18167-1-AP

antibody from Proteintech Group

Targeting: PRKAA2 AMPK, AMPKa2, PRKAA

Provider product page for 18167-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [50]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 18167-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#18167-1-AP, RRID:AB_10695046
Product name: AMPK Alpha 2 antibody
Antibody type: Polyclonal
Description: KD/KO validated AMPK Alpha 2 antibody (Cat. #18167-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PRKAA2 protein structure - 18167-1-AP shown in red.

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Trinh-Minh T, Györfi AH, Tomcik M, Tran-Manh C, Zhou X, Dickel N, Tümerdem BS, Kreuter A, Burmann SN, Borchert SV, Hussain RI, Hallén J, Klingelhöfer J, Kunz M, Distler JHW
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Polyphyllin II (PPII) Enhances the Sensitivity of Multidrug-resistant A549/DDP Cells to Cisplatin by Modulating Mitochondrial Energy Metabolism.

Peng L, Yang R, Wang Z, Jian H, Tan X, Li J, He Z, Huang R, Zeng P, Gao W
In vivo (Athens, Greece) 2024 Jan-Feb;38(1):213-225

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis.

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Nature communications 2024 Apr 6;15(1):2974

Integrative analysis of multi-omics data for discovery of ferroptosis-related gene signature predicting immune activity in neuroblastoma.

Hu J, Song F, Kang W, Xia F, Song Z, Wang Y, Li J, Zhao Q
Frontiers in pharmacology 2023;14:1162563

Fatty acid synthesis and oxidation regulate human endoderm differentiation by mediating SMAD3 nuclear localization via acetylation.

Yi Y, Lan X, Li Y, Yan C, Lv J, Zhang T, Jiang W
Developmental cell 2023 Sep 25;58(18):1670-1687.e4

DJ1 Ameliorates AD-like Pathology in the Hippocampus of APP/PS1 Mice.

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Resolvin D1 reprograms energy metabolism to promote microglia to phagocytize neutrophils after ischemic stroke.

Li L, Cheng SQ, Sun YQ, Yu JB, Huang XX, Dong YF, Ji J, Zhang XY, Hu G, Sun XL
Cell reports 2023 Jun 27;42(6):112617

Quantification and Proteomic Characterization of β-Hydroxybutyrylation Modification in the Hearts of AMPKα2 Knockout Mice.

Ding WJ, Li XH, Tang CM, Yang XC, Sun Y, Song YP, Ling MY, Yan R, Gao HQ, Zhang WH, Yu N, Feng JC, Zhang Z, Xing YQ
Molecular & cellular proteomics : MCP 2023 Feb;22(2):100494

Mailuoning oral liquid attenuates convalescent cerebral ischemia by inhibiting AMPK/mTOR-associated apoptosis and promoting CREB/BDNF-mediated neuroprotection.

Liu X, Fan L, Li J, Bai Z, Wang Y, Liu Y, Jiang H, Tao A, Li X, Zhang H, Tan N
Journal of ethnopharmacology 2023 Dec 5;317:116731

RGCC-mediated PLK1 activity drives breast cancer lung metastasis by phosphorylating AMPKα2 to activate oxidative phosphorylation and fatty acid oxidation.

Cheng S, Wan X, Yang L, Qin Y, Chen S, Liu Y, Sun Y, Qiu Y, Huang L, Qin Q, Cui X, Wu M, Liu M
Journal of experimental & clinical cancer research : CR 2023 Dec 15;42(1):342

Anti-ferroptotic PRKAA2 serves as a potential diagnostic and prognostic marker for hepatoblastoma.

Xie Y, Cui Z, Fang S, Zhu G, Zhen N, Zhu J, Mao S, Sun F, Pan Q, Ma J
Journal of gastrointestinal oncology 2023 Aug 31;14(4):1788-1805

Dibutyl phthalate induces epithelial-mesenchymal transition of renal tubular epithelial cells via the Ang II/AMPKα2/Cx43 signaling pathway.

