AF4999

antibody from R&D Systems

Targeting: CFH ARMD4, ARMS1, FHL1, HF, HF1, HF2, HUS

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4999

Provider

R&D Systems

Product name

Mouse Complement Factor H Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Complement Factor H in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Complement Factor H is observed.

Reactivity

Mouse

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

NP_034018


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse Complement Factor H by Simple Western^TM. Simple Western lane view shows mouse plasma, loaded at 0.2 mg/mL. A specific band was detected for Complement Factor H at approximately 166 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse Complement Factor H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4999) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse Complement Factor H by Western Blot. Western blot shows lysates of Hepa 1-6 mouse hepatoma cell line, mouse liver tissue, and mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4999) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor H at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.