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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21302 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Complement Factor H Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Molt-4, human plasma. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Complement factor H using 30 µg of MOLT4 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a Complement factor H polyclonal antibody (Product # PA5-21302) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Complement Factor H was performed by separating 30 µg of human tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Complement Factor H Polyclonal Antibody (Product # PA5-21302) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Factor H (Product # PA5-21302) antibody at 1:200 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
