Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-4121-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-RIG-I Antibody
- Antibody type
- Monoclonal
- Description
- RIG-I (retinoic-acid-inducible gene I), also known as DDX58 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 58) is a 925-residue cytoplasmic viral RNA receptor, critically involved in the activation of the innate immune response to RNA virus infection. It is a member of the RIG-I-like receptor (RLR) family and is an essential intracellular sensor for several 5-triphosphorylated RNA viruses. RIG-I elicits its antiviral interferon (IFN) responses by recognizing viral double-stranded RNAs (dsRNAs). Structurally it comprises a helicase domain, a C-terminal domain, and N-terminal CARDs (caspase activation recruitment domains) involved in activating MAVS (mitochondrial antiviral signaling protein). Upon binding of 5?-triphosphorylated RNA, RIG-I undergoes conformational changes and post-translational modifications that allow multimerization and interaction with the mitochondrial antiviral signaling protein (MAVS).
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant human
RIG-I protein (amino acids 1-220) w
as used as the immunogen for this a
ntibody.- Isotype
- IgG
- Antibody clone number
- ABM4H29
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of RIG1/DDx58. Anti-RIG1/DDx58 antibody (Clone: ABM4H29) was tested at 2 µg/ml on human kidney lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RIG1/DDx58 in human Colon Tissue using RIG1/DDx58 antibody (Clone: ABM4H29) at 15 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RIG1/DDx58 in human Spleen Tissue using RIG1/DDx58 antibody (Clone: ABM4H29) at 15 µg/ml.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 µg/10^6 cells of Anti-RIGI antibody (ABM4H29). Green represent isotype control and red represent Anti-RIG I antibody (10-4121 Abeomics). Goat anti-mouse PE conjugate was used as secondary.
- Protocol
- Protocol