38-3600

antibody from Invitrogen Antibodies

Targeting: APH1A APH-1A, CGI-78

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

38-3600

Provider

Invitrogen Antibodies

Product name

Anti-APH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.25 mg/ml

Storage

-20°C

References

Submitted references

EphB signaling regulates target innervation in the developing and deafferented auditory brainstem.

Nakamura PA, Hsieh CY, Cramer KS
Developmental neurobiology 2012 Sep;72(9):1243-55

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Gender- and age-dependent gamma-secretase activity in mouse brain and its implication in sporadic Alzheimer disease.

Placanica L, Zhu L, Li YM
PloS one 2009;4(4):e5088

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Conditional forebrain inactivation of nicastrin causes progressive memory impairment and age-related neurodegeneration.

Tabuchi K, Chen G, Südhof TC, Shen J
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Jun 3;29(22):7290-301

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Pen2 and presenilin-1 modulate the dynamic equilibrium of presenilin-1 and presenilin-2 gamma-secretase complexes.

Placanica L, Tarassishin L, Yang G, Peethumnongsin E, Kim SH, Zheng H, Sisodia SS, Li YM
The Journal of biological chemistry 2009 Jan 30;284(5):2967-77

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Transcriptional regulation of PEN-2, a key component of the gamma-secretase complex, by CREB.

Wang R, Zhang YW, Sun P, Liu R, Zhang X, Zhang X, Xia K, Xia J, Xu H, Zhang Z
Molecular and cellular biology 2006 Feb;26(4):1347-54

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Presenilin-1 uses phospholipase D1 as a negative regulator of beta-amyloid formation.

Cai D, Netzer WJ, Zhong M, Lin Y, Du G, Frohman M, Foster DA, Sisodia SS, Xu H, Gorelick FS, Greengard P
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 7;103(6):1941-6

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Nicastrin is critical for stability and trafficking but not association of other presenilin/gamma-secretase components.

Zhang YW, Luo WJ, Wang H, Lin P, Vetrivel KS, Liao F, Li F, Wong PC, Farquhar MG, Thinakaran G, Xu H
The Journal of biological chemistry 2005 Apr 29;280(17):17020-6

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of APH1A was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with APH1A Rabbit Polyclonal Antibody (Product # 38-3600) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.