ABIN2508699

antibody from antibodies-online

Targeting: LEP OB, OBS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2508699

Provider

antibodies-online

Product name

anti-Leptin (LEP) antibody (Biotin)

Antibody type

Polyclonal

Antigen

Other

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLeptin. Anti-Human Leptin specific antibody was purified by affinity chromatography and then biotinylated.

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Vial size

50 μg

Storage

-20°C

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat skeletal muscle lysate (lanes 3 and 7) and human SH-SY5Y neuroblastoma cell lysate (lanes 4 and 8): 1- 4. Anti-Na+/Ca2+ Exchanger 2 (NCX-2) antibody (ABIN2511041), (1:200). 5- 8. Anti-Na+/Ca2+ Exchanger 2 (NCX-2) antibody, preincubated with the control peptide antigen. Expression of NCX2 in rat cerebellum Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Na+/Ca2+ Exchanger 2 (NCX-2) antibody (ABIN2511041), (1:100). A. NCX2 staining (red) is expressed in the molecular layer (M) and Purkinje cells (arrows). B. DAPI counterstain (blue) displays the layout of cerebellar layers. C. Merge of A and B demonstrates restriction of NCX2 expression to the molecular layer and Purkinje cells.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Image(s): Immunofluorescence