15704-1-AP

antibody from Proteintech Group

Targeting: EIF4G1 EIF4F, EIF4G, p220, PARK18

Provider product page for 15704-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [40]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 15704-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15704-1-AP, RRID:AB_2261979
Product name: EIF4G1 antibody
Antibody type: Polyclonal
Description: EIF4G1 antibody (Cat. #15704-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

EIF4G1 protein structure - 15704-1-AP shown in red.

Submitted references Novel LncRNA Gm44763 Regulates Morphine-Induced Reward Memory via MiR-298-5p-Mediated eIF4E Translation Control.

Gao F, Yang X, Yang Z, Yu D, Zhang B, Wang Y, Yao Z, Chen J, Liao Q, Li L, Hou B, Wang D, Zhang Y, Yan C
Research (Washington, D.C.) 2026;9:1032

Coxsackievirus B3 Cleaves INTS10 Through 3C Protease to Facilitate Its Replication.

Yuan L, Lin L, Bi C, Niu X, Chen Y, Fei Y, Wang G, Wang H, Wang Y, Zhao W, Zhong Z, Lin L
International journal of molecular sciences 2026 Jan 19;27(2)

Translation factor eIF4G2 directs CD8(+) T cell lineage commitment by selectively enabling the IL-7 receptor response.

Cui J, Zhang X, Yang Y, Shan L, Li Y, Jiang L, Xie W, Jin T, Lang X
iScience 2026 Apr 17;29(4):115313

Processing bodies promote lysosomal quality control and cell survival during recovery from lysosomal damage.

Jia J, Duran J, Chen L, Pu J, Ivanov P
Research square 2025 Sep 9;

Ubiquilin-2 liquid droplets catalyze α-synuclein fibril formation.

Takei T, Sasazawa Y, Noshiro D, Kitagawa M, Kataura T, Hirawake-Mogi H, Kawauchi E, Nakano Y, Tashiro E, Saitoh T, Nishiyama S, Ogawa S, Kakuta S, Kazuno S, Miura Y, Taniguchi D, Korolchuk VI, Noda NN, Saiki S, Imoto M, Hattori N
The EMBO journal 2025 Nov;44(22):6527-6555

Targeting LARP1 to mitigate aging in lens epithelial cells: mechanistic insights into mitochondrial dysfunction.

Qi H, Sun L, Chai J, Ma J, Yu H, Xia M, Hu X
Experimental eye research 2025 Nov;260:110582

tiRNA: An efficient and controllable gene silencing technology via translation inhibition.

Xia B, Cai J, He Z, Zhu Q
New biotechnology 2025 Nov 25;89:177-190

E0703 targets ERβ to facilitate the upregulation of GLI3, thereby alleviating irradiation-induced DNA damage on lymphocytes.

Liao Z, Zhang L, Wu Z, Hu C, Tang X, Xiao C, Qian L, Gao Y
Cellular & molecular biology letters 2025 Nov 13;30(1):136

YTHDF1 differentiates the contributing roles of mTORC1 in aging.

Xu C, Yu C, Zhang J, Zhang Z, Zhang J, Meng Y, Wang M, Wen M, Chen J, Ning Y, Chen X, Gu J, Su W, Liu B
Molecular cell 2025 Jun 5;85(11):2194-2210.e8

The RNA-stability-independent role of the RNA m(6)A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance.

Liu T, Yang D, Wei Q, Wang Y, Tian L, Liu X, Yang Y, Luo Q, Xu J, Liu Y, Yang C, Zuo X, Luo F, Luo X, Zhao H, Li L, Xu J, Yu J, Ma S, Yu J, Yi P
Molecular cell 2025 Jun 19;85(12):2320-2336.e9

A circular RNA overcomes acquired resistance to BET inhibitors by antagonizing IGF2BP2-mediated c-MYC translation in TNBC.

Guo J, Li K, Ming Y, Pan Y, Tan S, Ma H, Chen S, Duan Y, Peng Y
Proceedings of the National Academy of Sciences of the United States of America 2025 Jul 8;122(27):e2504320122

N(6)-methyladenosine in 28S rRNA promotes oncogenic mRNA translation and tyrosine catabolism.

Chen B, Li L, Huang Y, Ma J, Ji F, Chen Y, Wu L, Peng H
Cell reports 2025 Jan 28;44(1):115139

The protein circPETH-147aa regulates metabolic reprogramming in hepatocellular carcinoma cells to remodel immunosuppressive microenvironment.