Zhiwen X, Yongqing Z, Wenlan S, Shan H, Bangmin H, Juntao J, Yingjian Z, Yifeng J
Toxicology 2023 Aug 1;494:153584

Astaxanthin promotes mitochondrial biogenesis and antioxidant capacity in chronic high-intensity interval training.

Wang Y, Chen X, Baker JS, Davison GW, Xu S, Zhou Y, Bao X
European journal of nutrition 2023 Apr;62(3):1453-1466

Targeting USP9X-AMPK Axis in ARID1A-Deficient Hepatocellular Carcinoma.

Zhang FK, Ni QZ, Wang K, Cao HJ, Guan DX, Zhang EB, Ma N, Wang YK, Zheng QW, Xu S, Zhu B, Chen TW, Xia J, Qiu XS, Ding XF, Jiang H, Qiu L, Wang X, Chen W, Cheng SQ, Xie D, Li JJ
Cellular and molecular gastroenterology and hepatology 2022;14(1):101-127

Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function.

He L, Zhou X, Wu Z, Feng Y, Liu D, Li T, Yin Y
Animal nutrition (Zhongguo xu mu shou yi xue hui) 2022 Sep;10:137-147

Remote cyclic compression ameliorates myocardial infarction injury in rats via AMPK-dependent pathway.

Xu S, Xia X, Liu Y, Chen F, Gu R, Bian X, Xu X, Jia C, Lu S, Gu Y, Bai H, Zhang H
Microvascular research 2022 May;141:104313

Sestrin2 overexpression attenuates osteoarthritis pain via induction of AMPK/PGC-1α-mediated mitochondrial biogenesis and suppression of neuroinflammation.

Sun J, Song FH, Wu JY, Zhang LQ, Li DY, Gao SJ, Liu DQ, Zhou YQ, Mei W
Brain, behavior, and immunity 2022 May;102:53-70

Transcriptomics combined with metabolomics analysis of the mechanism of agmatine in the treatment of septic liver injury.

Huang L, Gan L, Pan J, Zhong L, Wang Q, Luo S, Tian J, Liang H
Annals of translational medicine 2022 May;10(10):578

Metformin Relieves Bortezomib-Induced Neuropathic Pain by Regulating AMPKa2-Mediated Autophagy in the Spinal Dorsal Horn.

Liu M, Zhao YT, Lv YY, Xu T, Li D, Xiong YC, Xin WJ, Lin SY
Neurochemical research 2022 Jul;47(7):1878-1887

Bupivacaine reduces GlyT1 expression by potentiating the p-AMPKα/BDNF signalling pathway in spinal astrocytes of rats.

Lu K, Zhao L, Zhang Y, Yang F, Zhang H, Wang J, Li B, Ji G, Yu J, Ma H
Scientific reports 2022 Jan 26;12(1):1378

Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion.

Wang JQ, Li LL, Hu A, Deng G, Wei J, Li YF, Liu YB, Lu XY, Qiu ZP, Shi XJ, Zhao X, Luo J, Song BL
Nature 2022 Aug;608(7922):413-420

Phosphorylated STYK1 restrains the inhibitory role of EGFR in autophagy initiation and EGFR-TKIs sensitivity.

Zhou C, Dong X, Wang M, Qian X, Hu M, Liang K, Liang Y, Zhang R, Huang Y, Lyu H, Xiao S, Tang Y, Ali DW, Michalak M, Chen XZ, Tang J
Cell insight 2022 Aug;1(4):100045

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Cao Z, Pan J, Sui X, Fang C, Li N, Huang X, Qu X, Han D
Evidence-based complementary and alternative medicine : eCAM 2021;2021:5590623

UBE2O promotes hepatocellular carcinoma cell proliferation and invasion by regulating the AMPKα2/mTOR pathway.

