PA5-114550

antibody from Invitrogen Antibodies

Targeting: PRKAA2 AMPK, AMPKa2, PRKAA

Western blot

Antibody data

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Validation data

Product number: PA5-114550 - Provider product page
Provider: Invitrogen Antibodies
Product name: Phospho-AMPK alpha-1,2 (Thr172) Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Antibody detects endogenous levels of AMPK alpha 1 when phosphorylated at Thr183, or AMPK alpha 2 when phosphorylated at Thr172.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 1 mg/mL
Storage: -20°C

PRKAA2 protein structure - PA5-114550 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in C2C12 cells. Samples were treated with PFA, permeabilized in 0.1% saponin prior to blocking in 10% serum (45 min at 37°C), and incubated with polyclonal antibody (Product # PA5-114550) at a dilution of 1:400 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in 293 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114550) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in C2C12 cells. Samples were treated with PFA, permeabilized in 0.1% saponin prior to blocking in 10% serum (45 min at 37°C), and incubated with polyclonal antibody (Product # PA5-114550) at a dilution of 1:400 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in C2C12 cells. Samples were treated with PFA, permeabilized in 0.1% saponin prior to blocking in 10% serum (45 min at 37°C), and incubated with polyclonal antibody (Product # PA5-114550) at a dilution of 1:400 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in human colon. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114550) at a dilution of 1:100. Secondary staining was applied with HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in rat skin tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114550) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in mouse testis tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114550) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in rat spleen tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114550) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in human gastric cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114550) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in mouse kidney tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114550) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in human colorectal cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114550) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in rat lung tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114550) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in human mammary cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114550) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.