K-J36

antibody from Rockland Immunochemicals, Inc.

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

K-J36

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#K-J36, RRID:AB_2614919

Product name

AKT3 Antibody Combo Pack - K-J36

Antibody type

Monoclonal

Vial size

1 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Mouse anti-AKT3 antibody (p/n 200-301-J36S). Lane 1: His-Tagged Recombinant AKT1 (p/n 009-001-I51). Lane 2: His-Tagged Recombinant AKT2 (p/n 009-001-E71). Lane 3: His-Tagged Recombinant AKT3 (p/n 009-001-E75). Load: 50 ng per lane. Primary antibody: Mouse anti-AKT3 antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase Conjugated Rabbit anti-Mouse secondary antibody (p/n 610-403-C46) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa for AKT-3.

Validation comment

Western Blot