AF4859

antibody from Novus Biologicals

Targeting: DDX58 DKFZp434J1111, FLJ13599, RIG-1, RIG-I, RIG1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4859

Provider

Novus Biologicals

Product name

Goat Polyclonal RIG-I Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human RIG-I in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human RIG-I by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RIG-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4859) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RIG-I at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.