Lan T, Gao F, Cai Y, Lv Y, Zhu J, Liu H, Xie S, Wan H, He H, Xie K, Liu C, Wu H
Nature communications 2025 Jan 2;16(1):333

Ataxin-2-like promotes translation of nonpolyadenylated reovirus mRNA.

Somoulay X, Treadaway GS, Hedayati S, Steed ME, Adam NE, Williams MS, Parker JSL, Woolford JL, Silva LA, Taylor GM, Dermody TS
Nature communications 2025 Dec 17;17(1):842

Inhibition of K63 ubiquitination by G-Protein pathway suppressor 2 (GPS2) regulates mitochondria-associated translation.

Gao Y, Kwan J, Orofino J, Burrone G, Mitra S, Fan TY, English J, Hekman R, Emili A, Lyons SM, Cardamone MD, Perissi V
Pharmacological research 2024 Sep;207:107336

VP3 protein of Senecavirus A promotes viral IRES-driven translation and attenuates innate immunity by specifically relocalizing hnRNPA2B1.

Li L, Li X, Zhong H, Li M, Wan B, He W, Zhang Y, Du Y, Chen D, Zhang W, Ji P, Jiang D, Han S
Journal of virology 2024 Sep 17;98(9):e0122724

G3BP1 and SLU7 Jointly Promote Immune Evasion by Downregulating MHC-I via PI3K/Akt Activation in Bladder Cancer.

Zheng X, Chen J, Deng M, Ning K, Peng Y, Liu Z, Li X, Zhou Z, Tang H, Li Y, Kang T, Liu Z
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Feb;11(7):e2305922

Calcium signaling from damaged lysosomes induces cytoprotective stress granules.

Duran J, Salinas JE, Wheaton RP, Poolsup S, Allers L, Rosas-Lemus M, Chen L, Cheng Q, Pu J, Salemi M, Phinney B, Ivanov P, Lystad AH, Bhaskar K, Rajaiya J, Perkins DJ, Jia J
The EMBO journal 2024 Dec;43(24):6410-6443

Baicalein suppresses Coxsackievirus B3 replication by inhibiting caspase-1 and viral protease 2A.

Dong Y, Shao E, Li S, Wang R, Wang D, Wang L, Yang H, He Y, Luan T, Chen Y, Wang Y, Lin L, Wang Y, Zhong Z, Zhao W
Virologica Sinica 2024 Aug;39(4):685-693

Decreased RNA-binding protein heterogeneous nuclear ribonucleoprotein U improves multiple myeloma sensitivity to lenalidomide.

Lin Z, Zhang Y, Liu X, Luo H, Li Q, Gao Q, Wang X, Wen J, Li L, Feng Y, Wang F, Huang J, Zhai X, Zhang L, Niu T, Zheng Y
British journal of haematology 2024 Aug;205(2):594-606

A mechanism that transduces lysosomal damage signals to stress granule formation for cell survival.

Duran J, Poolsup S, Allers L, Lemus MR, Cheng Q, Pu J, Salemi M, Phinney B, Jia J
bioRxiv : the preprint server for biology 2024 Apr 2;

LINC02870 facilitates SNAIL translation to promote hepatocellular carcinoma progression.

Guo M, Zhuang H, Huang J, Shao X, Bai N, Li M, Niu M, Wei W, Sun L, Li Y, Qiang Z
Molecular and cellular biochemistry 2023 Sep;478(9):1899-1914

Stress granules plug and stabilize damaged endolysosomal membranes.

Bussi C, Mangiarotti A, Vanhille-Campos C, Aylan B, Pellegrino E, Athanasiadi N, Fearns A, Rodgers A, Franzmann TM, Šarić A, Dimova R, Gutierrez MG
Nature 2023 Nov;623(7989):1062-1069

5'-tRF-GlyGCC promotes breast cancer metastasis by increasing fat mass and obesity-associated protein demethylase activity.

Chen F, Song C, Meng F, Zhu Y, Chen X, Fang X, Ma D, Wang Y, Zhang C
International journal of biological macromolecules 2023 Jan 31;226:397-409

CDKAL1 Drives the Maintenance of Cancer Stem-Like Cells by Assembling the eIF4F Translation Initiation Complex.

