phospholamban but not by direct phosphorylation with Ca2+/calmodulin-dependent protein kinase.
Reddy LG, Jones LR, Pace RC, Stokes DL
The Journal of biological chemistry 1996 Jun 21;271(25):14964-70
The Journal of biological chemistry 1996 Jun 21;271(25):14964-70
Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching.
Hu P, Yin C, Zhang KM, Wright LD, Nixon TE, Wechsler AS, Spratt JA, Briggs FN
The Journal of biological chemistry 1995 May 12;270(19):11619-22
The Journal of biological chemistry 1995 May 12;270(19):11619-22
Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching.
Hu P, Yin C, Zhang KM, Wright LD, Nixon TE, Wechsler AS, Spratt JA, Briggs FN
The Journal of biological chemistry 1995 May 12;270(19):11619-22
The Journal of biological chemistry 1995 May 12;270(19):11619-22
Ca(2+)-transporting ATPase, phospholamban, and calsequestrin levels in nonfailing and failing human myocardium.
Movsesian MA, Karimi M, Green K, Jones LR
Circulation 1994 Aug;90(2):653-7
Circulation 1994 Aug;90(2):653-7
Ca(2+)-transporting ATPase, phospholamban, and calsequestrin levels in nonfailing and failing human myocardium.
Movsesian MA, Karimi M, Green K, Jones LR
Circulation 1994 Aug;90(2):653-7
Circulation 1994 Aug;90(2):653-7
Residues 2-25 of phospholamban are insufficient to inhibit Ca2+ transport ATPase of cardiac sarcoplasmic reticulum.
Jones LR, Field LJ
The Journal of biological chemistry 1993 Jun 5;268(16):11486-8
The Journal of biological chemistry 1993 Jun 5;268(16):11486-8
The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle.
Jorgensen AO, Shen AC, Arnold W, McPherson PS, Campbell KP
The Journal of cell biology 1993 Feb;120(4):969-80
The Journal of cell biology 1993 Feb;120(4):969-80
The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle.
Jorgensen AO, Shen AC, Arnold W, McPherson PS, Campbell KP
The Journal of cell biology 1993 Feb;120(4):969-80
The Journal of cell biology 1993 Feb;120(4):969-80
Comparative studies of cardiac and skeletal sarcoplasmic reticulum ATPases. Effect of a phospholamban antibody on enzyme activation by Ca2+.
Cantilina T, Sagara Y, Inesi G, Jones LR
The Journal of biological chemistry 1993 Aug 15;268(23):17018-25
The Journal of biological chemistry 1993 Aug 15;268(23):17018-25
Phospholamban expressed in slow-twitch and chronically stimulated fast-twitch muscles minimally affects calcium affinity of sarcoplasmic reticulum Ca(2+)-ATPase.
Briggs FN, Lee KF, Wechsler AW, Jones LR
The Journal of biological chemistry 1992 Dec 25;267(36):26056-61
The Journal of biological chemistry 1992 Dec 25;267(36):26056-61
Phospholamban mediates the beta-adrenergic-enhanced Ca2+ uptake in mammalian ventricular myocytes.
Sham JS, Jones LR, Morad M
The American journal of physiology 1991 Oct;261(4 Pt 2):H1344-9
The American journal of physiology 1991 Oct;261(4 Pt 2):H1344-9
Phospholamban mediates the beta-adrenergic-enhanced Ca2+ uptake in mammalian ventricular myocytes.
Sham JS, Jones LR, Morad M
The American journal of physiology 1991 Oct;261(4 Pt 2):H1344-9
The American journal of physiology 1991 Oct;261(4 Pt 2):H1344-9
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- Western blot analysis of Phospholamban was performed by loading 25 µg of human heart (lane 1) and C2C12 (lane 2) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Phospholamban monoclonal antibody (Product # MA3-922) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~25 kDa.
