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Learn209-406-B50
antibody from Rockland Immunochemicals, Inc.
Targeting: IL33
C9orf26, DKFZp586H0523, DVS27, IL1F11, NF-HEV
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 209-406-B50 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#209-406-B50, RRID:AB_10705200
- Product name
- Anti-Human IL-33 (RABBIT) Antibody Biotin Conjugated - 209-406-B50
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recom-binant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
- Validation comment
- Western Blot
