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antibody from Invitrogen Antibodies
Targeting: DDX58
DKFZp434J1111, FLJ13599, RIG-1, RIG-I, RIG1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Other assay [5]
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- Product number
- PA5-20276 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RIG-I Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- A suggested positive control is C2C12 cell lysate.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references The Causal Role of Mitochondrial Dynamics in Regulating Innate Immunity in Diabetes.
Therapeutically Active RIG-I Agonist Induces Immunogenic Tumor Cell Killing in Breast Cancers.
Chang YH, Lin HY, Shen FC, Su YJ, Chuang JH, Lin TK, Liou CW, Lin CY, Weng SW, Wang PW
Frontiers in endocrinology 2020;11:445
Frontiers in endocrinology 2020;11:445
Therapeutically Active RIG-I Agonist Induces Immunogenic Tumor Cell Killing in Breast Cancers.
Elion DL, Jacobson ME, Hicks DJ, Rahman B, Sanchez V, Gonzales-Ericsson PI, Fedorova O, Pyle AM, Wilson JT, Cook RS
Cancer research 2018 Nov 1;78(21):6183-6195
Cancer research 2018 Nov 1;78(21):6183-6195
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of RIG-1 in MCF7 cells with RIG-I Polyclonal Antibody (Product # PA5-20276) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Role of MFN1/MFN2 overexpression on innate immune response in cybrid B4 under nutrient excess. Cybrid B4 cells were overexpressed with GFP, GFP-MFN1, or GFP-MFN2 for 18 h, followed by a 6-h exposure with or without 0.25 mM FA. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. Quantification value of beta-actin was used for normalization. * p < 0.05 compared to vector control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Role of MFN1/MFN2 knockdown on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. Quantification value of beta-actin was used for normalization. * p < 0.05 compared to siRNA control in the absence of FA or between indicated groups. + p < 0.05 comparison between indicated groups. CON, control; FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Role of DRP1/FIS1 knockdown on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to siRNA control in the absence of FA. + p < 0.05 or between indicated groups. CON, control; FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Role of DRP1/FIS1 overexpression on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to vector control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Effect of mdivi-1 on innate immune response in cybrid B4 under nutrient excess. Cybrid B4 cells were treated with/without FA for 6 h, followed by 10 muM mdivi-1 for another 24 h. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to mock control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.