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- Immunofluorescent analysis of Phospholamban (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospholamban monoclonal antibody (Product # MA3-922) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
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- Figure 4 Calcium Handling Properties of hiPSC-derived cardiomyocytes under High Glucose Condition and Empagliflozin. ( A ) Representative tracings of whole-cell calcium transients in hiPSC-derived cardiomyocytes; (B) Amplitudes of whole-cell calcium transients; (C) Maximal upstroke velocity of whole-cell calcium transients; and (D) Maximal decay velocity of whole-cell calcium transients. Abbreviations: NG: normal glucose; HG: high glucose; HG + E: high glucose and empagliflozin; and HG, HG + E: high glucose for 7 days followed with empagliflozin. (E) The expression of ATP2A2 , RYR2 and NCX1 was evaluated by quantitative PCR analysis using the expression of TNNT2 as an internal control. Abbreviations: NG: normal glucose; HG: high glucose; HG + E: high glucose and empagliflozin; and HG, HG + E: high glucose for 7 days followed with empagliflozin. (F) Protein level of phospho-phospholamban and total phospholamban was evaluated by Western blot analysis, the protein level of beta-actin was used as internal reference. * p < 0.05 and ** p < 0.01. For the full-length image of gels and blots shown in this figure, please refer to Supplementary Figs S4 and S5 .
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- Figure 2--figure supplement 2. Analysis of RNA and protein expression levels of the major cardiac Ca 2+ -handling proteins in PLN/DWORF Tg mice. ( A ) RNA levels of the indicated genes as quantified by qRT-PCR in 16-week-old heart tissue. Atp2a2 , SERCA2a; Ryr2 , ryanodine receptor 2; Cacna1c, alpha1C-subunit of the L-type Ca 2+ channel; Casq2 , calsequestrin 2. Data are normalized to 18S and presented as expression level relative to WT, mean +-SD for n = 10 mice per genotype. ( B ) Representative immunoblots of cardiac homogenates from mice with the indicated genotypes. PS16, phospho-serine 16 on PLN; PT17, phospho-threonine 17 on PLN; tPLN, total phospholamban; RyR2, ryanodine receptor 2; LTCC, L-type Ca 2+ channel (alpha1C-subunit); Casq2, calsequestrin 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. ( C ) Western blots for n = 9-10 mice of each genotype were quantified using ImageJ and data are normalized to GAPDH and expressed as mean +-SD relative to WT. ( D ) Quantification of total phospholamban and its phosphorylation status as assessed by western blot ( B ) and expressed as relative to WT. Western blots were quantified with ImageJ software. Phosphorylation blots (PS16 and PT17) were normalized to total PLN (tPLN). Total PLN was normalized to GAPDH. Data are expressed as mean +-SD for n = 9-10 mice per genotype. Statistical comparisons between groups were evaluated by Student's t-test. p-value *p
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- Figure 2--figure supplement 3. DWORF binding to SERCA2a displaces PLN in a dose-dependent manner and enhances SERCA activity. ( A ) Immunoprecipitations of Myc-SERCA2a were performed on lysates of HEK293 cells co-transfected with equal amounts of HA-PLN and Myc-SERCA2a (1 ug) and increasing amounts of HA-DWORF. Western blots on input samples (bottom) and bound immunoprecipitated fractions (top) reveal that DWORF binding to SERCA2a competitively displaces PLN. ( B, C ) Ca 2+ -dependent Ca 2+ -uptake assays were performed using homogenates from HEK293 cells co-transfected with equal amounts of SERCA2a and PLN (1 ug) and increasing amounts of DWORF. Co-transfection of cells with PLN (dark grey line) results in a rightward shift of the Ca 2+ -affinity curve as compared to control cells (SERCA2a alone, black) ( B ). Co-transfection with increasing levels of DWORF relieves the inhibitory effect of PLN on SERCA2a in a dose-dependent manner as evidenced by a leftward shift of the affinity curve back toward control values. Cells expressing SERCA2a and DWORF in the absence of PLN do not exhibit enhanced SERCA activity (purple), indicating that DWORF exerts its' stimulatory effect on SERCA through the displacement of PLN. ( C ) Mean K Ca values from n = 4 separate experiments are represented as bar graphs (+-SD). p-value *p
