MA5-33216

antibody from Invitrogen Antibodies

Targeting: PRKAA2 AMPK, AMPKa2, PRKAA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-33216

Provider

Invitrogen Antibodies

Product name

Phospho-AMPK alpha-2 (Thr172) Recombinant Rabbit Monoclonal Antibody (4F4)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

4F4

Vial size

100 µL

Concentration

3 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Phospho-AMPK alpha-2 (Thr172) using a Phospho-AMPK alpha-2 (Thr172) Monoclonal antibody (Product # MA5-33216) at a concentration of 1.95 µg/mL. Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate(treated with Calyculin A or EGF). All lanes: Phospho-PRKAA2 antibody at 1.95µg/ml. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 62 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Phospho-AMPK alpha-2 (Thr172) using a Phospho-AMPK alpha-2 (Thr172) Monoclonal antibody (Product # MA5-33216) at a concentration of 1.95 µg/mL. Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate(treated with Calyculin A or EGF). All lanes: Phospho-PRKAA2 antibody at 1.95µg/ml. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 62 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-AMPK alpha-2 (Thr172) in A549 cells using a Phospho-AMPK alpha-2 (Thr172) monoclonal antibody (Product # MA5-33216) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Cells were counter-stained with DAPI.