NB200-403
antibody from Novus Biologicals
Targeting: PAPPA
ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A, PAPPA1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- NB200-403 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-403, RRID:AB_10001102
- Product name
- Mouse Monoclonal Pappalysin-1/PAPP-A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. PAPPA (3C8)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PAPP A Antibody (3C8) [NB200-403] - Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blotting. Lane 1: 18A10 (NB100-73059) Lane 2: 7A6 (NB100-73060) Lane 3: 3C8 (NB200-402) Lane 4: 5H9 (proMBP-specific) (NB110-8423) Lane 5: 11E4 (proMBP-specific) (NB120-14597) Lane 6: 7A6 (NB100-73060) Lane 7: 10A5 (NB200-402) Lane 8: 3C8 (NB200-403) Lane 9: 10E1 (NB120-8346) Lanes 1-5: after SDS-PAGE in reduction conditions. Lanes 6-9: Non-reducing conditions. Heterotetrameric complex was detected by anti-PAPP-A MAbs
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PAPP A Antibody (3C8) [NB200-403] - Figure A - SDS-PAGE analysis in reducing conditions of human recombinant dPAPP-A and tissue (purified from atherosclerotic plaques) dPAPP-A. Lane 1: human htPAPP-A Lane 2: recombinant dPAPP-A Lane 3: tissue dPAPP-A Gel staining: Coomassie brilliant blue R-250 Comments: proMBP subunit released from htPAPP-A upon reduction is not stained by Coomassie brilliant blue because of high degree of glycosylation. Figure B - Western blotting analysis of PAPP-A purified from atherosclerotic tissue. For immunostaining mixture of anti-PAPP-A MAbs 18A10 (Cat # NB100-73059), 7A6(Cat # NB100-73060) and 3C8 (Cat # NB200-403) Lanes are noted as in Figure A.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PAPP A Antibody (3C8) [NB200-403] - Calibration curves for PAPP-A sanwich immunoassay. (A) 4G11 3C8 Capture Mab: 4G11 (biotinylated) Detection MAbs: 3C8 (labeled with stable Eu3+ -chelate) Antigen: htPAPP-A