200-301-E95

antibody from Rockland Immunochemicals, Inc.

Targeting: KCNMB2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

200-301-E95

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-301-E95, RRID:AB_2611124

Product name

Anti-BK channel BETA2 (MOUSE) Monoclonal Antibody

Antibody type

Monoclonal

Antigen

Recombinant Protein

Description

Anti-BK channel BETA2 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with BK channel BETA2 from human, mouse, and rat based on 100% homology with the immunizing sequence. Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin. Cross-reactivity with BK channel BETA2 from other sources has not been determined. Ion Channels research.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

1mg/mL by UV absorbance at 280 nm

Storage

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Handling

Dry Ice

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Mouse anti-BK channel BETA2 antibody. Lane 1: COS-1 cells transiently transfected with KBeta2 lysate. Load: 10ug. Priamary antibody: BK channel BETA2 at 1:1000 overnight at 4°C. Secondary antibody: Goat anti-mouse IgG HRP at 1:40,000 for 45 min at RT. Blocked: 5% Blotto overnight at 4°C. Predicated/observed size: 27.1 kDa, 30 kDa for BK channel BETA2.

Validation comment

Western Blot