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- Figure 6--figure supplement 1. Expression and post-translational modifications of Ca 2+ -handling proteins. ( A ) Immunoblots of cardiac homogenates from mice with the indicated genotypes. ( B ) Immunoblots were quantified using ImageJ and normalized to GAPDH. LTCC, L-type Ca 2+ channel (alpha1C-subunit); Casq2, calsequestrin 2; RyR2, ryanodine receptor 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. ( C ) Quantification of the phosphorylation status and oligomerization of phospholamban as assessed by western blot (panel A ) and expressed as relative to WT. Western blots were quantified with ImageJ software. Phosphorylation blots (PS16 and PT17) were normalized to total PLN (tPLN). Total PLN was normalized to GAPDH. tPLN, total phospholamban; PS16, phospho-serine 16 on PLN; PT17, phospho-threonine 17 on PLN. Data are expressed as mean +-SD for n = 4-6 mice per genotype. ( D ) RNA levels of the indicated genes as quantified by qRT-PCR in 8-week-old heart tissue. Atp2a2 , SERCA2a; Cacna1c, alpha1C-subunit of the L-type Ca 2+ channel; Casq2 , calsequestrin 2; Pln , phosholamban; Ryr2 , ryanodine receptor 2. Data are normalized to 18S values and presented as expression level relative to WT, mean +-SD for n = 4-5 mice per genotype.
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- Figure 5 Comparison of protein expression in LV sample from control and cardiomyopathic patients. A: Top; representative SERCA2 and respective GAPDH stainings obtained in normal and cardiomyopathic LV samples. Bottom; Mean+-SEM, *** P
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- Fig 6 Immunoblotting. Immunoblot analysis of key Ca 2+ handling proteins and phosphorylation was performed on tissue from left ventricles. SERCA2A abundance (A). PLB abundance (B) and phosphorylation on SER16 (C) and THR17 (D). NCX abundance (E). RyR abundance (F) with phosphorylation on SER2808 (G) and SER2814 (H). CaMKII abundance (I) and CaMKII phosphorylation on THR286 (J). PP1 (K) and PP2A (L) abundance. n heart = 6 for all analysis. *p
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- Fig 5 Densitometric analysis of Western blots. (A) SERCA2a; (B) phospholamban (PLB), (C) PLB to SERCA2a ratio, in hearts from male and female sham and gonadectomized rats. (SM: sham male; SF: sham female; GM: gonadectomized male; GF: gonadectomized female). The values are expressed as the means +-S.E.M (n = 6 per group). *P < 0.05 vs. females of the same group; + P < 0.05 vs. sham animals of the same sex (two-way ANOVA and Fischer test).
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- Figure 5 Expressions of PLB (A), pPLB-ser16 (B), PKA (C) and PP-1 (D)normalyzed by beta-actin. PKA: protein kinase A; PLB:dephosphorylated phospholamban; pPLB-ser16: phosphorylatedphospholamban in serin-16; PP-1: phosphatase-1. Control (n = 6) andobese (n = 6). Data are expressed as mean +- standard-deviation.Student's t-test * p
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- Fig 8 TRPC4beta attenuates hypertrophic growth in cardiomyocytes after GPCR stimulation. A, Hypertrophic growth of cardiomyocytes was measured as an increase of the cell surface after angiotensin II/phenylephrine (Ang II/PE) or vehicle (CON) treatment for 24 h. Red: alpha-actinin; Blue: DAPI. * P
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- Fig 4 Alterations of sarcoplasmic reticulum Ca 2+ -ATPase (Serca) 2a and its regulator phospholamban (PLN) during AF. Total PLN ( a ) and PLN phosphorylated by CaMKII (Thr17; b ) or PKA (Ser16; c ) was downregulated in AF animals. d Phosphorylation levels relative to total PLN. e Serca2a protein analysis revealed a trend towards reduced protein expression associated with AF. Mean +- SEM optical density data normalized to GAPDH obtained from n = 5 Western blots per group are displayed. * P < 0.05; ** P < 0.01.