Shi Z, Liu R, Lu Q, Zeng Z, Liu Y, Zhao J, Liu X, Li L, Huang H, Yao Y, Huang D, Xu Q
International journal of medical sciences 2021;18(16):3749-3758

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Zhou C, Liang Y, Zhou L, Yan Y, Liu N, Zhang R, Huang Y, Wang M, Tang Y, Ali DW, Wang Y, Michalak M, Chen XZ, Tang J
Autophagy 2021 Oct;17(10):3175-3195

SIRT3 affects mitochondrial metabolic reprogramming via the AMPK-PGC-1α axis in the development of benign prostatic hyperplasia.

Li Y, Wang Q, Li J, Shi B, Liu Y, Wang P
The Prostate 2021 Nov;81(15):1135-1148

Stanniocalcin-1 Protected Astrocytes from Hypoxic Damage Through the AMPK Pathway.

Sun B, He S, Liu B, Xu G, Guoji E, Feng L, Xu L, Chen D, Zhao W, Chen J, Gao Y, Zhang E
Neurochemical research 2021 Nov;46(11):2948-2957

Naringin induces skeletal muscle fiber type transformation via AMPK/PGC-1α signaling pathway in mice and C2C12 myotubes.

Xue Y, Huang Z, Chen X, Jia G, Zhao H, Liu G
Nutrition research (New York, N.Y.) 2021 Aug;92:99-108

Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.

Huang L, Yang X, Deng L, Ying D, Lu A, Zhang L, Yu A, Duan B
ACS applied materials & interfaces 2021 Apr 14;13(14):16106-16117

Metformin Mitigates Cartilage Degradation by Activating AMPK/SIRT1-Mediated Autophagy in a Mouse Osteoarthritis Model.

Wang C, Yao Z, Zhang Y, Yang Y, Liu J, Shi Y, Zhang C
Frontiers in pharmacology 2020;11:1114

Si-Miao-Yong-An Decoction attenuates isoprenaline-induced myocardial fibrosis in AMPK-driven Akt/mTOR and TGF-β/SMAD3 pathways.

Zhao Y, Sun D, Chen Y, Zhan K, Meng Q, Zhang X, Zhu L, Yao X
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Yang L, Cao H, Sun D, Lin L, Zheng WP, Shen ZY, Song HL
Stem cells and development 2020 Jul 1;29(13):835-852

UBE2O promotes the proliferation, EMT and stemness properties of breast cancer cells through the UBE2O/AMPKα2/mTORC1-MYC positive feedback loop.

Liu X, Ma F, Liu C, Zhu K, Li W, Xu Y, Li G, Niu Z, Liu J, Chen D, Li Z, Fu Y, Qian C
Cell death & disease 2020 Jan 6;11(1):10

Whether AICAR in Pregnancy or Lactation Prevents Hypertension Programmed by High Saturated Fat Diet: A Pilot Study.

Tsai WL, Hsu CN, Tain YL
Nutrients 2020 Feb 11;12(2)

Activation of AMPK suppresses S1P-induced airway smooth muscle cells proliferation and its potential mechanisms.

Pan Y, Liu L, Zhang Q, Shi W, Feng W, Wang J, Wang Q, Li S, Li M
Molecular immunology 2020 Dec;128:106-115

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Fu Y, Sun Y, Wang M, Hou Y, Huang W, Zhou D, Wang Z, Yang S, Tang W, Zhen J, Li Y, Wang X, Liu M, Zhang Y, Wang B, Liu G, Yu X, Sun J, Zhang C, Yi F
Cell metabolism 2020 Dec 1;32(6):1052-1062.e8

Dual-specificity phosphatase 29 is induced during neurogenic skeletal muscle atrophy and attenuates glucocorticoid receptor activity in muscle cell culture.

