PB9099

antibody from Boster Biological Technology

Targeting: COL4A1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PB9099

Provider

Boster Biological Technology

Product name

Anti-Collagen IV/COL4A1 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for COLLAGEN IV/COL4A1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IF. Reactive species: Human. COLLAGEN IV/COL4A1 information: Molecular Weight: 138564 MW; Subcellular Localization: Secreted, extracellular space, extracellular matrix ; Tissue Specificity: Highly expressed in placenta.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Collagen ??using anti-Collagen ??antibody (PB9099). Collagen ??was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen ??Antibody (PB9099) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image