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- 2 Protein expression in embryonic chicken cardiac myocytes Embryonic chicken cardiac myocytes were infected with Ad.Sr1 (expressing SERCA1 ) or Ad.Sr2a (expressing SERCA2a ), and cell lysates were probed with various monoclonal antibodies. A , right, antibody to the c-myc tag found only in exogenous SERCA1 and SERCA2a; middle, antibody to SERCA1; left, antibody to endogenous and exogenous SERCA2a. Lane headings: C, control; Sr1, Ad.Sr1 infected; Sr2a, Ad.Sr2a infected. B , antibody to phospholamban. C , antibody to calsequestrin. For B and C , each lane was run in duplicate.
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- Fig. 4 SGO1 interaction with HCN4. a SGO1-WT and SGO1-K23E transduction did not change HCN1, HCN2, HCN3, or HCN4 mRNA expression in NRVMs. n = 7 biologically independent preparations, each exposed in parallel to each condition. Data are expressed as mean +- SEM, normalized to respective control sample result for each preparation. b, c Transduction of SGO1-WT or SGO1-K23E did not affect HCN2 protein expression. n = 5 biologically independent preparations, each exposed in parallel to each condition. Data are expressed as mean +- SEM, normalized to respective control sample result for each preparation. d, e Transduction of SGO1-WT or SGO1-K23E did not change HCN4 protein expression. n = 5 biologically independent preparations, each exposed in parallel to each condition. Data are expressed as mean +- SEM, normalized to respective control sample result for each preparation. f Co-IP of native SGO1 with HCN4 in non-transduced NRVMs. NRVM lysates were incubated with HCN4 antibody or rabbit IgG (isotype antibody control); SGO1 antibody was used to detect SGO1 bands. Similar results were obtained in n = 5 biologically independent experiments. g A reverse co-IP with phospholamban (PLN) antibody as the isotypic antibody control. NRVM lysates were loaded as the input control in f and g . IP: immunoprecipitation. WB: Western blot. Similar results were obtained in n = 5 biologically independent experiments. h Confocal images from NRVM slides showing colocalization between native HCN4 and SG
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- Figure 5 Effects of hyperaldosteronism and/or hypoestrogenism on calcium-handling regulatory proteins in wild type ( WT ) or aldostereone synthase overexpressing ( AS ) mouse hearts . (A) shows representative immunoblots of ryanodine receptor (RyR2), sarcoplasmic reticulum Ca 2+ - ATP ase ( SERCA 2a), phospholamban ( PLN ) and its phosphorylation (Thr17 or Ser16), and Na + /Ca 2+ -exchanger ( NCX ). (B-G) illustrate their respective quantification. The values expressed as mean +- SEM and normalized to GAPDH expression protein level ( n = 5-10 in each group).* P < 0.05; ** P < 0.01, and ### P < 0.001.
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- Figure 4 Effect of pressure overload on RV cardiomyocytes and calcium handling. ( A ) cardiomyocytes passive tension; ( B ) cardiomyocytes active tension; ( C ) myofilaments calcium sensitivity; ( D ) Hill -coefficient; ( E ) the rate of tension redevelopment (Ktr); Protein quantification of: ( F ) sarcoplasmic/endoplasmic reticulum Ca 2+ ATPase 2a (SERCA2a); ( G ) ratio of phospholamban phosphorylated (p-PLB) to total (PLB); ( H ) ratio of SERCA2a to phospholamban; ( I ) sodium-calcium exchanger (NCX); ( I ) Junctophilin; ( L ) representative western blot lanes (for representative purposes the image was cropped and edited) representative membrane protein loading. n = 5 for each group. Values are mean +- SEM, One-way ANOVA. Ba/Deb vs Sh: alpha p < 0.05; alphaalpha p < 0.01; alphaalphaalpha p < 0.001; Deb vs Ba: chi p < 0.05; chichichi p < 0.001.