Huang R, Yamamoto T, Nakata E, Ozaki T, Kurozumi K, Wei F, Tomizawa K, Fujimura A
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Apr;10(12):e2206542

Pancreatic β-cell hyper-O-GlcNAcylation leads to impaired glucose homeostasis in vivo.

Jo S, Pritchard S, Wong A, Avula N, Essawy A, Hanover J, Alejandro EU
Frontiers in endocrinology 2022;13:1040014

EIF4G1 Is a Potential Prognostic Biomarker of Breast Cancer.

Li K, Tan G, Zhang X, Lu W, Ren J, Si Y, Adu-Gyamfi EA, Li F, Wang Y, Xie B, Wang M
Biomolecules 2022 Nov 26;12(12)

Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation.

You B, Pan S, Gu M, Zhang K, Xia T, Zhang S, Chen W, Xie H, Fan Y, Yao H, Cheng T, Zhang P, Liu D, You Y
Journal of extracellular vesicles 2022 May;11(5):e12221

Construction and validation of a prognostic risk model for breast cancer based on protein expression.

Huang B, Zhang X, Cao Q, Chen J, Lin C, Xiang T, Zeng P
BMC medical genomics 2022 Jul 4;15(1):148

ADAR1 limits stress granule formation through both translation-dependent and translation-independent mechanisms.

Corbet GA, Burke JM, Parker R
Journal of cell science 2021 Sep 1;134(17)

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

Sun L, Wang W, Han C, Huang W, Sun Y, Fang K, Zeng Z, Yang Q, Pan Q, Chen T, Luo X, Chen Y
Molecular cell 2021 Nov 4;81(21):4493-4508.e9

RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site.

George J, Li Y, Kadamberi IP, Parashar D, Tsaih SW, Gupta P, Geethadevi A, Chen C, Ghosh C, Sun Y, Mittal S, Ramchandran R, Rui H, Lopez-Berestein G, Rodriguez-Aguayo C, Leone G, Rader JS, Sood AK, Dey M, Pradeep S, Chaluvally-Raghavan P
Cell reports 2021 Nov 2;37(5):109934

Inhibition of Antiviral Innate Immunity by Foot-and-Mouth Disease Virus L(pro) through Interaction with the N-Terminal Domain of Swine RNase L.

Sui C, Jiang D, Wu X, Liu S, Li F, Pan L, Cong X, Li J, Yoo D, Rock DL, Miller LC, Lee C, Du Y, Qi J
Journal of virology 2021 Jul 12;95(15):e0036121

Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.

Jo S, Lockridge A, Mohan R, Esch N, Schlichting R, Panigrahy N, Essawy A, Gustafson E, Alejandro EU
Diabetes 2021 Jan;70(1):155-170

ALS-Linked Mutant SOD1 Associates with TIA-1 and Alters Stress Granule Dynamics.

Lee DY, Jeon GS, Sung JJ
Neurochemical research 2020 Dec;45(12):2884-2893

EIF4G1 is a novel candidate gene associated with severe asthenozoospermia.

Sha Y, Liu W, Huang X, Li Y, Ji Z, Mei L, Lin S, Kong S, Lu J, Kong L, Zhu X, Lu Z, Ding L
Molecular genetics & genomic medicine 2019 Aug;7(8):e807

Cellular cap-binding protein, eIF4E, promotes picornavirus genome restructuring and translation.

Avanzino BC, Fuchs G, Fraser CS
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 5;114(36):9611-9616

Construction of a subgenomic CV-B3 replicon expressing emerald green fluorescent protein to assess viral replication of a cardiotropic enterovirus strain in cultured human cells.

Wehbe M, Huguenin A, Leveque N, Semler BL, Hamze M, Andreoletti L, Bouin A
Journal of virological methods 2016 Apr;230:1-8

SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation.

Hu S, Li J, Xu F, Mei S, Le Duff Y, Yin L, Pang X, Cen S, Jin Q, Liang C, Guo F
PLoS genetics 2015 Jul;11(7):e1005367

Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections.

Wu S, Wang Y, Lin L, Si X, Wang T, Zhong X, Tong L, Luan Y, Chen Y, Li X, Zhang F, Zhao W, Zhong Z
Virology journal 2014 Nov 20;11:192

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 15704-1-AP(EIF4G1 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using EIF4G1 antibody 15704-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 15704-1-AP(EIF4G1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 15704-1-AP(EIF4G1 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue