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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP53331PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53331PU-N, RRID:AB_11148963
- Product name
- anti Phospholamban (Ser16)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide selected from the Ser16 region of Human Phospholamban
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-PLB-pSer16 Cat.-No AP53331PU-N in A375 cell line lysates (36ug/lane). PLB (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using PLB-Ser16 Antibody Cat.-No AP53331PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLB-S16 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A375 cells using PLB-Ser16 Antibody Cat.-No AP53331PU-N (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