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- Fig. 6 Western blot of EC-coupling proteins in human-induced pluripotent stem cell cardiomyocytes (iPSC-CM). ( a ) Representative original Western blots after treatment with empagliflozin (EMPA) or vehicle control (control) for 2 or 8 weeks from 4 differentiation experiments (Diff.) from 2 healthy donors. GAPDH was used as loading control. ( b ) Mean protein expression levels (normalized to the respective control group at 2 or 8 weeks) in iPSC-CM ( n = 4 differentiations, matched groups are displayed with matched individual symbols) and effects of 2 and 8 weeks treatment with empagliflozin (EMPA) on ryanodine-receptor type 2 (RyR2), ( c ) sodium-calcium exchanger (NCX), ( d ) sarcoplasmic reticulum Ca 2+ ATPase (SERCA), ( e ) phosphorylated Ca 2+ -/calmodulin-dependent protein kinase II (pCaMKII), ( f ) Ca 2+ -/calmodulin-dependent protein kinase II (CaMKII), and ( g ) phospholamban (PLB). Groups were statistically analyzed using two-way ANOVA with Sidak's test for multiple comparisons
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- Figure 4 Cardiac myocyte Ca 2+ -handling proteins in mouse mammary tumor virus-polyomavirus middle T antigen (MMTV-PyMT)- and MMTV-PyMT+ mice--phospholamban ( A ); SERCA2a (sarcoplasmic or endoplasmic reticulum calcium 2 ATPase) ( B ); phosphorilated phospholamban (p-phospholamban) ( C ); sodium/calcium exchanger (NCX) ( D ); and SERCA2a/phospholambam ratio ( E ). Western blot bands are shown for each protein. In the MMTV-PyMT+ mice, phospholamban protein expression was increased ( P =0.011) and SERCA2a protein expression was not different between the MMTV-PyMT+ and MMTV-PyMT- groups ( P =0.165). SERCA2a/phospholamban ratio was reduced in the MMTV-PyMT+ mice ( P =0.007). p-Phospholamban and NCX protein expression were decreased ( P =0.038 and P =0.017, respectively) compared with MMTV-PyMT- mice. Mann-Whitney test. Values are expressed as medians and interquartile ranges (IQRs). Animals per group: 7 MMTV-PyMT- and 7 MMTV-PyMT+.
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- Fig. 2 Repeated subcutaneous injection of PLN ASO in mice with PLN R14 Delta/Delta prevents cardiac dysfunction and improves survival. a Experimental design of the PLN R14 Delta/Delta intervention study, consisting of ASO#27 treatment with a 4-week loading phase with weekly dosing of 100 mg/kg and a 20-week maintenance phase with dosing of 50 mg/kg once every 4 weeks. Data points for functional and biochemical assessments were obtained at treatment week 4 (T4) which was the primary endpoint, 15 (T15), 19 (T19), and 23 (T23) at the end of the study. b SYBR green qPCR results of cardiac Pln mRNA expression ( n = 3 for wild-type [WT] and n = 4 for PBS and PLN-ASO). c Semi-quantified western blot analysis of PLN protein expression using LV protein lysates. Intensities were normalized to total protein loading control and the PBS treated mice ( n = 3 per group). d Immunofluorescence of WT, PBS treated and PLN-ASO treated PLN R14 Delta/Delta mice after 4 weeks treatment. Sections were co-stained for cardiac Troponin T (green), PLN (red), and DAPI (blue). Note the lower presence of visible PLN aggregations (white arrows) and the higher structural integrity of the PLN-ASO treated hearts vs. PBS control. Stainings were performed in two animals per group. e Representative MRI images after 4 weeks of treatment. f Quantification of LVEF (left ventricular ejection fraction), LVESV (left ventricular end-systolic volume), and LVEDV (left ventricular end-diastolic volume) ( n = 12 for PBS T4,
