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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 600-401-A73 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-A73, RRID:AB_2258425
- Product name
- Anti-Fibroblast Activation Protein (FAP) (RABBIT) Antibody - 600-401-A73
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000. Personal communication, S.Kim, NCI, Bethesda, MD.
- Validation comment
- Western Blot
