AF3428

antibody from R&D Systems

Targeting: PTPN22 Lyp, Lyp1, Lyp2, PTPN8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3428

Provider

R&D Systems

Product name

Human Lyp Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Lyp in Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9Y2R2


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

PTPN22.6, a dominant negative isoform of PTPN22 and potential biomarker of rheumatoid arthritis.

Chang HH, Tai TS, Lu B, Iannaccone C, Cernadas M, Weinblatt M, Shadick N, Miaw SC, Ho IC
PloS one 2012;7(3):e33067

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Autoimmune-associated PTPN22 R620W variation reduces phosphorylation of lymphoid phosphatase on an inhibitory tyrosine residue.

Fiorillo E, Orrú V, Stanford SM, Liu Y, Salek M, Rapini N, Schenone AD, Saccucci P, Delogu LG, Angelini F, Manca Bitti ML, Schmedt C, Chan AC, Acuto O, Bottini N
The Journal of biological chemistry 2010 Aug 20;285(34):26506-18

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Lyp by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell lines. PVDF membrane was probed with 0.3 µg/mL of Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Lyp at approximately 108 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Lyp by Simple Western^TM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lyp at approximately 116-118 kDa (as indicated) using 3 µg/mL of Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Lyp in Human PBMCs. Lyp was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.