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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF602 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat Jak1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse and rat Jak1 in Western blots.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P52332- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Ultraviolet radiation inhibits interleukin-2-induced tyrosine phosphorylation and the activation of STAT5 in T lymphocytes.
Kulms D, Schwarz T
The Journal of biological chemistry 2001 Apr 20;276(16):12849-55
The Journal of biological chemistry 2001 Apr 20;276(16):12849-55
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse/Rat Jak1 by Western Blot. Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line, Y3-Ag rat myeloid cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Jak1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF602) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Jak1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Jak1 in Mouse Splenocytes. Jak1 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse/Rat Jak1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF602) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
