NB100-73059

antibody from Novus Biologicals

Targeting: PAPPA ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A, PAPPA1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-73059

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-73059, RRID:AB_1109623

Product name

Mouse Monoclonal Pappalysin-1/PAPP-A Antibody

Antibody type

Monoclonal

Description

Protein A purified. Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and pro-MBP subunits).

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.2 mg

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Pappalysin-1/PAPP-A Antibody (18A10) [NB100-73059] - A - SDS-PAGE analysis in reducing conditions of human recombinant dPAPP-A and tissue (purified from atherosclerotic plaques) dPAPP-A. Lane 1: human htPAPP-A Lane 2: recombinant dPAPP-A Lane 3: tissue dPAPP-A Gel staining: Coomassie brilliant blue R-250 Comments: proMBP subunit released from htPAPP-A upon reduction is not stained by Coomassie brilliant blue because of high degree of glycosylation. B - Western blotting analysis of PAPP-A purified from atherosclerotic tissue. For immunostaining mixture of anti-PAPP-A MAbs 18A10 (Cat # NB100-73059), 7A6(Cat # NB100-73060) and 3C8 (Cat # NB200-403) Lanes are noted as in Fig.4A