Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB100-238 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-238, RRID:AB_10001819
- Product name
- Rabbit Polyclonal AMPK alpha 2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The evidence of metabolic-improving effect of metformin in Ay/a mice with genetically-induced melanocortin obesity and the contribution of hypothalamic mechanisms to this effect.
High CO2 levels cause skeletal muscle atrophy via AMP-activated kinase (AMPK), FoxO3a protein, and muscle-specific Ring finger protein 1 (MuRF1).
Alpha1-AMP-activated protein kinase regulates hypoxia-induced Na,K-ATPase endocytosis via direct phosphorylation of protein kinase C zeta.
Metabolic activation of AMP kinase in vascular smooth muscle.
Derkach K, Zakharova I, Zorina I, Bakhtyukov A, Romanova I, Bayunova L, Shpakov A
PloS one 2019;14(3):e0213779
PloS one 2019;14(3):e0213779
High CO2 levels cause skeletal muscle atrophy via AMP-activated kinase (AMPK), FoxO3a protein, and muscle-specific Ring finger protein 1 (MuRF1).
Jaitovich A, Angulo M, Lecuona E, Dada LA, Welch LC, Cheng Y, Gusarova G, Ceco E, Liu C, Shigemura M, Barreiro E, Patterson C, Nader GA, Sznajder JI
The Journal of biological chemistry 2015 Apr 3;290(14):9183-94
The Journal of biological chemistry 2015 Apr 3;290(14):9183-94
Alpha1-AMP-activated protein kinase regulates hypoxia-induced Na,K-ATPase endocytosis via direct phosphorylation of protein kinase C zeta.
Gusarova GA, Dada LA, Kelly AM, Brodie C, Witters LA, Chandel NS, Sznajder JI
Molecular and cellular biology 2009 Jul;29(13):3455-64
Molecular and cellular biology 2009 Jul;29(13):3455-64
Metabolic activation of AMP kinase in vascular smooth muscle.
Rubin LJ, Magliola L, Feng X, Jones AW, Hale CC
Journal of applied physiology (Bethesda, Md. : 1985) 2005 Jan;98(1):296-306
Journal of applied physiology (Bethesda, Md. : 1985) 2005 Jan;98(1):296-306
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: AMPK alpha 2 Antibody [NB100-238] - Detection of Human and Mouse AMPK alpha 2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-AMPK alpha 2 antibody NB100-238 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.