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- Fig. 3 Repeated subcutaneous injection of PLN ASO in Cspr3/Mlp -/- mice reverses signs and symptoms of heart failure. a Experimental design of the PLN-ASO Cspr3/Mlp -/- intervention study. Repeated studies were run with either PLN-ASO #27 (100 mg/kg on Days 0, 7, 14, and 21) or PLN-ASO #26_C (100 mg/kg on day 0, 50 mg/kg on days 7 and 14) (Supplementary Data 1 ). Echocardiography was performed at baseline (i.e., before treatment initiation), and at end of study (i.e., after 28 days of treatment), followed by termination of animals. Hemodynamic assessment was done between day 24 and 28. b Representative Western blot of LV protein lysates stained for PLN protein to detect the monomer and pentamer. Protein loading was normalized to GAPDH and total PLN protein were semi-quantified by calculating intensities relative to PBS treated control (PBS n = 10, PLN-ASO #26_C n = 10). c Individual echocardiography assessment and quantification of left ventricular ejection fraction (LVEF), left ventricular end-systolic volume (LVESV), and left ventricular end-diastolic volume (LVEDV) 28 days after treatment relative to baseline measurements. Cspr3/Mlp -/- mice with LVEF
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- Figure 2 Histological and molecular analysis of PLN-R14del mice hearts. ( A ) Principal component analysis plot of RNA-Seq analysis of left ventricles of WT, PLN-R14 Delta/+ and PLN-R14 Delta/Delta mice of 3 or 8 weeks of age (n = 4 per group). 95% confidence ellipses of clusters are marked in grey. Information on genes contributing to variance in principle component 1 (PC1, x-axis) and principle component 2 (PC2, y-axis) is shown in Supplementary Fig. 1B . ( B ) qPCR measurements of left ventricular mRNA levels of Nppa (ANP), Nppb (BNP), and the ratio of Myh7 (beta-MHC) to Myh6 (alpha-MHC) of 8-week-old WT, PLN-R14 Delta/+ and PLN-R14 Delta/Delta mice (n = 4, 5, and 5, respectively). ( C ) Representative images of left ventricular sections stained with Masson's trichrome (scale bar = 70 mum) with ( D ) quantification of myocardial fibrosis in WT, PLN-R14 Delta/+ and PLN-R14 Delta/Delta mice hearts (n = 4, 5, and 5, respectively). ( E,F ) qPCR measurements of left ventricular mRNA levels of cardiac remodelling genes Col1a1 , Col1a2 , Col3a1 , Lgals3 (Gal-3), Timp1 , and Mmp2 of 8-week-old WT, PLN-R14 Delta/+ and PLN-R14 Delta/Delta mice (n = 4, 5, and 5, respectively). ( G ) Representative images of left ventricular sections of 8-week-old WT, PLN-R14 Delta/+ and PLN-R14 Delta/Delta mice stained with an anti-PLN antibody (red), wheat-germ agglutinin (WGA) (green), and DAPI (blue) (scale bar = 35 mum). ( H ) Western blot analysis of monomeric PLN proteins in RIPA-soluble and RI
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- Figure 3 Cardiac functional, histological and molecular analysis of 12- to 20-month-old PLN-R14 Delta/+ mice. Echocardiographic analysis of left ventricular end-diastolic diameter ( A ), end-systolic diameter ( B ), fractional shortening ( C ), and global longitudinal strain ( D ) of 12-, 18- and 20-month-old WT and PLN-R14 Delta/+ mice (n = 9, 10, 6, 10, 5 and 9, respectively). ( E ) qPCR measurements of left ventricular mRNA levels of Nppa (ANP), Nppb (BNP), and the ratio of Myh7 (beta-MHC) to Myh6 (alpha-MHC) of 20-month-old WT and PLN-R14 Delta/+ mice (n = 6 and 10, respectively). ( F ) Representative images of left ventricular sections of 20-month-old WT and PLN-R14 Delta/+ mice stained with an anti-PLN antibody (red), wheat-germ agglutinin (WGA) (green), and DAPI (blue) (scale bar = 35 mum) or ( G ) stained with Masson's trichrome (scale bar = 70 mum) with ( H ) quantification of myocardial fibrosis (n = 6 and 9, respectively). ( I,J ) qPCR measurements of left ventricular mRNA levels of cardiac remodelling genes Col1a1 , Col1a2 , Col3a1 , Lgals3 (Gal-3), Timp1 , and Mmp2 of 20-month-old WT and PLN-R14 Delta/+ mice (n = 6 and 10, respectively). Gene expression values are corrected for Rplp0 (36B4) gene expression, and shown as fold change compared to age-matched WT. Myocardial fibrosis is presented as fold change compared to age-matched WT. Data are presented as mean +- S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001 compared to age-matched WT (Mann-Whitney test).
