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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
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- Product number
- AF3428 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Lyp/PTPN22 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Lyp in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references PTPN22.6, a dominant negative isoform of PTPN22 and potential biomarker of rheumatoid arthritis.
Autoimmune-associated PTPN22 R620W variation reduces phosphorylation of lymphoid phosphatase on an inhibitory tyrosine residue.
Chang HH, Tai TS, Lu B, Iannaccone C, Cernadas M, Weinblatt M, Shadick N, Miaw SC, Ho IC
PloS one 2012;7(3):e33067
PloS one 2012;7(3):e33067
Autoimmune-associated PTPN22 R620W variation reduces phosphorylation of lymphoid phosphatase on an inhibitory tyrosine residue.
Fiorillo E, Orrú V, Stanford SM, Liu Y, Salek M, Rapini N, Schenone AD, Saccucci P, Delogu LG, Angelini F, Manca Bitti ML, Schmedt C, Chan AC, Acuto O, Bottini N
The Journal of biological chemistry 2010 Aug 20;285(34):26506-18
The Journal of biological chemistry 2010 Aug 20;285(34):26506-18
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Lyp by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell lines. PVDF membrane was probed with 0.3 µg/mL of Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Lyp at approximately 108 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Lyp by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lyp at approximately 116-118 kDa (as indicated) using 3 µg/mL of Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
