MA1-70057

antibody from Invitrogen Antibodies

Targeting: CFH ARMD4, ARMS1, FHL1, HF, HF1, HF2, HUS

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-70057

Provider

Invitrogen Antibodies

Product name

Complement Factor H Monoclonal Antibody (OX-24)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-70057 detects Complement Factor H in human samples. MA1-70057 has been successfully used in flow cytometry, ELISA, immunoprecipitation and Western blot procedures. The MA1-70057 immunogen is human complement Factor H. Store at 4ºC. For long term storage, aliquot and freeze unused portion at -20ºC in volumes appropriat for single usage. Avoid freeze/thaw cycles.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OX-24

Vial size

250 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Complement Genetic Variants and FH Desialylation in S. pneumoniae-Haemolytic Uraemic Syndrome.

Gómez Delgado I, Corvillo F, Nozal P, Arjona E, Madrid Á, Melgosa M, Bravo J, Szilágyi Á, Csuka D, Veszeli N, Prohászka Z, Sánchez-Corral P
Frontiers in immunology 2021;12:641656

---

Gain-of-function factor H-related 5 protein impairs glomerular complement regulation resulting in kidney damage.

Malik TH, Gitterman DP, Lavin DP, Lomax-Browne HJ, Hiemeyer EC, Moran LB, Boroviak K, Cook HT, Gilmore AC, Mandwie M, Ahmad A, Alexander IE, Logan GJ, Marchbank KJ, Bradley A, Pickering MC
Proceedings of the National Academy of Sciences of the United States of America 2021 Mar 30;118(13)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4. HDM-FH ameliorates glomerular C3 in hFH-FHR5mut mice. ( A ) Representative glomerular images and ( B ) staining scores for C3b/iC3b/C3c, C3d, FHR5mut, FH, IgG, and properdin in male hFH-FHR5mut mice treated with either AAV-GFP ( n = 5, black dots) or AAV-HDM-FH ( n = 7). Bars denote the median, and whiskers denote the interquartile range. * P = 0.0013, derived from Mann-Whitney U test. Plasma hFH/HDM-FH ( C ), C3 ( D ), and FHR5mut ( E ) levels after administration of either AAV-GFP or AAV-HDM-FH to hFH-FHR5mut male mice. Bars denote the mean, and whiskers denote the SD. P values are derived from two-way ANOVA with Bonferroni multiple comparisons test.