Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- ASC-004-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCN3A (NaV1.3) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat newborn brain membranes: - 1. Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:200). 2. Anti-SCN3A (NaV1.3) Antibody , preincubated with SCN3A/Nav1.3 Blocking Peptide (#BLP-SC004).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NaV1.3 in rat DRG primary cells - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglia (DRG) primary culture. A. DRG cells were stained using Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:200) followed by goat Anti-rabbit-AlexaFluor-555 secondary Antibody . B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NaV1.3 in rat embryo DRG - Immunohistochemical staining of rat embryo dorsal root ganglion (DRG) frozen sections using Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:100). NaV1.3 is expressed in DRG embryonic cells (arrows). Calibration bar = 50 mu m.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NaV1.3 in rat embryo DRG - Immunohistochemical staining of rat embryo dorsal root ganglion (DRG) frozen sections using Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:100). NaV1.3 is expressed in DRG embryonic cells (arrows). Calibration bar = 50 mu m.