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- Figure 2 IL-6 neutralization prevents atrial inflammation and the alterations in Ca 2+ handling proteins in SP rats. (A, B) Representative images of atrial MPO staining (A) and quantification (B) in the 4 indicated groups. Five visual fields are taken in each sample, and the number of immune cells from these fields is quantified with ImagePro 6.0 software and is averaged to make a statistical analysis. n = 6-7/group. Scale bar: 50 um. (C, D) Representative images of atrial CD68 staining (C) and quantification (D) . n = 6-7/group. Scale bar: 50 um. (E-I) Original Western blot (E) and quantification of the expression of RyR2, p-RyR2 (Ser2808), p-RyR2 (Ser2814) (F) , the expression of SERCA and NCX (G) , SERCA activity (H) , the expression of PLB, p-PLB (Ser16), and p-PLB (Thr17) (I) , in atrial tissue of 4 indicated groups. n =6-8/group. * P < 0.05, *** P < 0.001 vs . Sham; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . IgG, determined by Student t-test or one-way ANOVA with Bonferroni's post-hoc test. (F-H) .
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- Figure 8 Exogenous IL-6 administration increases the alterations in Ca 2+ handling proteins and AF induction in vivo . (A-E) Original Western blot (A) and quantification the expression of RyR2, p-RyR2 (Ser2808), p-RyR2 (Ser2814) (B) , the expression of SERCA and NCX (C) , SERCA activity (D) , the expression of PLB, p-PLB (Ser16), and p-PLB (Thr17) (E) , in atrial tissue of 2 indicated groups. n = 6/group. (F, G) Statistical results of AF duration (F) and probability (G) in the 2 indicated groups. n = 8/group. * P < 0.05, ** P < 0.01, *** P < 0.001 vs . Control determined by Student t-test.
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- Fig. 4. Phospholamban is down-regulated at day 3. Sarcoplasmic reticulum Ca 2&plus pump (SERCA; A ) and phospholamban (PLB; B ) expression in mice at baseline, 12 h after lipopolysaccharide (LPS) administration, and at day 3. Upper panels show illustrative Western blots of SERCA, phospholamban, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in left ventricle extracts. Lower panels shown average data, normalized to GAPDH (loading control), and shown as a ratio of baseline. N = 6 hearts each. Phospholamban was visualized in the pentameric (25 kilodalton &lsqbkd&rsqb) and tetrameric forms (20 kd). Average data shown are for the predominant 20-kd isoform, and similar results were obtained for the 25-kd isoform. Data are shown as mean +- SD. BL = baseline. &ast P < 0.05 versus baseline.
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- Figure 2. PP1s differentially regulate substrate phosphorylation in neonatal cardiac myocytes Western blot analysis for the phosphorylation of PLB at serine 16 and threonine 17, total PLB, and PP1s from neonatal rat cardiac myocytes infected with adenoviruses expressing either beta-gal (Con) or individual PP1 isoform. p-MLC2 was performed using ProQ-Diamond staining method. Quantification was based on individual western blot. N = 4 for each group. * P < 0.05 vs. Con 5 min.
