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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Immunohistochemistry [1]
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- Product number
- A00479-4 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Leptin Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Leptin detection. Tested with WB, IHC-P, ELISA(Cap) in Mouse;Rat.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Trigonelline Inhibits Inflammation and Protects β Cells to Prevent Fetal Growth Restriction during Pregnancy in a Mouse Model of Diabetes.
Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid.
Testis dysfunction by isoproterenol is mediated by upregulating endothelin receptor A, leptin and protein kinase Cvarepsilon and is attenuated by an endothelin receptor antagonist CPU0213.
Stress-induced cardiac insufficiency relating to abnormal leptin and FKBP12.6 is ameliorated by CPU0213, an endothelin receptor antagonist, which is not affected by the CYP3A suppressing effect of erythromycin.
Zhou JY, Du XH, Zhang Z, Qian GS
Pharmacology 2017;100(5-6):209-217
Pharmacology 2017;100(5-6):209-217
Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid.
Chen L, Wang T, Chen G, Wang N, Gui L, Dai F, Fang Z, Zhang Q, Lu Y
Endocrine 2017 Mar;55(3):773-785
Endocrine 2017 Mar;55(3):773-785
Testis dysfunction by isoproterenol is mediated by upregulating endothelin receptor A, leptin and protein kinase Cvarepsilon and is attenuated by an endothelin receptor antagonist CPU0213.
Cheng YS, Dai DZ, Dai Y
Reproductive toxicology (Elmsford, N.Y.) 2010 Jul;29(4):421-6
Reproductive toxicology (Elmsford, N.Y.) 2010 Jul;29(4):421-6
Stress-induced cardiac insufficiency relating to abnormal leptin and FKBP12.6 is ameliorated by CPU0213, an endothelin receptor antagonist, which is not affected by the CYP3A suppressing effect of erythromycin.
Cheng YS, Dai DZ, Dai Y
The Journal of pharmacy and pharmacology 2009 May;61(5):569-76
The Journal of pharmacy and pharmacology 2009 May;61(5):569-76
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Leptin using anti-Leptin antibody (A00479-4).Leptin was detected in paraffin-embedded section of mouse lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Leptin Antibody (A00479-4) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
