Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C344741 - Provider product page
- Provider
- LSBio
- Product name
- DDX58 / RIG-1 / RIG-I Antibody (aa201-713, clone Alme-1, Biotin) LS-C344741
- Antibody type
- Monoclonal
- Description
- Protein G purified from tissue culture supernatant
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- Alme-1
- Storage
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of human RIG-I in HeLa cells by using RIG-I, mAb (Alme-1) . Method: Cell extracts from HeLa cells either unstimulated (lane 1) or stimulated for 6h (lane 2), 16h (lane 3) or 24 h (lane 4) with Interferon gamma were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the RIG-I , mAb (Alme-1) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemical staining of endogenous human RIG-I in different human tissues (paraffin sections) using anti-RIG-I, mAb (Alme-1) . .Different human normal tissues (A: Nasopharynx; B: Colon; C: Endometrium) or cancer tissues (D: Breast; E: Skin; F: Ovarian) were stained with anti-RIG-I, mAb (Alme-1) by standard immunohistochemistry. This image was taken for the unconjugated form of this product. Other forms have not been tested.