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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- PA5-21494 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMPK alpha-2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3. Predicted reactivity: Mouse (98%), Rat (98%), Pig (99%), Chicken (96%), Sheep (98%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.96 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- AMPK alpha-2 Polyclonal Antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample: 1,000 µg 293T whole cell lysate/extract A. 30 µg 293T whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 µg of AMPK alpha-2 Polyclonal Antibody (Product # PA5-21494) 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha-2 Polyclonal Antibody (Product # PA5-21494) diluted at 1:1,000.