Cooper LM, West RC, Hayes CS, Waddell DS
American journal of physiology. Cell physiology 2020 Aug 1;319(2):C441-C454

Hepatitis C virus nonstructural protein 5A perturbs lipid metabolism by modulating AMPK/SREBP-1c signaling.

Meng Z, Liu Q, Sun F, Qiao L
Lipids in health and disease 2019 Nov 4;18(1):191

Activation of AMPK inhibits TGF-β1-induced airway smooth muscle cells proliferation and its potential mechanisms.

Pan Y, Liu L, Li S, Wang K, Ke R, Shi W, Wang J, Yan X, Zhang Q, Wang Q, Chai L, Xie X, Li M
Scientific reports 2018 Feb 26;8(1):3624

Apelin‑13 ameliorates metabolic and cardiovascular disorders in a rat model of type 2 diabetes with a high‑fat diet.

Li M, Fang H, Hu J
Molecular medicine reports 2018 Dec;18(6):5784-5790

Antagonizing peroxisome proliferator-activated receptor γ facilitates M1-to-M2 shift of microglia by enhancing autophagy via the LKB1-AMPK signaling pathway.

Ji J, Xue TF, Guo XD, Yang J, Guo RB, Wang J, Huang JY, Zhao XJ, Sun XL
Aging cell 2018 Aug;17(4):e12774

Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis.

Zhao Z, Zhang L, Guo XD, Cao LL, Xue TF, Zhao XJ, Yang DD, Yang J, Ji J, Huang JY, Sun XL
Frontiers in molecular neuroscience 2017;10:293

PD149163 induces hypothermia to protect against brain injury in acute cerebral ischemic rats.

Xue TF, Ding X, Ji J, Yan H, Huang JY, Guo XD, Yang J, Sun XL
Journal of pharmacological sciences 2017 Nov;135(3):105-113

AMPK/α-Ketoglutarate Axis Regulates Intestinal Water and Ion Homeostasis in Young Pigs.

He L, Huang N, Li H, Tian J, Zhou X, Li T, Yao K, Wu G, Yin Y
Journal of agricultural and food chemistry 2017 Mar 22;65(11):2287-2298

AMP-activated protein kinase-mediated expression of heat shock protein beta 1 enhanced insulin sensitivity in the skeletal muscle.

Yuan H, Wang T, Niu Y, Liu X, Fu L
FEBS letters 2017 Jan;591(1):97-108

AMPK deficiency in chondrocytes accelerated the progression of instability-induced and ageing-associated osteoarthritis in adult mice.

Zhou S, Lu W, Chen L, Ge Q, Chen D, Xu Z, Shi D, Dai J, Li J, Ju H, Cao Y, Qin J, Chen S, Teng H, Jiang Q
Scientific reports 2017 Feb 22;7:43245

Extracellular pH regulates autophagy via the AMPK-ULK1 pathway in rat cardiomyocytes.

Zhao L, Cui L, Jiang X, Zhang J, Zhu M, Jia J, Zhang Q, Zhang J, Zhang D, Huang Y
FEBS letters 2016 Sep;590(18):3202-12

Activation of AMPK α2 inhibits airway smooth muscle cells proliferation.

Liu L, Pan Y, Song Y, Su X, Ke R, Yang L, Gao L, Li M
European journal of pharmacology 2016 Nov 15;791:235-243

Knockdown of AMPKα2 Promotes Pulmonary Arterial Smooth Muscle Cells Proliferation via mTOR/Skp2/p27(Kip1) Signaling Pathway.

Ke R, Liu L, Zhu Y, Li S, Xie X, Li F, Song Y, Yang L, Gao L, Li M
International journal of molecular sciences 2016 May 31;17(6)

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Dong K, Wu M, Liu X, Huang Y, Zhang D, Wang Y, Yan LJ, Shi D
Free radical biology & medicine 2016 Dec;101:334-347

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18167-1-AP(PRKAA2 antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using PRKAA2 antibody 18167-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line